In vitro conjugal transfer of tetracycline resistance from<i>Lactobacillus</i>isolates to other Gram-positive bacteria

Gevers, Dirk; Huys, Geert; Swings, Jean

doi:10.1016/s0378-1097(03)00505-6

Cited by 160 publications

(103 citation statements)

References 28 publications

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“…lactis K214 (34), and from raw meat-based fermented products, such as L. alimentarius, L. curvatus, L. plantarum, and L. sakei (19). The Tc r has been found to be mediated mainly by tet(M), which could be plasmid encoded and transferred through interspecies and intergenus conjugation mechanisms (17,27). In this study, we show that L. sakei Rits 9, a Tc r strain isolated from a dairy product, harbors two Tc r genes, namely, the ribosomal protection tet(M) gene frequently encountered in lactobacilli and the efflux pump-encoding tet(L) gene.…”

Section: Discussionmentioning

confidence: 99%

“…However, in recent years a number of studies have correlated atypically high phenotypic resistances with the presence of tet genes (11,17,18,19,20,26). Tetracycline resistance in Lactobacillus has commonly been associated with the presence of tet(M) (19,20), but recently the gene coding for the efflux transporter Tet L was also described for some cloacal isolates (5).…”

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confidence: 99%

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Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Ammor

Gueimonde

Danielsen

et al. 2008

Appl Environ Microbiol

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Lactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. sakei Rits 9, a strain isolated from Italian Sola cheese made from raw milk, is mediated by a transposon-associated tet(M) gene coding for a ribosomal protection protein and a plasmidcarried tet(L) gene coding for a tetracycline efflux pump. pLS55, the 5-kb plasmid carrying the tet(L) gene, is highly similar to the pMA67 plasmid recently described for Paenibacillus larvae, a species pathogenic to honeybees. pLS55 could be transferred by electroporation into the laboratory strain L. sakei 23K. While the L. sakei 23K transformant containing pLS55 displayed an intermediate tetracycline resistance level (MIC, <32 g/ml), L. sakei Rits 9, containing both tetracycline-resistant determinants, had a MIC of <256 g/ml, suggesting that Tet L and Tet M confer different levels of resistance in L. sakei. Remarkably, in the absence of tetracycline, a basal expression of both genes was detected for L. sakei Rits 9. In addition, subinhibitory concentrations of tetracycline affected the expression patterns of tet(M) and tet(L) in different ways: the expression of tet(M) was induced only at high tetracycline concentrations, whereas the expression of tet(L) was up-regulated at lower concentrations. This is the first time that two different mechanisms conferring resistance to tetracycline are characterized for the same strain of a lactic acid bacterium.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Ammor

Gueimonde

Danielsen

et al. 2008

Appl Environ Microbiol

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“…Transfer of tetracycline resistance (i.e. the tet(M) gene) from Lactobacillus isolates originating from fermented dry sausages to E. faecalis and L. lactis was demonstrated in conjugation experiments by Gevers et al [55]. Although the transposon Tn2009 was originally identified in S. pneumoniae as nonmobilizable [20], in a later study, however, its conjugation was proved between a plenty of various bacteria, such as Acinetobacter junii, Citrobacter spp., E. coli, Enterobacter cloacae, Klebsiella spp., Pantoeaagglomerans, Proteus spp., Pseudomonas spp., Ralstoniapickettii,Stenotrophomonasmalto philia, E. faecalis, Neisseria mucosa and Neisseria perflava [56].…”

Section: Transmission Of Tetracycline and Mls Resistancementioning

confidence: 99%

Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage – a review

Marosevic¹,

Kaevska²,

Jaglič³

2017

Ann Agric Environ Med.

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Marosevic D, Kaevska M, Jaglic Z. Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage -a review. Ann Agric Environ Med. 2017; 24(2): 338-344. https://doi.org/10.26444/aaem/74718 AbstractAn excessive use of antimicrobial agents poses a risk for the selection of resistant bacteria. Of particular interest are antibiotics that have large consumption rates in both veterinary and human medicine, such as the tetracyclines and macrolidelincosamide-streptogramin (MLS) group of antibiotics. A high load of these agents increases the risk of transmission of resistant bacteria and/or resistance determinants to humans, leading to a subsequent therapeutic failure. An increasing incidence of bacteria resistant to both tetracyclines and MLS antibiotics has been recently observed. This review summarizes the current knowledge on different tetracycline and MLS resistance genes that can be linked together on transposable elements.

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“…There is evidence to suggest that this same gene cluster may also be present in other histamine producers, such as Lactobacillus 30a and O. oeni 9204. Also, lactobacilli are able to transfer a conjugative plasmid to bacteria of the same or different genera (Gevers et al, 2003). Lucas et al (2005) suggest that a plasmid-encoded HDC system could be transferred horizontally; and that the location of the gene on an unstable plasmid may explain the random distribution of HDC positive bacteria.…”

Section: Histidine Decarboxylasementioning

confidence: 99%

Biogenic Amines in Wine: Understanding the Headache

Smit

Toit

2016

SAJEV

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The presence of biogenic amines in wine is becoming increasingly important to consumers and producers alike, due to the potential threats of toxicity to humans and consequent trade implications. In the scientific field, biogenic amines have the potential to be applied as indicators of food spoilage and/or authenticity. Biogenic amines can be formed from their respective amino acid precursors by various microorganisms present in the wine, at any stage of production, ageing or storage. To understand the large number of factors that could influence the formation of biogenic amines, the chemical, biochemical, enzymatic and genetic properties relating to these compounds have to be considered. Analytical and molecular methods to detect biogenic amines in wine, as well as possibilities that could enable better control over their production levels in wine will also be explored in this review.

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In vitro conjugal transfer of tetracycline resistance fromLactobacillusisolates to other Gram-positive bacteria

Cited by 160 publications

References 28 publications

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet (L) and Chromosomally Located Transposon-Associated tet (M), Coexist in Lactobacillus sakei Rits 9

Resistance to the tetracyclines and macrolide-lincosamide-streptogramin group of antibiotics and its genetic linkage – a review

Biogenic Amines in Wine: Understanding the Headache

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