Human renal carcinoma cells produce hypercalcemia in the nude mouse and a novel protein recognized by parathyroid hormone receptors.

Parathyroid hormonelike protein from human renal carcinoma cells. Structural and functional homology with parathyroid hormone.

“…The human renal carcinoma cell line 786-0 was obtained from a hypercalcemic patient and produces hypercalcemia when grown as xenografts in the nude mouse (4). Here we report that a purified protein from 786-0 cultures binds to high affinity PTH receptors in renal plasma membranes and mimics the physiologic effects of PTH in renal cells and bone explants.…”

“…Recently, squamous and renal carcinomas have been shown to secrete proteins that, like PTH, activate adenylate cyclase in bone and kidney, yet are immunochemically distinct from PTH (4,5). These proteins may also reproduce some of the physiological effects of PTH, as partially purified preparations have bone-resorbing (6, 7) and phosphaturic activity (8).…”

A new human breast cancer cell line, KPL-3C, secretes parathyroid hormone-related protein and produces tumours associated with microcalcifications in nude mice

“…No such microcalcifications were observed in those tumours. (Ellison et al, 1975;Strewler et al, 1983;Sato et al, 1987;Merryman et al, 1989;Miyake et al, 1991;De Miguel and Esbrit, 1992;Ichinose et al, 1993;Tabuenca et al, 1995;Birch et al, 1995). Although immunohistochemical and molecular analyses clearly demonstrate that the majority of breast cancer cells express PTHrP in primary tumours and metastatic sites and, in particular, in bone metastasis (Southby et al, 1990;Powell et al, 1991;Bundred et al, 1992;Vargus et al, 1992;Bouizar et al, 1993;Kohno et al, 1994a,b), only a little experimental data have been published so far concerning PTHrP secretion from established breast cancer cell lines (Tabuenca et al, 1995;Birch et al, 1995 (Martin, 1988;Mundy, 1990).…”

The Tumor Cells (FA-6) Established from a Pancreatic Cancer Associated with Humoral Hypercalcemia of Malignancy: A Simultaneous Production of Parathyroid Hormone-Like Activity and Transforming Growth Factor Activity.

“…Although various animal (Sica et al, 1983;D'Souza et al, 1984;Rosol et al, 1986) and human (Strewler et al, 1983;Bringhurst et al, 1986;Nissenson et al, 1985;Sato et al, 1987a, b) tumor cells associated with HHM have been cultured, cells originating in exocrine pancreatic cancer have not been established in vitro. In the present study, tumor cells were selected from an undifferentiated pleomorphic adenocarcinoma of exocrine pancreas associated with HHM which was injected into nude mice and re- Albumin (66 kDa), carbonic anhydrase (29 kDa), cytochrome C (12.4 kDa), and aprotinin (6.5 kDa) were used as markers of mole;ular size.…”