In vitro conservation of mangaba native to Brazilian Cerrado

Abstract

Mangaba (Hancornia speciosa var. gardneri) is a fruit tree of great social, economic and cultural importance in Brazil. Currently, it is threatened with extinction, so the Brazilian Ministry of the Environment has been encouraging the development of studies that can result in conservation strategies for this genetic resource. Thus, the objective of this work was to accomplish the slow-growth in vitro conservation of nodal segments of mangaba native to Brazilian Cerrado by changes in temperature and culture medium, and after that, to conduct the growth recovery of the explants. In the in vitro conservation stage, two experiments were made to test the temperatures of 25 and 20 °C, in which mangaba nodal segments were inoculated in transparent glass jars containing MS or WPM media at full or half strength, being evaluated at 45 and 90 days of in vitro conservation. In order to verify the growth recovery of the explants, these were transferred to multiplication medium, and evaluated at 60 days. Explants conserved at 20 °C showed small and chlorotic leaves compared to those conserved at 25 °C. The reduction of the conservation temperature provided less development of the explants without affecting the survival rate. Explants conserved in WPM medium showed better responses to growth recovery. The results show that mangaba nodal segments conserved for 90 days on half-strength WPM medium and temperature of 20 °C presented slower growth and a good response to growth recovery stage.

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

“…These results reveal the need for further studies related to in vitro nutrition, such as evaluation of culture media and concentrations of their components. As an alternative to the MS medium, the use of the WPM culture medium can show good results in Brazilian native species such as: Hancornia speciosa and Dalbergia nigra (Pires et al, 2019;Pessanha et al, 2022) In general, based on the results obtained in the present study, further investigations in the micropropagation of A. urundeuva are recommended, especially with modifications of the culture medium, as found for Rubus sp. and Eucalyptus Globulus Labill.…”

Advances and perspectives in the propagation of mangabeira (Hancornia speciosa Gomes) - review on a tropical fruit tree with socioeconomic importance

“…• The best results for plant conservation are obtained by adding 30 g L -1 of sucrose to the cultivation medium and carrying out in vitro conservation for up to 90 days. (Santos et al, 2011;Santos et al, 2016;Pires et al, 2020;Oliveira & Aloufa, 2022;Pires et al, 2022) Cryopreservation…”

In vitro culture of Stryphnodendron adstringens Mart. Coville

“…Allemão ex Benth., where both culture media tested showed no significant difference, however, the seedlings of the WPM culture medium presented well-developed leaves, while those obtained from MS culture medium showed more leaf senescence. The WPM medium was also more suitable for the development of Hancornia speciosa Gomes explants (PIRES et al, 2020). According to Hazubska-Przybył (2019) and Phillips and Garda (2019) the concentration of nitrate and ammonium ions in WPM culture medium (25% of the concentration present in MS culture medium), in addition to more potassium and a high level of sulfate ions, are factors that contribute to the development of woody species.…”