Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species

Singh, Shashi Kant; Manoj, K.; Asthana, Pooja; Pandey, Sarita; Jaiswal, Vivek; Jaiswal, Uma

doi:10.1007/s11738-008-0250-7

Cited by 67 publications

(20 citation statements)

References 21 publications

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“…Of the three different medium composition tested, best response for shoot sprouting from encapsulated shoot tips was recorded on M 1 medium (Table 1). However, contrary to some previous reports (Rai et al 2008a;Singh et al 2009;Verma et al 2010), all three medium composition inhibited the rooting in shoots that emerged from encapsulated shoot tips, which was overcome after transfer of sprouted shoots to 0.8 % agar-solidified MS medium containing 1 mg l −1 IBA after 4 weeks. After transfer to IBA containing medium, about 70 % of encapsulated shoot tips were rooted (Fig.…”

Section: Resultscontrasting

confidence: 99%

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Kumar

Manoj

Singh

et al. 2010

Physiol Mol Biol Plants

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Shoot tips excised from in vitro proliferated shoots derived from nodal explants of jojoba [Simmondsia chinensis (Link) Schneider] were encapsulated in calcium alginate beads for germplasm exchange and distribution. A gelling matrix of 3 % sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Best response for shoot sprouting from encapsulated shoot tips was recorded on 0.8 % agarsolidified full-strength MS medium. Rooting was induced upon transfer of sprouted shoots to 0.8 % agar-solidified MS medium containing 1 mg l −1 IBA. About 70 % of encapsulated shoot tips were rooted and converted into plantlets. Plants regenerated from encapsulated shoot tips were acclimatized successfully. The present encapsulation approach could also be applied as an alternative method of propagation of desirable elite genotype of jojoba.

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Section: Resultscontrasting

confidence: 99%

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Kumar

Manoj

Singh

et al. 2010

Physiol Mol Biol Plants

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“…In fact, the maximum regrowth in in vitro conditions was 100% in photinia and rose, and 93% and 80% in metrosideros and polygala, respectively. This could be due to the suitable composition of artificial endosperm to stimulate the regrowth of beads after sowing and it is consistent with previous literature, which reported the sodium alginate matrix provides protection and supplies nutrients [12,31].…”

Section: Effect Of Encapsulation On the Bud Regrowthsupporting

confidence: 91%

An improved encapsulation protocol for regrowth and conservation of four ornamental species

2017

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The encapsulation technology, initially developed for clonal propagation through the production of synthetic seeds with somatic embryos, is currently proposed for use with non-embryogenic explants, such as buds and nodal segments (unipolar propagules). In the present study, the encapsulation procedure and its effect on shoot regeneration were evaluated. Apical buds isolated from shoot cultures of four ornamental species (Photinia × fraseri Dress., Polygala myrtifolia L., Metrosideros excelsa Soland. ex Gaertn., and Rosa) were encapsulated in 3% sodium alginate. Effects of complexation time, sucrose concentration, and storage temperature on the regrowth ability of propagules were assessed. With the appropriate combination of sucrose concentration and polymerization time, the encapsulated explants proved to have a better regrowth (80-100%) after sowing than the naked ones. In addition, medium-term storage of Metrosideros encapsulated explants promoted a high level of regrowth (74%) after 4 months in the dark at 10°C; while polygala beads were preserved up to 8 months regardless of storage temperatures. Potential current applications of encapsulation technology and the future use of beads in vivo conditions are also discussed.

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“…In vitro conservation strategies have several benefits for the quick cultivation of medicinal plants, thereby providing a continuous supply of plant materials from elite germplasm lines, which can make significant contributions to the exploitation of therapeutic properties of these plant species and eliminate the need for harvest from the wild. Recently, there has been an increased interest in using in vitro culture techniques for medicinal plant regeneration from various explant sources (Balaraju et al 2009;Baskaran and Jayabalan 2008;Singh et al 2009;Thomas and Shankar 2009). Regarding the tissue culture work on related taxa, Nath and Buragohain (2005) standardized a micropropagation protocol for Adhatoda vasica.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus

Thomas

Hoshino

2010

Acta Physiol Plant

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Justicia gendarussa is a valuable medicinal plant and various parts of this plant are pharmaceutically used for the treatment of different diseases. In vitro regeneration of shoot buds was obtained from culture of nodal cuttings as well as shoot regeneration from callus. The nodal cuttings differed in shoot proliferation in terms of percentage of explants that responded and average shoot length with various concentrations (4.4, 8.9, 13.3, 17.7, 22.2 lM) of 6-benzyladenine (BA), kinetin (Kn) and thidiazuron. In all treatments, one shoot was invariably present. Optimum 87% of cultures responded with an average shoot length of 4.4 cm on Murashige and Skoog (MS) medium supplemented with 17.7 lM BA. Callus was induced from the mature leaf segments on MS medium supplemented with Kn (4.7, 13.9, 23.2 lM) alone or in combination with 2, 4-dichlorophenoxyacetic acid (2, 4-D; 2.3 lM, 4.5 lM). Optimum callus induction (78%) was obtained on MS medium supplemented with 14 lM Kn and 4.5 lM 2, 4-D. When the callus was subcultured on MS medium fortified with BA (8.9, 17.7, 26.6 lM) or Kn (9.3, 18.6, 27.9 lM) alone or in combination with a naphthalene acetic acid (NAA; 2.7, 5.4 lM), shoot regeneration was obtained. The highest response (92%) was observed on MS medium containing 17.7 lM BA and 5.4 lM NAA. On this medium, an average number of 12.2 shoots were obtained per responding callus. The shoots obtained from callus and nodal cuttings were rooted with a frequency of 73% on MS medium augmented with 9.8 lM indole-3-butyric acid. The rooted shoots were successfully transplanted to soil and sand mixture (1:1) with 90% survival rate. The protocol standardized for shoot proliferation and regeneration in J. gendarussa from nodal cuttings and leaf-derived callus is suitable for micropropagation and conservation of this essential medicinal plant.

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Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species

Cited by 67 publications

References 21 publications

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

An improved encapsulation protocol for regrowth and conservation of four ornamental species

In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus

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