In Vitro Conservation and Cryopreservation of Ornamental Plants

Özüdoğru, Elif Aylin; Previati, A.; Lambardi, Maurizio

doi:10.1007/978-1-60327-114-1_28

Cited by 31 publications

(14 citation statements)

References 41 publications

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“…The improvement of storage techniques is necessary for successful bud regrowth and synthetic seed conversion, including of elite clonal germplasm, endangered cultivars [39,40], and storage of cultivar or species in commercial laboratories. Recently, slow growth storage has found applications in in vitro conservation of several ornamental species [41].…”

Section: Medium-term In Vitro Conservationmentioning

confidence: 99%

An improved encapsulation protocol for regrowth and conservation of four ornamental species

2017

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The encapsulation technology, initially developed for clonal propagation through the production of synthetic seeds with somatic embryos, is currently proposed for use with non-embryogenic explants, such as buds and nodal segments (unipolar propagules). In the present study, the encapsulation procedure and its effect on shoot regeneration were evaluated. Apical buds isolated from shoot cultures of four ornamental species (Photinia × fraseri Dress., Polygala myrtifolia L., Metrosideros excelsa Soland. ex Gaertn., and Rosa) were encapsulated in 3% sodium alginate. Effects of complexation time, sucrose concentration, and storage temperature on the regrowth ability of propagules were assessed. With the appropriate combination of sucrose concentration and polymerization time, the encapsulated explants proved to have a better regrowth (80-100%) after sowing than the naked ones. In addition, medium-term storage of Metrosideros encapsulated explants promoted a high level of regrowth (74%) after 4 months in the dark at 10°C; while polygala beads were preserved up to 8 months regardless of storage temperatures. Potential current applications of encapsulation technology and the future use of beads in vivo conditions are also discussed.

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Section: Medium-term In Vitro Conservationmentioning

confidence: 99%

An improved encapsulation protocol for regrowth and conservation of four ornamental species

2017

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“…Besides their role in the ecological importance, these climbers are also very important in ornamental and medical uses. Ozudogru et al ( 2010 ) reported a review on slow-growth conservation and cryopreservation of ornamental species which refers to the in vitro conservation of shoot cultures by minimising their periodic subcultures without affecting the viability and revival of shoot cultures. Cryopreservation refers to the storage of explants at ultralow temperature of −196 °C, at which all biological activities within the cells are hampered.…”

Section: Introductionmentioning

confidence: 99%

Genetic Transformation for Quality Improvement in Ornamental Climbers

Singh

Srivastava

Misra

2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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The climbers have very important role as ornamentals (e.g. Bougainvillea , Clematis , Bignonia , Lonicera , fl oribunda rose, Asparagus racemosus , Gloriosa superba , Trachelospermum jasminoides , Ceropegia spp., etc.) and as medicinal plants (e.g. Gymnema sylvestre , Tinospora cordifolia , Gloriosa superba , Asparagus racemosus , Piper betle , Holostemma ada-kodien ). But there are many problems associated with their propagation, breeding, untimely ripening of male and female fl owers, biotic and abiotic stress and also many types of diseases. Plant transformations including Agrobacterium -mediated; virus-mediated, chemical-mediated microinjection; electroporation; particle bombardment; RNAi and miRNA technologies are routinely being used for the improvement of crops, to develop desired transgenics and to introduce various types of biotic and abiotic resistance in plants. Mutation breeding is a conventional and time-consuming technique for modifying the target traits in plant systems. To overcome this, genetic transformation can be used for the modifi cation of trait-related genes in few plants.Flower colour, fl ower fragrance, dwarfi sm and increase in vase life of climbing ornamentals are some of the important areas of research which contribute to the market value of any ornamental plant. In last few decades, the relationship between fl avonoid biosynthetic pathway and anthocyanin production has been well studied, and genetic engineering is being used to produce cultivars with novel pigmentation in fl owers by modifi cation in the biosynthesis of fl avonoid/anthocyanin. Initially, blue rose was developed by introducing genes for delphinidin pigments to ordinary roses. But recently, gene silencing using RNAi technology is being used to prevent red pigment formation and allow the production of blue pigment. Colour intensity and fl ower longevity can be increased by the transformation and modulation of genes that encode for pigment and ethylene biosynthetic pathways. However, with the knowledge of genes related to fragrance biosynthesis, one can improve the ornamental climbers, such as Quisqualis indica ,

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“…Sorbitol at 0.1 M concentration was found to be effective in decreasing growth in Teucrium polium L. and Acheillea frarantissima respectively as reported by Rabba'a et al (2012) and Younis (2012). The use of low temperature from 4 to 18 °C and nutrient medium supplementation osmo-regulators with sucrose have been used to conserve explants for clonal propagation such as tubers, roots and ornamental species (Ozudogru et al, 2010). The low temperature increases the transfer interval to new medium and often regardless of the type and concentration of carbohydrate used (Lima-Brito et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Storage of Paulownia Tomentosa

El-Homosany

El-Deen

2019

Scient. J. of Flowers and Ornament. Plants

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Storage of plant species with high commercial potential as ornamental or for the alimentation and pharmacological has been used to preserve genetic variability and allowed the study of its properties in a controlled environment through the use of in vitro culture techniques. In this study, shoot tip explants of Paulownia tomentosa were excised from in vitro grown shootlets and cultured on Murashige and Skoog (MS) medium supplemented with 30 g l -1 sucrose (control) and different concentrations of sucrose or sorbitol (20, 40, 50 and 60 g l -1 ) and in combinations (10 + 10, 20 + 20, 25 + 25 and 30 + 30 g l -1 ) and incubated under 18 °C in complete darkness for 2, 4, 6, 8 and 10 months. Results showed that all shoot tip explants conserved on MS medium augmented with different concentrations of sucrose or sorbitol and their combinations for 2 months, at the level of 30 g l -1 sucrose (control) for 4 months recorded the best survival percentage (100%). At the end of storage period (after 10 months), the highest survival percentage (83.33%) was noticed when explants were conserved on MS medium with 30 g l -1 (control) or 50 g l -1 sucrose. Meanwhile, the lowest survival percentage (25.00%) was achieved when explants were conserved on MS medium with 10 g l -1 sucrose + 10 g l -1 sorbitol. Moreover, it was observed an increasing of shoot length and number of proliferated shoots/explant during storage periods except all treatments with sorbitol and the treatment with sucrose plus sorbitol (10 + 10 g 1 -1 ) remained without change in all storage periods.

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In Vitro Conservation and Cryopreservation of Ornamental Plants

Cited by 31 publications

References 41 publications

An improved encapsulation protocol for regrowth and conservation of four ornamental species

An improved encapsulation protocol for regrowth and conservation of four ornamental species

Genetic Transformation for Quality Improvement in Ornamental Climbers

In Vitro Storage of Paulownia Tomentosa

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