In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

Menin, Barbara; Moglia, Andrea; Comino, Cinzia; Lanteri, Sergio; Herpen, T.W.J.M. van; Beekwilder, Jules

doi:10.17660/actahortic.2012.961.34

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…The analyses of the transgenic lines proved that the CaMV35S promoter is suitable for overexpression strategies also in cardoon cells, as already shown for other Asteraceae such as artichoke [ 62 ], chicory ( Cichorium intybus var. sativum L., [ 63 ]), and gerbera ( Gerbera jamesonii , [ 64 ]).…”

Section: Discussionsupporting

confidence: 53%

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Paolo

Locatelli

Cominelli

et al. 2021

IJMS

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Cultivated cardoon (Cynara cardunculus var. altilis L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for Agrobacterium tumefaciens-mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the Arabidopsis thaliana MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of AtMYB4 overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.

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Section: Discussionsupporting

confidence: 53%

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Paolo

Locatelli

Cominelli

et al. 2021

IJMS

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“…While a mature regeneration/transformation technology is available for both above-mentioned species, de novo shoot regeneration and cell transformation have been observed only sporadically and with very low efficiency in another important vegetable of this family, i.e., globe artichoke ( Cynara cardunculus var. scolymus ; Menin et al, 2012 ).…”

Section: Regeneration and Transformationmentioning

confidence: 99%

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

2017

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In the frame of modern agriculture facing the predicted increase of population and general environmental changes, the securement of high quality food remains a major challenge to deal with. Vegetable crops include a large number of species, characterized by multiple geographical origins, large genetic variability and diverse reproductive features. Due to their nutritional value, they have an important place in human diet. In recent years, many crop genomes have been sequenced permitting the identification of genes and superior alleles associated with desirable traits. Furthermore, innovative biotechnological approaches allow to take a step forward towards the development of new improved cultivars harboring precise genome modifications. Sequence-based knowledge coupled with advanced biotechnologies is supporting the widespread application of new plant breeding techniques to enhance the success in modification and transfer of useful alleles into target varieties. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system, zinc-finger nucleases, and transcription activator-like effector nucleases represent the main methods available for plant genome engineering through targeted modifications. Such technologies, however, require efficient transformation protocols as well as extensive genomic resources and accurate knowledge before they can be efficiently exploited in practical breeding programs. In this review, we revise the state of the art in relation to availability of such scientific and technological resources in various groups of vegetables, describe genome editing results obtained so far and discuss the implications for future applications.

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Globe Artichoke Tissue Culture and Its Biotechnological Application

Comino

Moglia

Repetto

et al. 2019

Compendium of Plant Genomes

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In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

Cited by 5 publications

References 16 publications

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

Globe Artichoke Tissue Culture and Its Biotechnological Application

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