In vitro clonal propagation of Nyctanthes arbortristis Linn. - a medicinal tree

Abstract

Rapid shoot multiplication of Nyctanthes arbortristis was achieved from axillary meristems on Murashige and Skoog (MS) basal medium supplemented with 1.0-1.5 mg/l 6-benzyladenine (BA), 50 mg/l adenine sulfate (Ads) and 3% (m/v) sucrose. Inclusion of indole-3-acetic acid (IAA) in the culture medium along with BA + Ads promoted a higher rate of shoot multiplication. Maximum mean number of microshoots per explant (6.65) was achieved on the MS medium supplemented with 1.5 mg/l BA, 50 mg/l Ads and 0.1 mg/l IAA after 4 weeks of culture. The elongated shoots rooted within 13 to 14 days on ½ strength MS medium supplemented either with indole-3-butyric acid (IBA), IAA or naphtylacetic acid (NAA) with 2% sucrose. Maximum percentage of rooting was obtained on medium having 0.25 mg/l IBA, 0.1 mg/l IAA and 2% sucrose. About 70% of rooted plantlets survived in the greenhouse. The in vitro raised plants were grown normally in the soil condition. This result will facilitate the conservation and propagation of the important medicinal plant.

Plant regeneration from leaf explants of Aloe barbadensis Mill. and genetic fidelity assessment through DNA markers

“…Indiscriminate over exploitation coupled with insufficient attempts for replenishment of wild stock have contributed to its threatened status requiring scientific efforts for conservation and commercial cultivation. Due to low seed viability, germination rate, limited availability of raw material with high quality and slow vegetative growth, tissue culture technique is an alternate solution for conservation of those valuable medicinal plants (Rout et al 2008;Bantawa et al 2011;Swarna and Ravindhran 2012). Though tissue culture is recognized as one of the key areas of biotechnology for large scale propagation and conservation, but it is severely hindered due to somaclonal variations.…”

Analgesic and Anti-Inflammatory Activities of the Methanolic Stem Bark Extract ofNyctanthes arbor-tristisLinn.

“…Nyctanthes arbor-tristis Linn is a small sacred ornamental tree known across India for its fragrant white flowers [7, 8]. Nyctanthes arbor-tristis Linn is commonly known as Night Jasmine [9, 10].…”

“…The bright orange corolla tubes of the flowers contain a coloring substance nyctanthin, which is identical with ά -Crocetin (C 20 H 24 O 4 ) from Saffron. The corolla tubes were formerly used for dyeing silk, sometimes together with safflower or turmeric [8]. Traditionally, the powdered stem bark is given in rheumatic joint pain, in treatment of malaria and also used as an expectorant [10].…”

“…It is given with little sugar to children as a remedy for intestinal ailments. In several cases, it has been found to act efficaciously for malaria fever [8]. The decoction of leaves is extensively used by Ayurvedic physicians for the treatment of arthritis, obstinate sciatica, malaria, and intestinal worms and as a tonic, cholagogue, and laxative [20].…”

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“…In North Eastern India the plant is known as Maha-tita literally "King of bitters", known as various vermicular names. [1][2][3][4][5][6][7] Annona muricata is a member of the Annonaceae family and is a fruit tree with a long history of traditional use. A. muricata, also known as soursop, graviola and guanabana, is an evergreen plant that is mostly distributed in tropical and subtropical regions of the world.…”