In vitro culture: an epigenetic challenge for plants

Us-Camas, Rosa; Rivera-Solís, Gustavo; Duarte-Aké, Fátima; De‐la‐Peña, Clelia

doi:10.1007/s11240-014-0482-8

Cited by 109 publications

(63 citation statements)

References 154 publications

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“…This can be a source of stress for the plants ( Us-Camas et al 2014). Most in vitro cultures are characterized by low light intensity, increased humidity, poor gas exchange and the presence of sugars in the culture medium.…”

Section: Discussionmentioning

confidence: 99%

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

et al. 2017

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Rumex tianschanicus 9 Rumex patientia is a high-biomass-yielding plant suitable for fuel and biogas production. The protocol of the hybrid sorrel micropropagation was used to study the changes in the photosystem II (PSII) activity as well as to analyse the ultrastructure of the chloroplasts. The lowest effective PSII quantum yield [Y(II)] and an apparent electron transport rate of PSII [ETR(II)] were observed for adventitious shoots that had been regenerated in vitro, before rooting. These fluorescence parameters were higher and similar for both the leaves of the same adventitious shoots that had been rooted under in vitro conditions and for the shoots that had been acclimated and grown in ex vitro conditions. The analysis indicated that the PSII activity strongly depends on the formation of properly functioning roots and that in vitro or ex vitro culture conditions are, at least to some degree, less important. TEM analysis revealed that chloroplasts from plants rooted in vitro were sufficiently mature and acclimatization processes have less impact on their development. This is the first report concerning the analysis of PSII activity and the ultrastructure of the chloroplasts at all of the stages of micropropagation, i.e. adventitious shoot formation in vitro, rooting in vitro and acclimation to ex vitro conditions. It strongly indicated that rooting under in vitro conditions, rather than the acclimation to ex vitro conditions, plays a key role in the development of a completely functional photosynthetic apparatus in hybrid sorrel.Keywords Rumex tianschanicus 9 Rumex patientia Á PSII activity Á Chlorophyll a fluorescence Á Ultrastructure of chloroplasts Á In vitro/ex vitro transfer Á Photosynthetic apparatus

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Section: Discussionmentioning

confidence: 99%

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

et al. 2017

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“…Epigenetics has provided a new layer of complexity to the understanding of gene regulation for agricultural applications (Boyko and Kovalchuk, 2013;Us-Camas et al, 2014). Epigenetics are typically studied by three different mechanisms: DNA methylation, histone modification, and small (sRNA; 50-250 nucleotides) or micro (19-25 nucleotides) noncoding RNAs.…”

Section: Epigenomicsmentioning

confidence: 99%

Biotic Interactions in the Rhizosphere: A Diverse Cooperative Enterprise for Plant Productivity

De‐la‐Peña

Loyola‐Vargas

2014

PLANT PHYSIOLOGY

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Microbes and plants have evolved biochemical mechanisms to communicate with each other. The molecules responsible for such communication are secreted during beneficial or harmful interactions. Hundreds of these molecules secreted into the rhizosphere have been identified, and their functions are being studied in order to understand the mechanisms of interaction and communication among the different members of the rhizosphere community. The importance of root and microbe secretion to the underground habitat in improving crop productivity is increasingly recognized, with the discovery and characterization of new secreting compounds found in the rhizosphere. Different omic approaches, such as genomics, transcriptomics, proteomics, and metabolomics, have expanded our understanding of the first signals between microbes and plants. In this review, we highlight the more recent discoveries related to molecules secreted into the rhizosphere and how they affect plant productivity, either negatively or positively. In addition, we include a survey of novel approaches to studying the rhizosphere and emerging opportunities to direct future studies.

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“…The morphogenetic potential of Capsicum sp. has been reported earlier, but the process of regeneration is slow and generally produces low frequencies of shoot regeneration, thus it has been found to be highly recalcitrant (Heidmann et al, 2011;Kothari et al, 2010;Us-Camas et al, 2014). Development of a specific body plan during embryogenesis requires the coordination of cell fates according to their positions along the embryo axes (Breuninger et al, 2008).…”

Section: Introductionmentioning

confidence: 98%

“…The absence of a functional shoot and the embryo incapacity to convert into plants is how the recalcitrance in Capsisum sp. manifests (Aboshama, 2011;Heidmann et al, 2011;Kothari et al, 2010;Us-Camas et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.)

Valle-Gough¹,

Avilés-Viñas²,

López-Erosa³

et al. 2015

Afr. J. Biotechnol.

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In order to determine the role of polyamines in the formation and development of the somatic embryos of Capsicum chinense, the effect of different concentrations (0, 0.01, 0.1, and 1.0 mM) of Putrescine, Spermidine and Spermine on the efficiency and morphology of the embryos was evaluated. The results show that none of the three polyamines evaluated had a significant effect on the number of embryos formed, except Spermidine (1 mM), which caused a significant reduction in their numbers, in comparison with the control treatment. However, the most noteworthy result was observed in the treatment containing 0.1 mM of Spermine. The embryos developed in this treatment showed harmonic apex-radicle development, pale-green coloration and the formation of two tiny cotyledonary leaves. Real-time PCR was used to analyze the differential expression of the WUS, WOX1 and WOX3 genes in somatic embryos treated with Spermine and untreated, including the zygotic embryo. The transcript levels of the genes analyzed were found to differ significantly between both types of embryos (somatic and zygotic), with the zygotic embryos presenting a higher level of transcripts; however, compared to the untreated somatic embryos, the somatic embryos treated with Spermine showed an increase in the transcript levels of the three genes analyzed (WUS, WOX1 and WOX3); the WOX1 gene in particular presented an accumulation pattern similar to that of the zygotic embryo of the species.

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In vitro culture: an epigenetic challenge for plants

Cited by 109 publications

References 154 publications

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

Biotic Interactions in the Rhizosphere: A Diverse Cooperative Enterprise for Plant Productivity

Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.)

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