In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers

Lee, Ju Kyung; Kim, Sunil; Lee, Sukjoon; Kim, Hyeon‐Cheol; Kim, Euiseong

doi:10.3390/ma12152411

Cited by 37 publications

(53 citation statements)

References 27 publications

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“…Unsurprisingly, Bio-C Sealer and TotalFill BC Sealer were significantly higher than AH Plus. These results were in line with those of a previous study that showed that EndoSequence BC, BioRoot RCS, and Endoseal MTA showed a significant increase in mineralized nodule formation compared to the AH Plus group [11]. In addition, a recent study with endodontic sealers showed that TotalFill BC Sealer induced alkaline phosphatase activity and strong mineralization capacity [26].…”

Section: Discussionsupporting

confidence: 90%

“…Bio-C Sealer and TotalFill BC Sealer are bioactive in nature; i.e., they induce mineralization when in contact with biological tissues [20]. AH Plus was used as a control because it is one of the most commonly used and investigated root filling materials [11,21,22].…”

Section: Discussionmentioning

confidence: 99%

“…In this context, stem cells from dental tissues and their interaction with the endodontic materials have been considered an important event that favors biomineralization [7]. Thus, stem cells from periodontal tissues (hPDLSCs) have been previously used as cell models for in vitro cytotoxic studies involving endodontic sealers, because these cells may be in direct contact with sealer extrusions [8,9,10,11]. These cells have colony forming ability, spindle-like cell morphology, specific cell-surface marker expression, multipotent differentiation, and immunomodulatory functions [11].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

et al. 2019

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The aim of this study was to investigate the cytocompatibility and mineralization potential of two premixed hydraulic endodontic sealers compared with an epoxy resin-based root canal sealer. The cellular responses and mineralization capacity were studied in human periodontal ligament stem cells (hPDLSCs) that were exposed to premixed hydraulic sealers, Bio-C Sealer (Angelus, Londrína, PR, Brazil), TotalFill BC Sealer (FKG Dentaire SA, La-Chaux-de-fonds, Switzerland) and an epoxy resin-based material, AH Plus (Dentsply De Trey, Konstanz, Germany). Non-exposed cultures served as the control. The endodontic sealers were assessed using scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). Statistical analyses were done using Analisis of Variance (ANOVA), with Bonferroni adjusted pairwise comparison (p = 0.05). AH Plus reduced cell viability and cell migration, whereas increased cell viability and cell migration were observed in the Bio-C Sealer and the TotalFill BC Sealer (p < 0.05). The lowest cell attachment and spreading were observed for all concentrations of AH Plus, whereas the highest were observed for TotalFill BC Sealer. At the end of 21 days, only the Bio-C Sealer and the TotalFill BC Sealer supported matrix mineralization (p < 0.05). Additionally, SEM-EDX revealed high content of calcium, oxygen, and silicon in the Bio-C Sealer and the TotalFill BC Sealer. Based on the results from this study, Bio-C Sealer and TotalFill BC Sealer demonstrated better cytocompatibility in terms of cell viability, migration, cell morphology, cell attachment, and mineralization capacity than AH Plus.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

et al. 2019

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“…Calcium silicate-based sealers, including more reactive calcium-silicate-based bioactive glass, known as bioactive root canal sealers, have been recently introduced [ 30 , 31 , 32 ]. Bioactive glass has been mainly used for dental hard tissue regeneration; however, it has also recently shown promise for the repair of several complex tissues [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Kim

