In vitro cultivation of tansy (Tanacetum vulgare L.): a tool for the production of potent pharmaceutical agents

Abstract

In this study, tansy (Tanacetum vulgare L.) in vitro culture was established from seeds collected from natural populations. The multiplication of plantlets was conducted through shoot tips that exhibited potent apical growth and regeneration capacities on basal medium (BM), without the addition of any plant growth regulators (PGRs). PGRs were also omitted for the establishment and cultivation of tansy root cultures. Both abaxial and adaxial leaf surfaces of in vitro micropropagated plantlets were covered with glandular biseriate trichomes. Histochemical staining showed that glandular secretions were rich in lipid and terpene compounds, confirmed by GC-MS analysis of essential oil (EO). In the total EO, similar portions of oxygenated monoterpenes (38.5% m/m) and oxygenated sesquiterpenes (22.6% m/m) were detected. Chemical profiles of methanol extracts of in vitro cultured tansy shoots and roots varied in quantity and quality from those obtained from wild-growingtansy. HPLC analysis indicated that the methanol extracts of in vitro cultured roots were the richest in 3,5-O-dicaffeoylquinic acid (3,5-O-DCQA), in which the concentration was 6 times higher (10.220 mg/g DW) than that in the extract obtained from roots of wild-growing tansy (1.684 mg/g DW). This result is noticeable in the manner of industrial production of biologically active 3,5-O-DCQA that has been shown to have antioxidant, hepatoprotective, antiviral, antimutagenic, and immunomodulatory activity. Biotechnological interventions on secondary metabolite production taking place in trichomes could further enhance the production of some important tansy metabolites and further investigation will be directed toward the elucidation of the pharmaceutical potential of tansy in vitro obtained metabolites, as mixtures or single moieties.

In Vitro Study of Biological Activity of Tanacetum vulgare Extracts

“…The research by Devrnja et al [ 1 ] enabled it to be possible to establish that the extract of T. vulgare has the ability to inhibit biofilm synthesis and also exhibits antioxidant and antimicrobial properties. The same research group showed the possibility of using T. vulgare extract in the pharmaceutical and cosmetic industries [ 1 ].…”

“…The name Tanacetum vulgare L., also known as Common Tansy, is derived from the Greek word “athanasia”, which means “immortality”, most likely as a result of the fact that the flowers of this plant do not wilt when dried [ 1 ]. This perennial herbaceous plant is widely distributed in North America, Europe, Asia, China, Japan, North Korea, and Russia.…”

Chemical Profiling and Antioxidant Activity of Tanacetum vulgare L. Wild-Growing in Latvia

“…Plant extracts and essential oil of T. vulgare are known for their biological activities, mainly antioxidant, antimicrobial, insecticidal, cytotoxic, antivirus, and anti-inflammatory activities [ 6 , 7 ]. The extracts of T. vulgare have been reported to have anticancer properties and an antiproliferative effect on human cervical adenocarcinoma (HeLa) cells in the micrograms range [ 6 , 8 ]. However, pharmaceutical activities are mainly attributable to specific compounds, such as sesquiterpene lactones, volatile oil, flavonoids, and phenolic acids [ 4 , 9 , 10 ].…”

Jasmonates promote enhanced production of bioactive caffeoylquinic acid derivative in Eclipta prostrata (L.) L. hairy roots

“…Eclipta prostrata (L.) L. produces phenolic acids such as chlorogenic acid and its derivative 3,5-di- O -caffeoylquinic acid (3,5-diCQA) (Lee et al 2010 ). Chlorogenic acid and its derivatives have demonstrated several biological activities, such as antioxidant, antibiosis, anti-inflammatory, antivirus, and antitumor activities (Huang et al 2014 ; Ali et al 2017 ; Liu et al 2018 ; Devrnja et al 2020 ). More recently, studies by molecular docking using a serine-type protease (Mpro), a SARS-CoV-2 virus polyprotein, showed that the obtained binding energies of 3,5-diCQA were closed to remdesivir (antiviral compound), indicating stronger binding to Mpro protein and promising antiviral compounds that could treat Covid-19 (Shah et al 2021 ).…”