In vitro conservation of Poincianella pyramidalis (Tul.) L.P. Queiroz under minimal growth conditions

Abstract

Poincianella pyramidalis (Tul.) L.P. Queiroz, is an arboreal species endemic to Brazil’s Caatinga biome and an important source of lumber and also of medicinal substances. This study aimed to evaluate the effect of the osmotic agents sucrose, sorbitol and mannitol and the growth retardant paclobutrazol (PBZ) on the induction of slow growth in vitro of P. pyramidalis, seeking to establish alternative techniques for ex situ conservation of the species. In the first experiment, plantlets with seven days of age were inoculated on Woody Plant Medium (WPM) supplemented with four concentrations of sucrose (87.64, 131.46, 175.28 or 219.10 mM) combined with 0.0 or 87.64 mM sorbitol or mannitol. In the second experiment, these plantlets were inoculated on WPM medium with 0.0, 1.5, 3.0, 4.5 or 6.0 µM PBZ and supplemented with 87.64 mM sucrose. For both experiments, the survival percentage of the plants was evaluated every 60 days, and at the end of 240 days, the following parameters were recorded: number of green leaves and senescent leaves; length of the aerial part and longest root; and dry mass of the aerial part and roots. The best results to maintain the quality of the P. pyramidalis plants conserved in vitro were obtained on medium supplemented with 219.10 mM sucrose for up to 240 days without subculture. With respect to PBZ, the concentration of 6.0 μM can be recommended for in vitro conservation of P. pyramidalis for 240 days without the need of subculturing.

Temperature and GA3 on ROS and cytogenetic stability during in vitro cultivation of strelitzia zygotic embryos

“…The use of growth regulators is beneficial to the in vitro plant growth and development (Ayub et al 2019;Mitrofanova et al 2019;Silva et al, 2019), but it may lead to negative changes in cytogenetic stability (Samarfard et al, 2014, Masouleh;Sassine, 2020). Flow cytometry analyses quantify the DNA content and estimate the ploidy level, since the size of the genome is highly correlated with the number of chromosomes.…”

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

“…Variables analyzed in the present work, such as umber of green leaves, shoot height, number of microcuttings and number of senescent leaves show a good plant development. This last variable is fundamental to aid delimiting the cultivation period and to detect the need for transferring plants to a new culture medium (Silva et al 2019), which directly interferes on labor costs and the resources used to preserve conserved plants.…”

Effects of different doses of paclobutrazol and sucrose in the minimum in vitro growth of wild species of Manihot

“…), obtained positive responses regarding the retardation of plant material development. Silva et al (2019) also reported satisfactory results on in vitro conservation of "catingueira" (Poincianella pyramidalis), recommending using culture media supplemented with SUC and PBZ for up to 240 days, without subculture.…”

“…In this same context, Silva et al (2019), when using PBZ on in vitro conservation of "catingueira" (Poincianella Table 1. Summary of the analysis of variance for plant height (PH, in cm), number of mini-cuttings (NM), number of roots (NR), number of shoots (NS), number of green leaves (NGL) and number of senescent leaves (NSL) for M. pseudoglaziovii, as a function of the concentrations of paclobutrazol (PBZ) and sucrose (SAC).…”