In vitro cormlet production and growth evaluation under greenhouse conditions in saffron (<i>Crocus sativus</i> L.) – A commercially important crop

Devi, Kiran; Sharma, Madhu; Singh, Mandeep; Ahuja, Paramvir Singh

doi:10.1002/elsc.201000080

Cited by 31 publications

(10 citation statements)

References 14 publications

(14 reference statements)

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“…2e). Recently, Devi et al 21 reported the vegetative growth (55%) was noticed from the corms of mean weight 2.5 g while the corms of 2.0 g mean weight showed 40% vegetative growth and 19% flowering and the corms of mean weight 1.5 g exhibited only vegetative growth (25%) (table 5 and Figs. 2G-i).…”

Section: Resultsmentioning

confidence: 96%

In vitro cormlet production of saffron (Crocus sativus L. Kashmirianus) and their flowering response under greenhouse

et al. 2012

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A complete protocol for the saffron cormlet production under in vitro conditions and subsequent flowering under greenhouse conditions is described. Highest number of cormlets (70.0 ± 0.30) per corm slice (explant) could be regenerated on Murashige and Skoog (MS) half strength medium supplemented with thidiazuron (TDZ) (20 µM), Indole acetic acid (IAA) (10 µM), and sucrose (40 g/l). Maximum germination (90%) of these cormlets could be achieved on MS medium containing 6-benzyl amino purine (BAP) (20 µM) and α-naphthalene acetic acid (NAA) (15 µM). In order to increase the size of the in vitro raised cormlets, these were cultured on MS medium containing TDZ (15 µM) and IAA in the range of 1.5-30 µM. Maximum increase in cormlet size could be attained on TDZ (15 µM) + IAA (12.5 µM) + sucrose (30 g/l), and the average size of cormlets was 2.5g. In another experiment, apical vegetative buds of actively growing corms were cultured for cormlet development, and corms of size 2.5g could be developed on MS medium with NAA (15 µM), BAP (20 µM), and sucrose (30 g/l). The in vitro developed cormlets were dried under shade at 25 ± 2°C for 7 d. These were then planted in small cups containing clay loam soil and kept in green house at 20 ± 2°C. In vitro developed cormlets with mean weight 2.5 g showed maximum flowering (25%) as well as vegetative growth (55%), while only 19% cormlets of 2.0 g flowered. To our knowledge this is the first report on successful flowering from in vitro raised cormlets under greenhouse.

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Section: Resultsmentioning

confidence: 96%

In vitro cormlet production of saffron (Crocus sativus L. Kashmirianus) and their flowering response under greenhouse

et al. 2012

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“…Additionally at concentration of 10 mg L -1 paclobutrazol caused an increase in daughter corm number and healthy daughter corm yield statistically than control and the other treatments (Table 1, 2; Figure 1.g). The results are supported by Devi et al (2011). In their study 1.7 µM and 3.4 µM paclobutrazol gave higher saffron corm production respectively than the other treatments in in vitro.…”

Section: Resultsmentioning

confidence: 64%

“…On the other hand there are a few studies (Aytekin and Acıkgoz, 2008) related with usage of plant growth regulators for saffron corm production under the field condition. Mostly the studies (Plessner et al, 1990, Sharma et al 2008, Zeybek et al, 2012, Devi et al, 2011, 2014, Cavusoglu et al, 2013 on the effects of plant growth regulators have been done in in vitro.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Exogenously Applied Plant Growth Regulators on Plant Development of Saffron (Crocus sativus L.)

