In vitro Clonal Propagation and Organogenesis in Spilanthes acmella (L) Murray: A Herbal Pesticidal Plant of North-East India

Deka, P. C.; Kalita, Mohan Chandra

doi:10.1007/bf03263230

Cited by 10 publications

(6 citation statements)

References 7 publications

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“…2). This number appears to be the higher as compared to all other previous reports on the same plant species (Saritha et al 2002;Haw and Keng 2003;Deka and Kalita 2005;Saritha and Naidu 2008). In the trials that aimed at investigating the interactive effect of BAP and Kin or NAA, the number of shoots produced per explant was higher in the medium containing 5.0 mg dm -3 BAP alone and inclusion of Kin (1.0-5.0 mg dm -3 ) or NAA (1.0-5.0 mg dm -3 ) in medium did not have positive effects on shoot multiplication.…”

Section: Resultscontrasting

confidence: 56%

“…In order to stop using contaminated pharmaceutical raw materials and fulfill the demands of pharmaceutical industries, it is necessary to develop an efficient method of propagation of S. acmella by a suitable agrotechnology. During past years, considerable efforts have been made for in vitro plant regeneration of this important medicinally important and herbal pesticidal plant through organogenesis (Saritha et al 2002;Haw and Keng 2003;Deka and Kalita 2005;Saritha and Naidu 2008). So, in order to obtain rapid and efficient plant regeneration with a high frequency, the transverse thin cell layer (tTCL) culture method was exploited for mass propagation of S. acmella.…”

Section: Introductionmentioning

confidence: 99%

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An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

et al. 2009

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An efficient and improved in vitro propagation system for Spilanthes acmella L. using transverse thin cell layer (tTCL) culture system was established. The frequency of shoot regeneration from tTCL nodal segments was affected by concentrations of plant growth regulators and orientation of the explant. MS (Murashige and Skoog in Physiol Plant 15:473-497, 1962) medium with 5.0 mg dm -3 BAP was optimal for shoot regeneration. Upon this medium, the explant inoculated in the upright orientation exhibited a high frequency of shoot regeneration (about 97%), and the highest number of shoots (31.5) per explant. The intact node (1.0-1.5 cm) cultured on the same medium had significantly lower shoot multiplication ability with only 4.5 shoots per responsive explant. As compared to BAP alone, the combination of BAP and Kin or NAA did not have positive effects on shoot multiplication from tTCL nodal segments. Rooting of shoots was achieved on growth regulator free full-strength MS medium. Plantlets were transplanted into soil with 90-100% survival rate.

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Section: Resultscontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

et al. 2009

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“…Consequently, liquid medium was used throughout this study. The growth ability of S. oleracea nodal explants was improved with the additional BA source (Figure 1a, b and Table 2) as previously reported (Haw and Keng, 2003;Saritha et al, 2002;Bais et al, 2002;Deka and Kalita, 2005;Saritha and Naidu, 2007). Furthermore, the assayed concentrations (2.22 and 4.44 M) did not show significant differences in the explant responses except in relation to the root development.…”

Section: In Vitro Culture Of S Oleraceasupporting

confidence: 84%

Determination of toxicity and chromatographic analysis of spilanthol content in in vitro culture of Spilanthes oleracea Jacq.

Gesteira¹,

Simas²,

Morgana³

et al. 2018

Afr. J. Biotechnol.

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Spilanthes oleracea Jacq. has been extensively used due to its pharmacological activities, and it has been considered as a promising vegetable crop. Nodal-segment cultures of S. oleracea were established in order to analyze the content of alkamide spilanthol. On average, 11 buds/nodal segment were developed after 30 days on MS basal salts liquid medium containing 2.22 μM benzyladenine (BA). Regenerated shoots formed complete plantlets in medium without growth regulators, and 80.2% of the regenerated plantlets survived in field conditions. Higher production of spilanthol (58.5% relative area) was obtained from the leaves and stems of plants grown in liquid medium up to 90 days, than in fieldgrown plants. Addition of BA to the culture medium significantly affected spilanthol accumulation. The production of spilanthol was also detected on the shoot basal callus. The spilanthol content varied with the plant organ in micropropagated plants. Ethanolic extract of leaves from field-grown plants showed no acute toxicology in Swiss mice at a dosage of 3 g/kg.

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“…During past years, considerable efforts have been made for in vitro plant regeneration of this important plant through organogenesis (Saritha et al 2002;Haw and Keng 2003;Deka and Kalita 2005;Saritha and Naidu 2008). However, there is no report on synthetic seed production using either somatic embryos or vegetative propagules in S. acmella.…”

Section: Introductionmentioning

confidence: 97%

Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species

et al. 2008

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This article demonstrates the plantlet regeneration from alginate-encapsulated shoot tips of Spilanthes acmella. Shoot tip explants excised from in vitro proliferated shoots were encapsulated in calcium alginate beads. The best gel complexation for encapsulation of shoot tips was achieved using 3% sodium alginate and 100 mM calcium chloride. Maximum percent response for the conversion of encapsulated shoot tips into plantlets was obtained on growth regulator-free full-strength liquid MS (Murashige and Skoog, Physiol Plant 15:473-497, 1962) medium. The addition of MS nutrients in alginate matrix was found to have pronounced effect on shoot and root emergence from alginate beads. Encapsulated shoot tips could be stored at low temperature (4°C) up to 60 days. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully. The present synthetic seed technology could be useful in large-scale propagation as well as short-term conservation and germplasm distribution and exchange of Spilanthes acmella.

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In vitro Clonal Propagation and Organogenesis in Spilanthes acmella (L) Murray: A Herbal Pesticidal Plant of North-East India

Cited by 10 publications

References 7 publications

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

Determination of toxicity and chromatographic analysis of spilanthol content in in vitro culture of Spilanthes oleracea Jacq.

Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species

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