An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis – influence of auxins and cytokinins

Makunga, Nokwanda P.; Jäger, Anna K.; Staden, J. Van

doi:10.1007/s11240-005-1372-x

Cited by 21 publications

(12 citation statements)

References 19 publications

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“…The present study showed that the appropriate concentrations of combination of auxin, gibberellins and cytokinin are essential for the direct and efficient regeneration of explants without callus formation. The presence of a cytokinin in the medium is essential for adventitious shoot formation (Makunga et al 2005). The rate of regeneration was low on MS solid medium supplemented with single PGR whereas the regeneration rate was high on the MS solid medium supplemented with NAA in combination with BAP, zeatin and GA 3 (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

Dhital

Lim

Manandhar

2012

Nepal Journal of Science and Technology

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Response of widely grown potato cv. Superior and newly developed cvs. Gui valley and Bora valley to plant growth regulators (PGRs) for direct plant regeneration from internode, leaf blade and petiole explants were investigated. The explants were cultured on a MS solid medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), zeatin, indole-3-acetic acid (IAA) and gibberellic acid (GA 3 ). Potato cv. Superior, regenerated direct shoot without callus and root formation on MS solid medium supplemented with BAP or zeatin, proliferous roots were produced on NAA or IAA supplemented medium and only some calli were produced on GA 3 supplemented medium. The regeneration response varied with different concentrations of PGRs, singly and also in combinations. In the case of combined application of PGRs, the highest shoot regeneration (75.3%) and number of shoot per explant (11.5) and number of roots per explant (7.0) were obtained from the MS solid medium supplemented with zeatin (2 mg l -1 ), NAA (0.1 mg l -1 ) and GA 3 0.1 mg l -1 ). Among the three types of explants evaluated, internodes produced the highest number of shoots and roots for both potato cvs. Gui valley and Bora valley, and petiole produced the least number of shoots and roots. The regenerated shoots were rooted in PGRs-free MS solid medium and successfully established under glasshouse condition. Leaf, flower, and tuber morphology were identical to in vitro control and mother plants in the same conditions. This optimized regeneration system can be used for rapid shoot proliferation and also for gene transformation.

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Section: Resultsmentioning

confidence: 99%

Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

Dhital

Lim

Manandhar

2012

Nepal Journal of Science and Technology

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“…The latter PGR combination has been shown to induce hyperhydric plants (Makunga et al 2005) (see also Fig. 2B).…”

Section: Pre-acclimatizationmentioning

confidence: 89%

“…The first successful report on the tissue culture of T. garganica produced somatic embryos but plantlet production and acclimatization was not investigated (Jäger et al 1993). The only other reports on the successful regeneration of plantlets via tissue culture (Makunga et al 2003(Makunga et al , 2005) emanated from our laboratories and obstacles pertaining to low incidence of rooting of plantlets (32% rooted plantlets); reproducibility of the rooting regime; and, poor establishment of in vitro propagated plants after acclimatization as a result of fungal infections were reported on (Makunga et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Improved in vitro rooting and hyperhydricity in regenerating tissues of Thapsia garganica L.

Makunga

Jäger

Staden

2006

Plant Cell Tiss Organ Cult

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Stock cultures of Thapsia garganica grown on Murashige and Skoog agar medium (1962) (MS) (0.8% agar [w/v]; pH 5.8) with 0.5 mg l )1 NAA and 1.5 mg l )1 BA were best rooted by subjecting to half strength MS liquid medium with IBA (10 mg l )1 ) for 3 days prior to transfer to medium without plant growth regulators. A rooting frequency of 60% was noted with seven roots per rooted plant. Rooting was apparent after 10 days. The present study also aimed at reducing the occurrence of hyperhydric plants. The inclusion of 2% polyethylene glycol (w/v) in the growth medium or ventilation of cultures prior to acclimatization resulted in the production of plants true to type, closely resembling wild plants. Those plantlets that had been rooted and exposed to a better vented environment were able to acclimatize readily. Tissue culture propagation is therefore beneficial to the conservation of the medicinally important species, Thapsia garganica.

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“…halophilus are those of Imani et al (2001) and Thi and Pleschka (2005). As it is well-known, somatic embryogenesis was first achieved in carrot (Reinert 1958;Steward et al 1958), and since then, many reports about micropropagation and somatic embryogenesis have been published for carrot and other Apiaceae species (Ekiert 2000;Thorpe and Stasolla 2001;Makunga et al 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of the wild carrot Daucus carota L. subsp. halophilus (Brot.) A. Pujadas for conservation purposes

Tavares

Salgueiro

Canhoto

2009

In Vitro Cell.Dev.Biol.-Plant

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Daucus carota subsp. halophilus, is a wild crop relative of domestic carrot. It is an aromatic plant widely used in folk medicine due to recognized therapeutic properties of its essential oils. Experiments were carried out to evaluate the potential of in vitro propagation techniques to the conservation of this endemic and endangered taxon. The results showed that shoot tips of in vitro germinated seeds were able to proliferate in the presence of benzyladenine, with the best results being achieved using 4.4µM, both in the first and second cultures. Shoots rooted after being transferred to 1/2-Murashige and Skoog basal medium. The results indicated that the concentration of benzyladenine used during the multiplication phase did not interfere with the rate of root formation. The obtained plantlets were morphologically and anatomically identical to those obtained by seeds. Some of the in vitro produced shoots developed flowers that produced viable pollen. Plant regeneration was also achieved by somatic embryogenesis induction in cotyledons and root segments cultured in the presence of 4.5µM 2,4-dichlorophenoxyacetic acid. Somatic embryos converted into plantlets in a medium without growth regulators. Plants obtained either by shoot proliferation or somatic embryogenesis were acclimatized and are now growing at the Coimbra Botanical Garden. The first attempts to reintroduce these plants in the original habitat were successful. It can be concluded that the protocols developed are a useful approach to the conservation of this endemic species.

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An improved system for the in vitro regeneration of Thapsia garganica via direct organogenesis – influence of auxins and cytokinins

Cited by 21 publications

References 19 publications

Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

Improved in vitro rooting and hyperhydricity in regenerating tissues of Thapsia garganica L.

In vitro propagation of the wild carrot Daucus carota L. subsp. halophilus (Brot.) A. Pujadas for conservation purposes

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