In vitro clonal propagation of an adult tree of neem (Azadirachta indica A. Juss.) by forced axillary branching

Chaturvedi, Rakhi; Razdan, M. K.; Bhojwani, Sant S.

doi:10.1016/j.plantsci.2003.10.021

Cited by 31 publications

(21 citation statements)

References 10 publications

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“…Hence, ½ MS medium was used for establishing primary cultures from nodal segments. Similar to the present observation, low salt concentration was reported to be superior for shoot proliferation in Terminalia arjuna (Pandey et al 2006) and Azadirachta indica (Chaturvedi et al 2004).…”

Section: Resultssupporting

confidence: 91%

“…4f). Optimum rooting response using IBA has been reported for many trees including T. arjuna (Pandey et al 2006), Azadirachta indica (Chaturvedi et al 2004) and T. chebula well-developed shoot and roots were taken out and washed gently to remove traces of agar and transferred to earthen pots containing garden soil and organic manure in 1:1 ratio. These pots were initially covered by polythene bags to maintain humidity and kept at 26±2°C.…”

Section: Resultsmentioning

confidence: 99%

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Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Dangi

Khurana-Kaul

Kothari

et al. 2014

Physiol Mol Biol Plants

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The present study reports an efficient in vitro micropropagation protocol for a medicinally important tree, Terminalia bellerica Roxb. from nodal segments of a 30 years old tree. Nodal segments taken from the mature tree in MarchApril and cultured on half strength MS medium gave the best shoot bud proliferation response. )] in the culture medium aided to minimize browning and improve explant survival during shoot bud induction. Highest multiplication of shoots was achieved on medium supplemented with 6-benzyladenine (BA, 8.8 μM) and α-naphthalene acetic acid (NAA, 2.6 μM) in addition to antioxidants. Shoot elongation was obtained on MS medium containing BA (4.4 μM)+phloroglucinol (PG, 3.9 μM). Elongated shoots were transferred to half strength MS medium containing indole-3-butyric acid (IBA, 2.5 μM) for root development. The acclimatization of plantlets was carried out under greenhouse conditions. The genetic fidelity of the regenerated plants was checked using inter simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) analysis. Comparison of the bands among the regenerants and mother plant confirmed true-to-type clonal plants.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Dangi

Khurana-Kaul

Kothari

et al. 2014

Physiol Mol Biol Plants

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“…To be more specific, mid of April was the best time for explant collection for obtaining maximum bud-break which is about 80% with minimum infection, i.e., 20%. Similar results were obtained by Chaturvedi et al (2004) in neem. This period coincides with optimum new growth in field-grown trees, which was known to be more responsive for bud-break as has also been reported by Dhar and Upreti (1999 (Kumar et al 2005).…”

Section: Resultssupporting

confidence: 89%

“…Many workers have attempted micropropagation of mature neem trees but failed to show either sustained proliferation in prolonged cultures or transfer of in vitro-raised plants in field (Joshi and Thengane 1996;Islam et al 1997;Quraishi et al 2004). However, there is only one report on micropropagation of adult neem tree, more or less, of same maturity through axillary shoot proliferation by Chaturvedi et al (2004), but they reported the transfer of plants only up to potted soil stage.…”

mentioning

confidence: 99%

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

et al. 2009

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An efficient in vitro process for rapid clonal propagation of a 40-year-old tree of Azadirachta indica employing nodal stem segments was developed. Season of collection and maturity of explants showed direct influence on bud-break. Nodal stem segments collected during the month of April gave best response. Maximum bud-break (78.6-81%) was obtained when middle order nodes (3rd or 4th node from apex) were taken. Amongst different cytokinins used, 6-benzylaminopurine (BAP) at the concentration of 1.11 lM was found most effective in inducing multiple shoots, whereas inorganic and organic constituents of the medium influenced growth and general condition of proliferating shoots. On an average 3.1 shoots per explant were regenerated in modified Murashige and Skoog medium supplemented with 1.11 lM BAP, 1.43 lM indole-3-acetic acid (IAA) and 81.43 lM adenine hemisulphate. Isolated shoots were rooted in presence of 2.46 lM indole-3-butryic acid (IBA). Root induction took place in 8-10 days with 100% rooting. The in vitro-raised plantlets were successfully transplanted in potted soil and finally grown under field conditions with 100% survival. The genetic fidelity of such in vitro-raised field-grown plants was ascertained by random amplified polymorphic DNA (RAPD) markers. Furthermore, the azadirachtin content of in vitro-cloned plants was found comparable to the mother tree proving their chemical stability also. The protocol developed holds good for in vitro cloning of mature elite neem trees.

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“…CH (500 mg L −1 ) supplemented medium did not support shoot growth as a consequence of which shoots remained compact and stunted. In some cases reduced concentrations of CH induced elongation of shoots [44] . CH was unable to induce indirect multiple shoot formation from calli as efficiently as directly from the explants (Graph 4).…”

Section: Casein Hydrolysate (Ch)mentioning

confidence: 99%

Effect of Additives on Micropropagation of an Endangered Medicinal Tree Oroxylum indicum L. Vent

Bansal¹,

Gokhale²

2012

Recent Advances in Plant in Vitro Culture

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In vitro clonal propagation of an adult tree of neem (Azadirachta indica A. Juss.) by forced axillary branching

Cited by 31 publications

References 10 publications

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

Effect of Additives on Micropropagation of an Endangered Medicinal Tree Oroxylum indicum L. Vent

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