Repositioning Natural Antioxidants for Therapeutic Applications in Tissue Engineering

Marrazzo, Pasquale; O’Leary, Cian

doi:10.3390/bioengineering7030104

Cited by 39 publications

(30 citation statements)

References 258 publications

(525 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H 2 O 2 is generated by the interaction of ascorbic acid with iron ions in the culture medium [ 30 ], and the case of excessive H 2 O 2 production, the enzyme catalase is not able to digest all existing H 2 O 2 into water and it ultimately leads to apoptosis and cell death. It is worth mentioning here that the reactive oxygen species (ROS), which are produced as a result of cellular response to oxidative stress, has been associated with graft failure [ 31 ]. Often, long-term exposure to hypoxia can induce formation of ROS [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering

Rashidbenam

Jasman

Tan

et al. 2021

IJMS

View full text Add to dashboard Cite

Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 104 cells/cm2 seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries.

show abstract

Section: Discussionmentioning

confidence: 99%

Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering

Rashidbenam

Jasman

Tan

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Scaffolds play a crucial role in maintaining and controlling cell functions and are the main responsible for the biomechanical properties of the bioartificial tissues [ 3 ]. Therefore, biomaterials used as scaffolds in tissue engineering should display adequate biomechanical properties, along with adequate biocompatibility and other characteristics such as bioresorption or biodegradation, adequate internal morphology and cell-friendly fabrication [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Marine Agarose Biomaterials for Tissue Engineering Applications

Irastorza-Lorenzo

Sánchez-Porras

Ortiz-Arrabal

et al. 2021

IJMS

View full text Add to dashboard Cite

Five agarose types (D1LE, D2LE, LM, MS8 and D5) were evaluated in tissue engineering and compared for the first time using an array of analysis methods. Acellular and cellular constructs were generated from 0.3–3%, and their biomechanical properties, in vivo biocompatibility (as determined by LIVE/DEAD, WST-1 and DNA release, with n = 6 per sample) and in vivo biocompatibility (by hematological and biochemical analyses and histology, with n = 4 animals per agarose type) were analyzed. Results revealed that the biomechanical properties of each hydrogel were related to the agarose concentration (p < 0.001). Regarding the agarose type, the highest (p < 0.001) Young modulus, stress at fracture and break load were D1LE, D2LE and D5, whereas the strain at fracture was higher in D5 and MS8 at 3% (p < 0.05). All agaroses showed high biocompatibility on human skin cells, especially in indirect contact, with a correlation with agarose concentration (p = 0.0074 for LIVE/DEAD and p = 0.0014 for WST-1) and type, although cell function tended to decrease in direct contact with highly concentrated agaroses. All agaroses were safe in vivo, with no systemic effects as determined by hematological and biochemical analysis and histology of major organs. Locally, implants were partially encapsulated and a pro-regenerative response with abundant M2-type macrophages was found. In summary, we may state that all these agarose types can be safely used in tissue engineering and that the biomechanical properties and biocompatibility were strongly associated to the agarose concentration in the hydrogel and partially associated to the agarose type. These results open the door to the generation of specific agarose-based hydrogels for definite clinical applications such as the human skin, cornea or oral mucosa.

show abstract

“…The variable quality and quantity of phenolics are significant contributors to a wide range of biological effects and antioxidant capacity in honey of different origins [ 77 ]. This antioxidant potential may be useful for developing tunable systems that sense and regulate the oxidative stress level after the implantation of tissue-engineered constructs [ 78 ]. However, honey variation is great and depends on many factors, such as the seasonal collection time and botanical source.…”

Section: Resultsmentioning

confidence: 99%

The Potential of Honeybee Products for Biomaterial Applications

Rossi

Marrazzo

2021

Biomimetics

Self Cite

View full text Add to dashboard Cite

The development of biomaterials required continuous improvements in their properties for new tissue engineering applications. Implants based on biocompatible materials and biomaterial-based dressings are susceptible to infection threat; moreover, target tissues can suffer injuring inflammation. The inclusion of nature-derived bioactive compounds usually offers a suitable strategy to expand or increase the functional properties of biomaterial scaffolds and can even promote tissue healing. Honey is traditionally known for its healing property and is a mixture of phytochemicals that have a proven reputation as antimicrobial, anti-inflammatory, and antioxidant agents. This review discusses on the potential of honey and other honeybee products for biomaterial improvements. Our study illustrates the available and most recent literature reporting the use of these natural products combined with different polymeric scaffolds, to provide original insights in wound healing and other tissue regenerative approaches.

show abstract

Repositioning Natural Antioxidants for Therapeutic Applications in Tissue Engineering

Cited by 39 publications

References 258 publications

Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering

Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering

Evaluation of Marine Agarose Biomaterials for Tissue Engineering Applications

The Potential of Honeybee Products for Biomaterial Applications

Contact Info

Product

Resources

About