In Vitro Characterization of Four N-[4-(5-Nitro-2-Furyl)-2-Thiazolyl] Formamide (Fanft) Induced Mouse Bladder Tumors

Mickey, Don D.; Mickey, George H.; Murphy, William M.; Niell, Harvey B.; Soloway, Mark S.

doi:10.1016/s0022-5347(17)54305-0

Cited by 34 publications

(16 citation statements)

References 6 publications

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“…The mouse bladder tumor cell line MBT-2 (Mickey et al, 1982) was a generous gift of Dr Guan Wu (Department of Urology, University of Rochester). Cell lines were maintained in DMEM (Life Technologies) supplemented with 10% FBS (Sigma) and penicillin/streptomycin antibiotics (Life Technologies).…”

Section: Tissue Culture and Cell Transfectionsmentioning

confidence: 99%

DBCCR1 mediates death in cultured bladder tumor cells

2004

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Chromosome 9, which is often partially or fully reduced to homozygosity in bladder cancer cells, harbors several tumor suppressor loci including deleted in bladder cancer chromosome region 1 (DBCCR1) at 9q32-33. To study DBCCR1 function, stable cell lines, inducible for DBCCR1 expression by tetracycline, were made, but the DBCCR1 protein was not expressed at detectable levels. To understand the fate of DBCCR1-expressing cells, human bladder tumor cells were transiently transfected with an expression vector containing DBCCR1 fused to enhanced green fluorescent protein (EGFP). Initially, DBCCR1-EGFP-expressing cells demonstrated diffuse cytoplasmic green fluorescence with nuclear exclusion patterns. After time, the intensity level of green fluorescence increased and a granular distribution of protein became visible in the cells. At this point, cells rounded up and detached from the tissue culture dish. Cells transfected with a control vector, containing only EGFP, and partial DBCCR1-EGFP fusion constructs did not demonstrate this behavior. DBCCR1-mediated cell death in cultured tumor cells was independent of caspase-3 activation and did not result in detectable DNA strand breaks by TUNEL staining that are hallmarks of the classical apoptotic pathway.

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Section: Tissue Culture and Cell Transfectionsmentioning

confidence: 99%

DBCCR1 mediates death in cultured bladder tumor cells

2004

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“…The target cell used in this study was N-[4-(5-nitro-2-furyl)-2-thiazolyl] formamide (FANFT)-induced transitional cell carcinoma, MBT-2, from C3H/HeN mice [13].…”

Section: Target Cell For Cytotoxicitymentioning

confidence: 99%

Prostaglandin E2 down-regulates viable Bacille Calmette–Guérin-induced macrophage cytotoxicity against murine bladder cancer cell MBT-2in vitro

Yamada

Kuroda

Matsumoto

et al. 2002

Clinical and Experimental Immunology

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SUMMARY The regulatory effect of prostaglandin (PG) E2 and a cyclooxygenase (COX) inhibitor on Bacille Calmette–Guérin (BCG)‐induced macrophage cytotoxicity in a bladder cancer cell, MBT‐2, was studied in vitro. BCG stimulated thioglycollate‐elicited murine peritoneal exudate cells (PEC) to induce cytotoxic activity and to produce cytokines such as interferon (IFN)‐γ, tumour necrosis factor (TNF)‐α and PGE2. NS398, a specific COX‐2 inhibitor, and indomethacin (IM), a COX‐1 and COX‐2 inhibitor, enhanced viable BCG‐induced cytotoxic activity and IFN‐γ and TNF‐α production of PEC. However, NS398 and IM did not enhance these activities induced by killed BCG. Enhanced cytotoxicity was mediated by increased amounts of IFN‐γ and TNF‐α. Exogenous PGE2 reduced cytotoxic activity and IFN‐γ and TNF‐α production of PEC. These results suggest that PGE2 produced by BCG‐activated macrophages has a negative regulatory effect on the cytotoxic activity of macrophages. Accordingly, a PG synthesis inhibitor may be a useful agent to enhance BCG‐induced antitumour activity of macrophages.

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“…Target cells The target cell line used in this study was N-[4-(5-nitro-2-furyl)-2-thiazolyl]-formamide (FANFT)-induced transitional cell carcinoma MBT-2 from C3H/HeN mice. 17) The cells were maintained continuously by in vitro culture. In vitro culture of peritoneal exudate cells (PEC) PEC were harvested from C3H/HeN mice that had been injected intraperitoneally with 2 ml of thioglycollate (Difco Laboratories Inc.) medium, or 1 ml of OK432 (1KE) 3 days previously.…”

Section: Reagentsmentioning

confidence: 99%