Jasmonates promote enhanced production of bioactive caffeoylquinic acid derivative in Eclipta prostrata (L.) L. hairy roots

Maciel, Geveraldo; Lopes, Adriana A.; Cantrell, Charles L.; França, Suzelei de Castro; Bertoni, Bianca Waléria; Lourenço, Miriam Vergínia

doi:10.1007/s11240-021-02201-4

Cited by 15 publications

(9 citation statements)

References 38 publications

(42 reference statements)

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“…In a similar study on hairy root cultures of Eclipta prostrata , MeJA greatly improved the CQAs production [ 74 ]. A growth in CGA and its derivatives in response to MeJA and SA in Gardenia jasminoides cell suspensions was also observed [ 24 ], although no effect of MeJA or SA on the biosynthesis of diCQAs was detected in Cynara cardunculus intact leaves [ 75 ].…”

Section: Resultsmentioning

confidence: 99%

Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Vasyutkina

Yugay

Grigorchuk

et al. 2022

IJMS

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Ipomoea batatas is a vital root crop and a source of caffeoylquinic acid derivatives (CQAs) with potential health-promoting benefits. As a naturally transgenic plant, I. batatas contains cellular T-DNA (cT-DNA) sequence homologs of the Agrobacterium rhizogenes open reading frame (ORF)14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ORF17n of unknown function. This study aimed to evaluate the effect of abiotic stresses (temperature, ultraviolet, and light) and chemical elicitors (methyl jasmonate, salicylic acid, and sodium nitroprusside) on the biosynthesis of CQAs and cT-DNA gene expression in I. batatas cell culture as a model system. Among all the applied treatments, ultraviolet irradiation, methyl jasmonate, and salicylic acid caused the maximal accumulation of secondary compounds. We also discovered that I. batatas cT-DNA genes were not expressed in cell culture, and the studied conditions weakly affected their transcriptional levels. However, the Ib-rolB/C gene expressed under the strong 35S CaMV promoter increased the CQAs content by 1.5–1.9-fold. Overall, our results show that cT-DNA-encoded transgenes are not involved in stress- and chemical elicitor-induced CQAs accumulation in cell cultures of I. batatas. Nevertheless, overaccumulation of RolB/RolC transcripts potentiates the secondary metabolism of sweet potatoes through a currently unknown mechanism. Our study provides new insights into the molecular mechanisms linked with CQAs biosynthesis in cell culture of naturally transgenic food crops, i.e., sweet potato.

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Section: Resultsmentioning

confidence: 99%

Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Vasyutkina

Yugay

Grigorchuk

et al. 2022

IJMS

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“…Eclipta prostrata (L.) L. hairy roots C19 clone (Diogo et al 2009 ; Maciel et al 2021 ) have been cultivated in MS liquid culture medium under agitation (100 rpm) at 25 ± 1 ºC in the dark.…”

Section: Methodsmentioning

confidence: 99%

“…WL reduces pulmonary fibrosis (Yang et al 2019 ), attenuates doxorubicin-induced inflammation (Zhu et al 2019 ), is promising agent in the mantle cell lymphoma treatment (Romanchikova and Trapencieris 2019 ), exhibits strong antioxidant activity (Li et al 2020 ), may be a promising anti-inflammatory candidate to combat Pseudomonas aeruginosa keratitis (Xu et al 2021 ) and reduces acute pancreatitis and associated lung injury (Fan et al 2021 ). In previous work was investigated the suitability of E. prostrata (L.) L. hairy root cultures biosynthesize the 3,5-di- O -caffeoylquinic acid (3,5-diCQA) (Maciel et al 2021 ), a promising biological compound against SARS-CoV-2, the virus that causes covid-19 (Joshi et al 2020 ; Shah et al 2021 ; Sumon et al 2021 ; Kadioglu et al 2021 ). Although WL, DWL and 3,5-diCQA are known, their biosynthesis of compounds from E. prostrata (L.) L. are not related in the literature.…”

