In vitro Biological Activity of Germacranolide sesquiterpene lactones

Abstract

Bioactivity-directed isolation has led to the isolation of (-)-ent-costunolide (1) as the major active compound from Hepatostolonophora paucistipula. This compound (1) was determined by spectroscopic data interpretation. This sesquiterpene lactone (1) inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (4 mm inhibition zone at 15 µg/ disc), cytotoxic activity to murine leukaemia cell lines ATCC CCL 46 P 388D1 (IC 5 0 687 ng/ml, at 0.075 µg/disk), BSC monkey kidney cell lines (100% of well at 15 µg/disk) and antiviral activity to Herpes simplex virus (0.25 mg/ml, 100% of well at 7.5 µg/disk) and Polio virus (0.125 mg/ml, 100% of well at 3.75 µg/disk). These results suggest that (-)-ent-costunolide (1) has potential antimicrobial and cytotoxic agents.

Antifungal Effect of Brachyglottis repanda Ethanol Extract

“…However, the ethanol extract of B. monroi (25% activity, 5 mg/ml at 75 μg/disk) indicated stronger antiviral activity against Herpes simplex Type I virus ATCC VR 733 than that of B. repanda (Kwag et al , 2004). This ethanol extract did not show any antimicrobial activity against microorganisms (Table 2) (Yook et al , 2007; Kim et al , 2009).…”

“…However, the other fractions were inactive (Fig. 2 and Table 3) (Yook et al , 2007; Kim et al , 2009). The separation of the main components from the bioactive fraction 64-3 of B. repanda extract needs be studied further and the results will be discussed elsewhere.…”