In-vitro Callus Induction of Durian (Durio zibethinus Murr.) Leaves Using Kinetin and 2,4-D (Dichlorophenoxyacetic acid)

Abstract

Durian (Durio zibethinus Murr.) is a tropical fruit grown in Southeast Asia and it has high nutritional and economic values and highly appreciated by consumers in Indonesia. Unfortunately, there are several obstacles in planting durian in Indonesia, specifically in Aceh. One of the abstacles is the scarcity of superior durian plants. Generally, plant conservations done conventionally through vegetative propagation. However, this technique has disadvantage such as harming the initial plants. Therefore, plant culture tissue has been used as a modern technique to develop durian plants. The objective of this research was to evaluate the effect of kinetin and 2,4 D on development of durian leaf explants through in-vitro bioassay. The research was conducted at Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University from January to April 2019 using Completely Randomized Design (CRD) Factorial with 2 factors observed. The first factor was Kinetin (K): 0.0, 0.1 and 0.5 ppm. The second factor was auxin 2,4 D: 0.0, 0.5 and 1.0 ppm. The results showed that the in-vitro application of kinetin affected the induction of leaf callus. The application of 0.5 ppm of kinetin gave the best result compared to others. The in-vitro application of 2,4 D 0.5 ppm also possessed the best result compared to others. There was no interaction beween in-vitro application of kinetin and 2,4 D on the growth of durian leaf callus at all parameters observed.

In Vitro Regeneration Potential of Thin Cell Layer Explants of Lentisk (Pistacia lentiscus var. Chia) Plant

“…The most commonly used growth regulators in plant tissue culture technology are auxins and cytokines [41].The rate of use of auxin and cytokinin may vary according to what kind of morphogenetic improvement we want. The combined use of 2,4-D (auxin) and KIN (cytokinin) growth regulators in the nutrient medium often stimulates callus formation or somatic embryo formation [42]. In callus culture initiated from different plant parts in Moringa plant, the best callus development was achieved with 5 mg/L 2,4-D and 0.1 mg/L in leaf-based tissues [43].…”

Pertumbuhan dan Hasil Tanaman Selada (Lactuca Sativa L.) Akibat Perlakuan Pupuk Kandang Jangkrik dan Pupuk Kandang Ayam

“…Penelitian berbagai jenis tanaman hortikultura telah mulai dilakukan tim penelitian Fakultas Pertanian Universitas Malikussaleh. Jenis tanaman buah yang sudah diteliti adalah tanaman durian (Handayani & Ismadi 2017;Handayani et al 2019;Liwanza et al, 2019), manggis , alpukat (Ismadi et al 2018); (Ismadi et al 2019), nanas (Liwanza et al, 2022). Jenis tanaman sayuran yang sudah diteliti adalah bawang daun (Paiman et al 2019), dan pakcoy (Usnawiyah et al, 2022), Tujuan penelitian untuk mengetahui adanya pengaruh pupuk kandang jangkrik dan kandang ayam terhadap pertumbuhan dan hasil tanaman selada.…”

Induksi kalus daun binahong merah (Basella rubra L.) dengan pe,berian 2,4-D dan kinetin

“…Penggunaan auksin bersama dengan sitokinin memungkinkan kita untuk menentukan jenis morfogenesis yang diinginkan. 2,4-D yang merupakan golongan auksin dan kinetin yang merupakan golongan sitokinin merupakan zat pengatur tumbuh sintetik yang umumnya digunakan dalam penelitian kultur jaringan (Handayani et al, 2019).…”