Section: Introductionmentioning

confidence: 99%

Biosynthetic studies through feeding experiments in Eclipta prostrata (L.) L. hairy roots

Lopes

Souza

França

et al. 2022

Plant Cell Tiss Organ Cult

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Eclipta prostrata (L.) L. is a medicinal plant of the Asteraceae family, and several extracts and isolated compounds of E. prostrata (L.) L. showed a wide range of biological activities such as antimicrobial, anticancer, hepatoprotective, neuroprotective, hair growth promoting activities, and more recently against covid-19. Eclipta prostrata (L.) L. hairy roots produce wedelolactone (WL), demethylwedelolactone (DWL) and 3,5-di- O -caffeoylquinic acid (3,5-diCQA), and there is no data in literature regarding biosynthetic pathways are involved. To verify the biosynthetic route, feeding experiments were carried out using sodium [2- 13 C]acetate, [3- 13 C] dl -phenylalanine, and 13 C-labeled compounds (WL, DWL and 3,5-diCQA) were detected by ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-QTOF-MS). Analysis showed that the metabolic pathways operative of coumestans (WL and DWL) are derived from acetate and shikimate pathways, while that the phenylpropanoid (3,5-diCQA) biosynthesis is exclusively from shikimate pathway. Supplementary Information The online version contains supplementary material available at 10.1007/s11240-022-02342-0.

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“…No variation was detected with the 6 SSR primers in the regenerated plantlets of A. chinensis at the DNA level. , it has also been reported to be used for explant sterilization (Maciel et al 2022). Considering these, we analyzed the effects of the three sterilants at different time, and the results illustrated that although the overall sterilization effect of 0.1% MC was superior to that of 2% SH and 0.05% CD, the regeneration rate of the explants after inoculation was insu cient, indicating that 0.1% MC produced greater damage the explants than that of the other two compounds, with 0.05% CD causing little harm to the explants.…”

Section: Ssr Analysis Of Genetic Stabilitymentioning

confidence: 99%

In vitro adventitious regeneration and plantlet transplantation of Atractylodes chinensis (DC.) Koidz., a valuable medicinal plant

Jia

Rong

Zhang

et al. 2023

Preprint

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Triploid Atractylodes chinensis (DC.) Koidz. (A. chinensis) is a valuable genetic resource for medicinal plant breeding, and there is a demand to develop an efficient in vitro regeneration protocol to resolve triploid sterility in production. In this study, the effects of sterilization, bud type, 6-benzyladenine (6-BA) combined with 1-naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA) and transplanting materials on shoot regeneration, rooting and growth were explored. The genetic stability of regenerated plants was verified using flow cytometry (FCM) and simple sequence repetition (SSR) molecular markers. When axillary buds were sterilized with sodium hypochlorite for 30 min and cultured on Murashige and Skoog (MS) + 1.5 mg·L-1 6-BA + 0.4 mg·L-1 NAA medium, the regeneration rate was 61.33%. Strong roots occurred on 1/2 MS combined with 3% sucrose and 0.6 mg·L-1 IBA medium. Rooting plantlets transplanted into a mixture of peat soil: perlite (1:1, v/v), the survival rate up to 90.00%. Histological analyses revealed that shoot meristematic cells derived from cambium cells developed into the apical meristem and formed shoots. FCM analysis determined that the ploidy levels of 20 random regenerated plants were coincident with explants, the number and size of bands obtained by 6 pairs of SSR polymorphic primers were consistent. The results of this study show that our in vitro adventitious regeneration and plantlet transplantation protocol of triploid A. chinensis was efficient and produced strong genetic stability.

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Jasmonates promote enhanced production of bioactive caffeoylquinic acid derivative in Eclipta prostrata (L.) L. hairy roots

Cited by 15 publications

References 38 publications

Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Biosynthetic studies through feeding experiments in Eclipta prostrata (L.) L. hairy roots

In vitro adventitious regeneration and plantlet transplantation of Atractylodes chinensis (DC.) Koidz., a valuable medicinal plant

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