In vitro culture and phenotypic and molecular characterization of gastric stem cells from human stomach

Abstract

This study opens new outlooks for investigation of gastric stem cell biology and pathobiology as well as host-H. pylori interactions.

A simple and cost‐efficient adherent culture platform for human gastric primary cells, as an in vitro model for Helicobacter pylori infection

“…To achieve this goal, long‐term cell culture systems have been established that used various enriched media (reviewed in Table ). Accordingly, a recent model was introduced by Garcia and colleagues, where they used enriched media to select for progenitor cells from gastric tissue specimens and expanded them in vitro. They presented their culture system as a model for short‐term study of the interaction of H. pylori with gastric progenitor cells.…”

“…These researchers used a homemade GI biomatrix to culture gastric tumor‐adjacent cells. Similarly, Garcia et al used MEF (3T3 cell line) to provide the above‐mentioned matrix/cell support for their cell culture system.…”

“…The use of gastrin for enrichment of gastric epithelial culture media, a common protocol for 3D cultures, dates back to 1973, when Miller et al used it to prolong their cell culture system for up to 6 months. On the other hand, Garcia et al have used various epidermal, hepatocyte, and fibroblast growth factors to select for and expand gastric progenitor cells. To generate a culture system for our 2D model, we used the 2D culture medium recommended by Schlaermann et al, which contained nicotinamide, EGF, ROCK inhibitor (Y‐27632), and TGF‐β inhibitor (A83‐01).…”

“…Another underinvestigated issue is the status of the donor host, in regard to H. pylori infection and gastric histopathology. To isolate and cultivate totally naive and healthy cells, we and others have collected biopsies from H. pylori ‐negative subjects, who presented normal gastric histopathology.…”

“…Accordingly, a recent model was introduced by Garcia and colleagues, 40 where they used enriched media to select for progenitor cells from gastric tissue specimens and expanded them in vitro.…”