1978
DOI: 10.1007/bf02617972
|View full text |Cite
|
Sign up to set email alerts
|

In vitro cultivation of human renal cell cancer

Abstract: Two cell lines derived from primary human renal-cell cancers (RCC) have been established and characterized. Cell line 786-O has been in culture for longer than 1 year and has been subcultured more than 50 times. It has a doubling time of 45 hr and a hypertriploid karyotype and possesses a Y chromosome. Cell line 769-P also has been in culture for longer than 1 year. It has been subcultured 50 times and has a doubling time of 35 hr and a hypodiploid karyotype. Cells from both lines are epithelial, and they prod… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

1
28
0

Year Published

1981
1981
2017
2017

Publication Types

Select...
6
2

Relationship

0
8

Authors

Journals

citations
Cited by 56 publications
(29 citation statements)
references
References 9 publications
1
28
0
Order By: Relevance
“…For the in vivo studies, the mouse osteosarcoma LM8 (23), human osteosarcoma SaOS2 (24), human fibrosarcoma HT1080 (25), human synovial sarcoma Syo-1 (26), human renal cancer 786-O (27), human prostate cancer PC-3 (28) and human lung cancer A549 (29) cell lines were used. LM8, SaOS2, Syo-1, 786-O, and A549 cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Nacalai Tesque, Inc., Kyoto, Japan) containing 10% fetal calf serum (FCS) (Nacalai Tesque, Inc.) and 100 U/ml penicillin G and 100 mg/ml streptomycin antibiotics (Nacalai Tesque, Inc.).…”
Section: Methodsmentioning
confidence: 99%
“…For the in vivo studies, the mouse osteosarcoma LM8 (23), human osteosarcoma SaOS2 (24), human fibrosarcoma HT1080 (25), human synovial sarcoma Syo-1 (26), human renal cancer 786-O (27), human prostate cancer PC-3 (28) and human lung cancer A549 (29) cell lines were used. LM8, SaOS2, Syo-1, 786-O, and A549 cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Nacalai Tesque, Inc., Kyoto, Japan) containing 10% fetal calf serum (FCS) (Nacalai Tesque, Inc.) and 100 U/ml penicillin G and 100 mg/ml streptomycin antibiotics (Nacalai Tesque, Inc.).…”
Section: Methodsmentioning
confidence: 99%
“…To generate standard curves for quantitative analysis of each gene, cell lines that were reliable and had verified sources of mRNA for the particular gene were chosen. Tissue RNA was extracted as described above, and total RNA for Axl and Gas6 standards was extracted from cultured RCC 786-O cells (32) and stable Gas6-transfected HEK293 cells (33), respectively, using CellDirect One-Step qRT-PCR kit (Invitrogen). A master mix of Taqman MGB primers and…”
Section: Methodsmentioning
confidence: 99%
“…The patient had a total serum calcium of 10.6-12.5 mg/dl, albumin 3.0 g/dl, and phosphorus 3.5 mg/dl. The culture methods and a complete characterization of the cell line have previously been published (3).…”
Section: Methodsmentioning
confidence: 99%