In vitro conservation of Valeriana officinalis L. through minimal growth

Abstract

Minimal growth storage could be utilized for in vitro plant conservation. Modifying the culture medium or the environmental condition can reduce the plant growth. The aim of the experiment is to observe the effect of macronutrients on the reduction of valerian culture This research was conducted at Tissue Culture Laboratory at the Indonesian Crops and Medicinal Research Institute (ISMCRI). Single axillary buds with leaves of valerian were used as explants. The treatment applied were :1) full-MS medium + 0.1 mg l-1 BAP, 2) ¾ MS + 0.1 mg l-1 BAP, 3) ½ MS + 0.1 mg l-1 BAP and, 4) ¼ MS + 0.1 mg l-1 BAP. After 12 weeks of conservation, the cultures were transferred into a plant growth regulator free-MS medium to observe their regeneration ability after the conservation. Then, the cultures were acclimatized at the greenhouse to observe their further growth. The result showed that using ¼ MS + 0.1 mg l-1 BAP was the best treatment to reduce the growth during 12 weeks of conservation. After in vitro storage, the cultures can grow normally in full strength plant growth regulator free-MS medium, which was characterized by the formation of new shoots. Valerian also produced roots in the same medium. All acclimatized plantlets were successfully established at the greenhouse.

Composition of media for in vitro slow growth storage (sgs) of Aglaonema

“…MS media enriched with PGR cytokinin and auxin in the right combination will encourage shoot initiation or multiplication of Aglaonema explants (Barakat and Gaber, 2018;Kaviani et al, 2019). On the other hand, the combination of diluting primary media with growth-inhibiting substances (Syahid, 2021;Syahid and Parlindung, 2023). In this study, treatment of reducing the concentration of MS media with the addition of BAP and IAA resulted in slow growth of Aglaonema culture.…”

“…Reducing nutrients in the MS planting medium by reducing the concentration or dilution to 25% (1/4 MS) causes the plantlets to grow less optimally, according to modification of the planting medium by reducing the sucrose level and the concentration of the basic media. Reducing the concentration of basic media with PGR combinations is one of the slowgrowth storage techniques (Syahid and Parlindung, 2023). The number of roots in the P0 treatment (full MS without PGR) showed promising results (1.89 strands).…”

“…In vitro storage using the Slow Growth Storage technique is carried out in several ways, including the use of growth retardant and osmotic compounds (Dewi et al, 2020;Syahid, 2020), encapsulation (Hassanen, 2021), storage in dark conditions and control of the light spectrum (Rodrigues et al, 2022), modification of the growing media by reducing sucrose levels and concentration of primary media , a combination of diluting media with growth inhibitory substances (Syahid, 2021;Syahid and Parlindung, 2023) and the use of a specific light spectrum (Rodrigues et al, 2022). Modifying the media composition by reducing the content of sugars, minerals, growth regulators, or osmotic agents such as sorbitol and mannitol can inhibit cell division and significantly limit callus formation and shoot development.…”