Çavuşoğlu¹

2017

jist

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Plant growth regulators have important roles in plant growth and development. The externally applicable chemicals belong to different action classes and each one has a crucial and effective role at different plant growing stages. Saffron (Crocus sativus L.) is one of the most important and valuable medicinal and aromatic plant, belongs to Iridaceae family. Because of impossibility of generative propagation in nature, growing can be maintened asexually with daughter corms occured each year. In this experiment, the effects of exogenously applied paclobutrazol, indole-3-butyric acid, zeatin and picloram on saffron developmental stages were studied. Data were focused on aerial part and corm related characters which is important in developmental findings in this plant. In the treatments, paclobutrazol (10 or 20 mg L -1 ), indole-3-butyric acid (1 or 3 mg L -1 ), zeatin (1 or 3 mg L -1 ) and picloram (5 or 10 mg L -1 ) were applied alone besides control to saffron 45 days after planting to the soil under greenhouse condition. According to the data picloram concentrations have a retardant role in nodium activation, leaf number and corm developmental characters (number, weight, diameter and yield of daughter corm) but cause the highest plant height (60.98 cm in 10 mg L -1 and 57.37 cm in 5 mg L -1 ). In 10 mg L -1 paclobutrazol treatments resulted as the best corm production (469 kg da -1 ) than the other treatments and control. Zeatin at 3 mg L -1 concentration was found effective on the best nodium activation (2.81 active nodes/corm). In conclusion, saffron development is affected significantly by externally applied plant growth regulators under soil condition and the application methods can be progressed in studies on aimed parts of the plant. Keywords:Crocus sativus, indole-3-butyric acid, paclobutrazol, picloram, zeatin ÖZET: Dışsal uygulanabilen kimyasallar olan ve farklı etki sınıfları içinde bulunan bitki gelişim düzenleyicilerinin her biri bitki büyüme ve gelişiminin farklı aşamaları üzerinde hayati ve önemli işlevlere sahiptir. Tıbbi ve aromatik bitkiler içinde en önemli ve değerli bitkilerden biri olan safran (Crocus sativus L.) Iridaceae familyasına ait olup, doğal olarak generatif yolla üretilmesi mümkün olmadığından bitki gelişimi aseksüel olarak her yıl meydana gelen yavru kormlardan sağlanmaktadır. Bu araştırmada dışsal olarak uygulanan paclobutrazol, indole-3-butyric acid, zeatin and picloram maddelerinin safran gelişimi üzerine etkileri çalışılmıştır. Bitki gelişimi için önemli parametreler olan korm ve toprak üstü aksamı ile ilişkili karakterlerle ilgili veriler toplanmıştır. Çalışmada kontrol parsellerinin yanında paclobutrazol, indole-3-butyric acid, zeatin ve picloram bitki dikiminden 45 gün sonra cam serada toprağa uygulanmıştır. Elde edilen verilere göre picloram konsantrasyonları nodyum aktivasyonu, yaprak sayısı ve korm gelişim özellikleri (korm sayısı, ağırlığı, çapı ve yavru korm verimi) için geciktirici bir rol oynarken, en yüksek bitki boyu (10 mg L -1 de 60.98 cm ve 5 mg L -1 de ...

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“…Direct and indirect organogenesis was reported by Zeybek et al in 2012 using MS medium supplemented with various plant growth regulators [13]. Subsequently, another group reported cormlet production via somatic embryogenesis, using MS medium containing TDZ and picloram [14].…”

Section: Callus Production From Corm Slices In Vitro and Regenerationmentioning

confidence: 94%

Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

et al. 2020

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Background: Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and vegetative reproduction. Effective genome editing requires proficient callus production and an efficient method to deliver Cas9 and sgRNAs into the plant. Here, we demonstrate Agrobacterium-mediated transformation of saffron. Further, we developed a CRISPR-Cas9 based system in this plant, for efficient gene knockout or edits in future. Results: Efficient callus production and regeneration confers important benefits in developing competent transformation system in plants. More than 70% multiplication rate of callus initiation was achieved from corm slices of saffron subjected to a two-step sterilization procedure and grown on complete MS medium supplemented with 2,4-D (0.5 mg/L), BAP (1 mg/L), IAA (1 mg/L), photoperiod of 16/8 h and 45% relative humidity at 20 ± 2 °C. In vitro cormlet generation was accomplished in 8 weeks by using mature somatic embryos on MS medium supplemented with TDZ (0.5 mg/L) + IAA (1 mg/L) + Activated charcoal (0.1 g/L) at 15 ± 2 °C. The attempt of using Agrobacterium-mediated transformation resulted in successful integration of the binary vector into the somatic embryos of saffron with a transformation efficiency of 4%. PCR and Southern blot analysis confirmed the integration of Cas9 into saffron. Conclusion: The protocol for callus production, somatic embryogenesis and regeneration was standardised. Successful demonstration of integrated Cas9 in this study constitutes first step in developing strategies for genetic manipulation of saffron, which has so far been considered recalcitrant. Furthering the development of this technology holds significant potential for advancing genetic research in saffron by integrating multigene targeting and/or use of recyclable cassettes.

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In vitro cormlet production and growth evaluation under greenhouse conditions in saffron (Crocus sativus L.) – A commercially important crop

Cited by 31 publications

References 14 publications

In vitro cormlet production of saffron (Crocus sativus L. Kashmirianus) and their flowering response under greenhouse

In vitro cormlet production of saffron (Crocus sativus L. Kashmirianus) and their flowering response under greenhouse

The Effect of Exogenously Applied Plant Growth Regulators on Plant Development of Saffron (Crocus sativus L.)

Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

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