In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects

Abstract

Phlorotannins are polyphenols occurring exclusively in some species of brown algae, known for numerous biological activities, e.g., antioxidant, antiproliferative, antidiabetic, and antiallergic properties. Their effects on the response of human lung cells to benzo[a]pyrene (B[a]P) has not been characterized. Our objective was to in vitro evaluate the effects of a phlorotannin-rich extract obtained from the brown algae Ascophyllum nodosum and Fucus vesiculosus on B[a]P cytotoxic effects. The A549 cell line was incubated with B[a]P for 48 and 72 h in the presence or absence of the brown algae extract. Cytochrome P450 activity, activation of P2X7 receptor, F-actin disorganization, and loss of E-cadherin expression were assessed using microplate cytometry and fluorescence microscopy. Relative to control, incubation with the brown algae extract was associated with lower B[a]P-induced CYP1 activity, lower P2X7 receptor activation, and lower reactive oxygen species production. The brown algae extract inhibited the alterations of F-actin arrangement and the downregulation of E-cadherin expression. We identified a phlorotannins-rich extract that could be deeper investigated as a cancer chemopreventive agent to block B[a]P-mediated carcinogenesis.

Pregnant Women and Endocrine Disruptors: Role of P2X7 Receptor and Mitochondrial Alterations in Placental Cell Disorders

“…The three cell lines were selected for their different endocrine properties and because they all express functional P2X7 receptors [13,26,34]. Human epithelial pulmonary A549 cells share similar ultrastructural characteristics and cytochromes expression to in situ type II pneumocytes, the most abundant cells in lungs.…”

“…Cell incubation: To study the P2X7 receptor and its relationship between apoptosis, the cells were preincubated with either PBS or P2X7 antagonist Brilliant Blue G (BBG) at 25 µM for 15 min [34]. After removal of PBS and BBG, the cells were incubated for 72 h with bisphenol A (5, 10 and 20 µM), diethylstilbestrol (3.75, 7.5 and 15 µM), 4-tert-amylphenol (1, 10 and 50 µM), 4-heptylphenol (1, 10 and 50 µM), triclosan (0.1, 1 and 10 µM), propylparaben (20, 50 and 100 µM), benzyl butyl phthalate (1, 10 and 50 µM), DEHP (1, 10 and 50 µM) and 3-benzylidene camphor (1, 10 and 50 µM) in MEM with 2.5% FBS according to Olivier et al's protocol that describes the JEG-Tox model [35] or DMEM with 2.5% FBS for the HaCaT and A549.…”

Phlorotannins–bioactivity and extraction perspectives

“…Dutot et al ( 2021 ) studied the chemopreventive potential of phlorotannins based on a benzopyrene induced cytotoxicity study. It was observed that phlorotannin administration helped in counteracting the cytotoxic affects induced by benzopyrene through several pathways such as by preventing the induction of cytochrome P450 activity by benzopyrene, by inhibiting the activation of P2X7 receptor whose activation is reported to induce anti-apoptotic effects, inhibiting actin rearrangement and the production of reactive oxygen species.…”

The Impact of Natural Deep Eutectic Solvents and Extraction Method on the Co-Extraction of Trace Metals from Fucus vesiculosus

“…The brown seaweed Fucus vesiculosus attracts the attention of specialists as a rich source of polysaccharides, phlorotannins, carotenoids, and other active compounds, which shows potent activities as antioxidants [ 16 ], antiangiogenic [ 17 ], anti-cancer [ 18 , 19 , 20 ], inhibitors of diabetes controlling enzymes [ 21 , 22 ], antimicrobial [ 23 ], anti-inflammatory [ 22 , 24 ], anti-coagulant [ 22 ], skin brightening [ 25 ], etc.…”