Inflammatory signaling pathways induced by<i>Helicobacter pylori</i>in primary human gastric epithelial cells

Tran, Cong Tri; Garcia, Magali; Garnier, Martine; Burucoa, Christophe; Bodet, Charles

doi:10.1177/1753425916681077

Cited by 28 publications

(27 citation statements)

References 39 publications

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“…Previous reports have shown that berberine exerts a clearance effect and reduces gastric inflammation during epithelial cell signalling in H. pylori infection [ 23 ]. Based on this network, the targets associated with epithelial cell signalling are EGFR and CXCL8, which are important responses to H. pylori infection [ 24 – 26 ]. Helicobacter pylori infection may upregulate the expression of EGFR and CXCL8 through the cell response to epidermal growth factor stimulus (GO 0071364) and the response to bacterial molecules (GO 0002237), respectively.…”

Section: Resultsmentioning

confidence: 99%

Exploring the potential pharmacodynamic material basis and pharmacologic mechanism of the Fufang-Xialian-Capsule in chronic atrophic gastritis by network pharmacology approach based on the components absorbed into the blood

Li¹,

Xu²,

Liu³

et al. 2018

R. Soc. open sci.

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In this study, a new network pharmacology approach based on the components absorbed into the blood was used to investigate the pharmacodynamic material basis and the pharmacologic mechanism of the Fufang-Xialian-Capsule (FXL) in treating chronic atrophic gastritis (CAG). Initially, we confirmed the components absorbed into the blood by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Then, the network approach, which was based on the results of components absorbed into the blood, was used to analyse the pharmacodynamic material basis and the pharmacologic mechanism of FXL on treating CAG. As a result, 22 absorbed components were found in rat plasma. Given the results of the absorption analysis of the components, eight pathways associated with CAG development were found. The targets linked to these pathways are the drug targets of FXL in CAG treatment. The components associated with these targets are the potential pharmacodynamic material basis and exert synergy in regulating pathways during CAG treatment.

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Section: Resultsmentioning

confidence: 99%

Exploring the potential pharmacodynamic material basis and pharmacologic mechanism of the Fufang-Xialian-Capsule in chronic atrophic gastritis by network pharmacology approach based on the components absorbed into the blood

Li¹,

Xu²,

Liu³

et al. 2018

R. Soc. open sci.

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“…All these markers decreased significantly when cells were treated with AAF/II in the presence of TLR4 antagonist (Figure 4). GRO is a chemokine involved in the recruitment of neutrophils in several bacterial and non-bacterial infections including H. pylori (Tran et al, 2017), E. coli (Lee et al, 2018), B. cereus (Coburn et al, 2018), rhinovirus (Jamieson et al, 2019) and Leishmania (Ronet et al, 2018). Moreover, it has been shown to be associated with neutrophil recruitment mediated by the Th17 response (Fujie et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

TLR4 Participates in the Inflammatory Response Induced by the AAF/II Fimbriae From Enteroaggregative Escherichia coli on Intestinal Epithelial Cells

Alvestegui

Olivares-Morales

Muñoz

et al. 2019

Front. Cell. Infect. Microbiol.

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Enteroaggregative Escherichia coli (EAEC) infections are one of the most frequent causes of persistent diarrhea in children, immunocompromised patients and travelers worldwide. The most prominent colonization factors of EAEC are aggregative adherence fimbriae (AAF). EAEC prototypical strain 042 harbors the AAF/II fimbriae variant, which mediates adhesion to intestinal epithelial cells and participates in the induction of an inflammatory response against this pathogen. However, the mechanism and the cell receptors implicated in eliciting this response have not been fully characterized. Since previous reports have shown that TLR4 recognize fimbriae from different pathogens, we evaluated the role of this receptor in the response elicited against EAEC by intestinal cells. Using a mutual antagonist against TLR2 and TLR4 (OxPAPC), we observed that blocking of these receptors significantly reduces the secretion of the inflammatory marker IL-8 in response to EAEC and AAF/II fimbrial extract in HT-29 cells. Using a TLR4-specific antagonist (TAK-242), we observed that the secretion of this cytokine was significantly reduced in HT-29 cells infected with EAEC or incubated with AAF/II fimbrial extract. We evaluated the participation of AAF/II fimbriae in the TLR4-mediated secretion of 38 cytokines, chemokines, and growth factors involved in inflammation. A reduction in the secretion of IL-8, GRO, and IL-4 was observed. Our results suggest that TLR4 participates in the secretion of several inflammation biomarkers in response to AAF/II fimbriae.

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“…Cortex magnolia officinalis is another herb that has been proven to have anti-HP activity [ 18 , 20 ]. The activation of the inflammatory signaling pathways plays a crucial role in the pathogenicity of HP [ 21 ]. The inhibition of HP and the suppression of the HP-related inflammatory signaling pathways might be important underlying mechanisms for QHY efficacy.…”

Section: Discussionmentioning

confidence: 99%

Confirming the Effects of Qinghuayin against Chronic Atrophic Gastritis and a Preliminary Observation of the Involved Inflammatory Signaling Pathways: An In Vivo Study

Huang

Chen

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Background Qinghuayin (QHY) is a Chinese formula that is widely used in the treatment of chronic atrophic gastritis (CAG). This study was planned with the following objectives: (1) confirming the efficacy of QHY in a rat model of CAG and (2) performing a preliminary observation of the changes in several inflammatory signaling pathways potentially involved in the QHY mechanisms. MethodsA total of 33 rats were used in this study; they were divided into the control (n = 12) and model (n = 21) groups. QHY was administrated to both the groups. We assessed the pathological manifestations and the serum tumor necrosis factor alpha (TNF-α) level as markers of efficacy. We also performed a preliminary observation of the changes in the protein and messenger ribonucleic acid (mRNA) expression of toll-like receptors 4 (TLR4), MyD88, NF-κB, and COX-2. Results The pathological changes induced in the rats by the establishment of the CAG models were recovered by low and high doses of QHY. Their serum TNF-α level also reduced following low- and high-dose QHY treatment. Protein and mRNA expressions of TLR4, MyD88, NF-κB, and COX-2 were upregulated by the establishment of CAG models and downregulated by the administration of low- and high-dose QHY. Conclusions Our data confirm the efficacy of QHY as an adjuvant therapy, based on the theories in traditional Chinese medicine. The preliminary observations indicate that the downregulation of the enhanced inflammatory signaling pathways might be crucial QHY mechanisms that need further verification.

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Inflammatory signaling pathways induced byHelicobacter pyloriin primary human gastric epithelial cells

Cited by 28 publications

References 39 publications

Exploring the potential pharmacodynamic material basis and pharmacologic mechanism of the Fufang-Xialian-Capsule in chronic atrophic gastritis by network pharmacology approach based on the components absorbed into the blood

Exploring the potential pharmacodynamic material basis and pharmacologic mechanism of the Fufang-Xialian-Capsule in chronic atrophic gastritis by network pharmacology approach based on the components absorbed into the blood

TLR4 Participates in the Inflammatory Response Induced by the AAF/II Fimbriae From Enteroaggregative Escherichia coli on Intestinal Epithelial Cells

Confirming the Effects of Qinghuayin against Chronic Atrophic Gastritis and a Preliminary Observation of the Involved Inflammatory Signaling Pathways: An In Vivo Study

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