Genetic Transformation for Quality Improvement in Ornamental Climbers

Singh, Gaurav; Srivastava, Mrinalini; Misra, Pratibha

doi:10.1007/978-3-319-19288-8_14

Cited by 3 publications

(3 citation statements)

References 66 publications

(56 reference statements)

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“…Genome sequence information on important decorative plant species would be immensely beneficial in ornamental plant breeding and research following the debut of next-generation sequencing (NGS) technologies in 2005. Genetic transformation is an essential requirement for the development and improvement of flowering crops when distinguished with mutation breeding, which delivers a “subtractive” one-point improvement in flowers [ 18 ]. Ever since the very first transformation in ornamentals described in 1987, genetic transformation is acknowledged as an important method to develop desirable cultivars [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

et al. 2022

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Demand of flowers is increasing with time worldwide. Floriculture has become one of the most important commercial trades in agriculture. Although traditional breeding methods like hybridization and mutation breeding have contributed significantly to the development of important flower varieties, flower production and quality of flowers can be significantly improved by employing modern breeding approaches. Novel traits of significance have interest to consumers and producers, such as fragrance, new floral color, change in floral architecture and morphology, vase life, aroma, and resistance to biotic and abiotic stresses, have been introduced by genetic manipulation. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has recently emerged as a powerful genome-editing tool for accurately changing DNA sequences at specific locations. It provides excellent means of genetically improving floricultural crops. CRISPR/Cas system has been utilized in gene editing in horticultural cops. There are few reports on the utilization of the CRISPR/Cas9 system in flowers. The current review summarizes the research work done by employing the CRISPR/Cas9 system in floricultural crops including improvement in flowering traits such as color modification, prolonging the shelf life of flowers, flower initiation, and development, changes in color of ornamental foliage by genome editing. CRISPR/Cas9 gene editing could be useful in developing novel cultivars with higher fragrance and enhanced essential oil and many other useful traits. The present review also highlights the basic mechanism and key components involved in the CRISPR/Cas9 system.

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Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

et al. 2022

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“…Conventional breeding strategies, such as hybridization and mutant breeding, result in the complicated inheritance of genetic factors and require much time ( Azadi et al, 2016 ). In contrast, genetically modified breeding (GMB) simplifies and expedites breeding by introducing one or more genes to an organism’s genome using contemporary molecular techniques ( Singh et al, 2015 ). In addition, the implementation of GMB relies on a stable and effective genetic transformation system.…”

Section: Introductionmentioning

confidence: 99%

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

et al. 2022

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Calla lily (Zantedeschia spp.) have great aesthetic value due to their spathe-like appearance and richness of coloration. However, embryonic callus regeneration is absent from its current regeneration mechanism. As a result, constructing an adequate and stable genetic transformation system is hampered, severely hindering breeding efforts. In this research, the callus induction effectiveness of calla lily seed embryos of various maturities was evaluated. The findings indicated that mature seed embryos were more suitable for in vitro regeneration. Using orthogonal design experiments, the primary elements influencing in vitro regeneration, such as plant growth regulators, genotypes, and nanoscale materials, which was emergent uses for in vitro regeneration, were investigated. The findings indicated that MS supplemented with 6-BA 2 mg/L and NAA 0.1 mg/L was the optimal medium for callus induction (CIM); the germination medium (GM) was MS supplemented with 6-BA 2 mg/L NAA 0.2 mg/L and 1 mg/L CNTs, and the rooting medium (RM) was MS supplemented with 6-BA 2 mg/L NAA 0.7 mg/L and 2 mg/L CNTs. This allowed us to verify, in principle, that the Agrobacterium tumefaciens-mediated genetic transformation system operates under optimal circumstances using the GUS reporter gene. Here, we developed a seed embryo-based genetic transformation regeneration system, which set the stage for future attempts to create new calla lily varieties.

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“…Given the increasing number of ornamental plant genomes that have been sequenced, the information will be of great help in the breeding of ornamental plants, as well as for basic research. Genetic transformation is a powerful tool and could be useful in producing an "additive" one-point improvement compared to mutation breeding, which produces a "subtractive" one-point improvement [19]. Since the first report of genetic transformation in ornamental plants in 1987 [20], genetic transformation has been recognized as an important technique for generating new cultivars.…”

Section: Introduction Of Genome-editing Technologiesmentioning

confidence: 99%

Progress and Challenges in the Improvement of Ornamental Plants by Genome Editing

Ahn

Ramya

et al. 2020

Plants

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Biotechnological approaches have been used to modify the floral color, size, and fragrance of ornamental plants, as well as to increase disease resistance and vase life. Together with the advancement of whole genome sequencing technologies, new plant breeding techniques have rapidly emerged in recent years. Compared to the early versions of gene editing tools, such as meganucleases (MNs), zinc fingers (ZFNs), and transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeat (CRISPR) is capable of altering a genome more efficiently and with higher accuracy. Most recently, new CRISPR systems, including base editors and prime editors, confer reduced off-target activity with improved DNA specificity and an expanded targeting scope. However, there are still controversial issues worldwide for the recognition of genome-edited plants, including whether genome-edited plants are genetically modified organisms and require a safety evaluation process. In the current review, we briefly summarize the current progress in gene editing systems and also introduce successful/representative cases of the CRISPR system application for the improvement of ornamental plants with desirable traits. Furthermore, potential challenges and future prospects in the use of genome-editing tools for ornamental plants are also discussed.

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Genetic Transformation for Quality Improvement in Ornamental Climbers

Cited by 3 publications

References 66 publications

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

CRISPR/Cas9 System: A Potential Tool for Genetic Improvement in Floricultural Crops

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

Progress and Challenges in the Improvement of Ornamental Plants by Genome Editing

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