Lee

et al. 2020

Nanomaterials

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Calcium silicate-based bioactive glass has received significant attention for use in various biomedical applications due to its excellent bioactivity and biocompatibility. However, the bioactivity of calcium silicate nanoparticle-incorporated bioactive dental sealer is not much explored. Herein, three commercially available bioactive root canal sealers (Endoseal MTA (EDS), Well-Root ST (WST), and Nishika Canal Sealer BG (NBG)) were compared with a resin-based control sealer (AH Plus (AHP)) in terms of physical, chemical, and biological properties. EDS and NBG showed 200 to 400 nm and 100 to 200 nm nanoparticle incorporation in the SEM image, respectively, and WST and NBG showed mineral deposition in Hank’s balanced salt solution after 28 days. The flowability and film thickness of all products met the ISO 3107 standard. Water contact angle, linear dimensional changes, and calcium and silicate ion release were significantly different among groups. All bioactive root canal sealers released calcium ions, while NBG released ~10 times more silicon ions than the other bioactive root canal sealers. Under the cytocompatible extraction range, NBG showed prominent cytocompatibility, osteogenecity, and angiogenecity compared to other sealers in vitro. These results indicate that calcium silicate nanoparticle incorporation in dental sealers could be a potential strategy for dental periapical tissue regeneration.

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“…(Shashank et al, 2019) alka¬line pH could neutralize the lactic acid from osteoclasts and prevent dissolution of minera¬lized components of teeth. (poggio et al, 2017) We have numerous highly cited publications on well designed clinical trials and lab studies (Govindaraju, neelakantan and Gutmann, 2017;azeem and Sureshbabu, 2018;Jenarthanan and Subbarao, 2018;manohar and Sharma, 2018;nandakumar and nasim, 2018;Teja, Ramesh and priya, 2018;Janani and Sandhya, 2019;khandelwal and palanivelu, 2019;malli Sureshbabu et al, 2019;poorni, Srinivasan and nivedhitha, 2019;Rajakeerthi and ms, 2019;Rajendran et al, 2019;Ramarao and Sathyanarayanan, 2019;Siddique, nivedhitha and Jacob, 2019). This has provided the right platforms for us to pursue the current study.…”

Section: Dental Communicationmentioning

confidence: 99%

Assessment of pH of Calcium Silicate Based Root Canal Sealers at Various Time Periods – an In Vitro Study

Meenapriya¹

2020

Biosci. Biotech. Res. Comm

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Calcium silicate sealers otherwise known as the bioceramic sealers were introduced into dentistry as an alternative root canal sealer. This material originally came from the combination of calcium silicate and calcium phosphate. Calcium phosphate enhances the setting properties resulting in a chemical composition and crystalline structure similar to tooth and bone apatite materials, thereby improving the sealer to root-dentin bonding. Comparison of the physicochemical and biological properties of bio ceramic sealers with the conventional sealers reveals that the calcium silicate based sealers are similar or better. Therefore, the aim of this study is to assess the pH of calcium silicate based root canal sealer at various time periods. Commercial brand of calcium silicate sealer [Bioroot RCS, Septodont] was used for this study. To determine pH, 3 samples were prepared and introduced into polyethylene tubes. Each sample was placed in a tube containing 10ml of distilled water and stored at 37degree C. pH assessment was performed after 1hr, 3hr, 24hr, 48hr, 120hr, 168hr, 192hrs, 28 days after immersion. The samples were assessed for pH using the digital pH meter. The control for the method was based on the reading of pH values of distilled water in which no samples were immersed. Descriptive analysis of the values was performed. The pH of calcium silicate based root canal sealer at various time periods was 11.1 at 1hr, 11.5 at 3hrs, 11.9 at 24hrs, 11.9 at 48hrs, 12.3 at 120hrs, 12.2 at 168hrs, 12.0 at 192hrs, 11.9 at 28 days. The pH of the distilled water in which no samples were immersed was found to be 7.04. Bioroot RCS calcium silicate based sealer had alkaline pH. High alkaline pH of the sealer assumes particular importance because it enhances the deposition of hard tissue, improving the antibacterial properties by the release of calcium ions. Clinical trials and long term follow up studies would be highly valuable to evaluate the bio ceramic sealer's clinical performance.

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In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers

Cited by 37 publications

References 27 publications

Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Assessment of pH of Calcium Silicate Based Root Canal Sealers at Various Time Periods – an In Vitro Study

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