In Vitro Cellular Assays for Oxidative Stress and Biomaterial Response

Mitov, Mihail I.; Patil, Vinod S.; Alstott, Michael; Dziubla, Thomas D.; Butterfield, D. Allan

doi:10.1016/b978-0-12-803269-5.00006-1

Cited by 7 publications

(7 citation statements)

References 139 publications

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“…Another necessary assay involves the detection of oxidative stress as a response to the exposure of cells to nanomaterials. The in vitro assays involve the real-time or static detection of the mechanisms involving oxidative stress, namely the generation of free radicals, metabolites, and degradation products, or the perturbations of redox states [53]. Considering the high consumption of oxygen and polyunsaturated fatty acids, the abundance of redox-active transition metal ions, and the relatively low levels of reduced glutathione, which acts as an antioxidant in the elimination of free radicals, the brain is the organ most susceptible to oxidative stress [54].…”

Section: Toxicity Assessment Of Nanomaterialsmentioning

confidence: 99%

Neurotoxicity of Nanomaterials: An Up-to-Date Overview

et al. 2019

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The field of nanotechnology, through which nanomaterials are designed, characterized, produced, and applied, is rapidly emerging in various fields, including energy, electronics, food and agriculture, environmental science, cosmetics, and medicine. The most common biomedical applications of nanomaterials involve drug delivery, bioimaging, and gene and cancer therapy. Since they possess unique properties which are different than bulk materials, toxic effects and long-term impacts on organisms are not completely known. Therefore, the purpose of this review is to emphasize the main neurotoxic effects induced by nanoparticles, liposomes, dendrimers, carbon nanotubes, and quantum dots, as well as the key neurotoxicology assays to evaluate them.

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Section: Toxicity Assessment Of Nanomaterialsmentioning

confidence: 99%

Neurotoxicity of Nanomaterials: An Up-to-Date Overview

et al. 2019

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“…10-12 Curcumin has also been explored recently for its radical scavenging potential and its capacity to inhibit toxicity of dioxin and dioxin-like molecules such as polychlorinated biphenyl (PCB) and for its potential use in environmental remediation. 13-15 The acrylation approach has similarly been used for other phenolic molecules such as apigenin and quercetin; 16-18 the identification methods discussed in this paper can be extended for these molecules and other phenols, such as myricetin and catechin. 19, 20 …”

mentioning

confidence: 99%

“…These materials improve the stability of curcumin for controlled release and also increase its bioavailability through enhanced solubility. Curcumin acrylation has since been used to make antibacterial nanofibers and chemical sensing materials and as a drug carrier in the form of curcumin microspheres. − Curcumin has also been explored recently for its radical scavenging potential and its capacity to inhibit toxicity of dioxin and dioxin-like molecules such as polychlorinated biphenyl (PCB) and for its potential use in environmental remediation. − The acrylation approach has similarly been used for other phenolic molecules such as apigenin and quercetin; − the identification methods discussed in this paper can be extended for these molecules and other phenols, such as myricetin and catechin. , …”

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confidence: 99%

Curcumin Acrylation for Biological and Environmental Applications

et al. 2017

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Curcumin has recently gained interest for use in drug delivery, chemical sensing, and environmental applications. As a result, the development of synthesis strategies for the incorporation of curcumin into novel materials has become a priority. One such strategy, curcumin acrylation, involves the introduction of acrylate functional groups to the curcumin scaffold, with the potential generation of mono-, di-, and triacrylate curcumin species. The relative populations of these species in the resulting multiacrylate mixture can be controlled by the ratio of curcumin to acryloyl chloride in the initial reaction formulation. Characterization of the acrylation reaction and the resulting curcumin multiacrylate (CMA) product is essential for the effective preparation of new curcumin-containing materials. In this work, a synthesis method for curcumin acrylation is presented and the resulting curcumin multiacrylate product is characterized via various techniques, i.e. HPLC, LCMS, and NMR, as a basis to establish the relationship between synthesis conditions and the extent of acrylation that is achieved.

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“…Importantly, a specific antioxidant molecule should be selected on the basis of the level of stimulation on the cells of the specific tissue target, such as induction of proliferation or differentiation. After the selection of the antioxidant, different endpoints for toxicology assessment can be indicated by in vitro testing [ 101 ], in general, cytotoxicity, cytokine release, and oxidative stress [ 102 ], as well as embryotoxicity and epithelial barrier integrity, according to the tissue origin. Finally, assessing in vitro and in vivo biocompatibility is an essential requirement before the designed biomaterials with gained antioxidant potential would be applied in vivo, in new different tissue environments.…”

Section: New Opportunities For Natural Antioxidant Combination Witmentioning

confidence: 99%

Repositioning Natural Antioxidants for Therapeutic Applications in Tissue Engineering

Marrazzo

O’Leary

2020

Bioengineering

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Although a large panel of natural antioxidants demonstrate a protective effect in preventing cellular oxidative stress, their low bioavailability limits therapeutic activity at the targeted injury site. The importance to deliver drug or cells into oxidative microenvironments can be realized with the development of biocompatible redox-modulating materials. The incorporation of antioxidant compounds within implanted biomaterials should be able to retain the antioxidant activity, while also allowing graft survival and tissue recovery. This review summarizes the recent literature reporting the combined role of natural antioxidants with biomaterials. Our review highlights how such functionalization is a promising strategy in tissue engineering to improve the engraftment and promote tissue healing or regeneration.

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In Vitro Cellular Assays for Oxidative Stress and Biomaterial Response

Cited by 7 publications

References 139 publications

Neurotoxicity of Nanomaterials: An Up-to-Date Overview

Neurotoxicity of Nanomaterials: An Up-to-Date Overview

Curcumin Acrylation for Biological and Environmental Applications

Repositioning Natural Antioxidants for Therapeutic Applications in Tissue Engineering

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