In vitro biofilm formation by multidrug resistant clinical isolates of Pseudomonas aeruginosa

Sanjee, Sohana Al; Hassan, Mahamudul; Manchur, MA

doi:10.3329/ajmbr.v4i1.36828

Cited by 2 publications

(1 citation statement)

References 32 publications

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“…[8][9][10] Biofilm forming P. aeruginosa and S. aureus and their resistance to antibiotics are reported earlier. 11,12 Additionally, multidrug resistant (MDR) Escherichia coli was observed in non-clinical sources. 13 The genetic flexibility and adaptability of E. coli allow them to develop AR mechanisms based on the changes in their environments.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Profiling and Molecular Phylogeny of Biofilm Forming Bacteria From Clinical and Non-clinical Environment in Southern Part of Bangladesh

Nain

Islam

Karim

2019

Int J Enteric Pathog

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Background: Biofilm is a surface adhered extracellular polymer matrix produced by bacteria. The establishment of biofilms is considered as an important pathogenic trait in many chronic infections and antibiotic resistance. Objective: The present study was intended to evaluate biofilm forming potency and antibiotic resistance (AR) pattern in clinical and non-clinical bacterial isolates, and their phylogenetic characterization. Materials and Methods: A total of 82 bacterial isolates were obtained from clinical settings and animal farms from southern (Kushtia-Jhenaidah) region of Bangladesh. Biofilm forming potentials and AR profile were evaluated by standard biofilm assay and Kirby-Bauer disk diffusion method, respectively. Further, antibiotic exposure was assessed by multiple antibiotic resistance (MAR) value indexing. Furthermore, statistical methods were applied to estimate the relationship between AR and biofilm formation. Finally, selected isolates were characterized by morphological and biochemical tests, as well as 16S rRNA gene sequencing. Results: Clinical isolates showed higher biofilm formation (OD595=1.17±0.03) than non-clinical isolates (OD595=0.68±0.03). Among all, Pseudomonas isolates produced the highest amount of biofilms (OD595=2.08±0.02). The AR profiles fell within 46.67-86.67% and MAR index ranged from 0.47 to 0.87. Moreover, a significant positive correlation (P<0.05) was found between biofilm formation and AR. Eventually, heavy biofilm producers with ≥60% resistance profile were characterized and identified as Escherichia coli, Cronobacter sakazakii, Pseudomonas aeruginosa, Staphylococcus sciuri, and Staphylococcus aureus. Conclusion: In general, biofilm formation and MAR were highly correlated regardless of the source, type, and environment of the isolates. Therefore, a rigorous evaluation of both biofilm formation and AR is demanded to minimize AR and associated problems.

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Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Profiling and Molecular Phylogeny of Biofilm Forming Bacteria From Clinical and Non-clinical Environment in Southern Part of Bangladesh

Nain

Islam

Karim

2019

Int J Enteric Pathog

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The enhanced antibacterial and antibiofilm properties of titanium dioxide nanoparticles biosynthesized by multidrug-resistant Pseudomonas aeruginosa

Haji,

Ganjo,

Faraj

et al. 2024

BMC Microbiol

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The emergence of Multidrug-resistant (MDR) bacteria are becoming a major worldwide health concern, encouraging the development effective alternatives to conventional antibiotics. The study identified P. aeruginosa and assessed its antimicrobial sensitivity using the Vitek-2 system. Carbapenem-resistant genes were detected through Polymerase chain reaction (PCR). MDR- P. aeruginosa isolates were used to biosynthesize titanium dioxide nanoparticles (TiO2NPs) and characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM). A study involving 78 P. aeruginosa isolates revealed that 85.8% were MDR, with meropenem and amikacin showing effectiveness against 70% of the isolates. The most prevalent carbapenemase gene was blaOXA-48, present in 83% of the isolates. Majority of the isolates formed biofilms, and biosynthesized TiO2NPs were able to reduce biofilm formation by 94%. TiO2NPs exhibited potent antibacterial action against MDR-Gram-negative bacilli pathogens and showed synergistic activity with antibiotics, particularly piperacillin, with a significant fold increase in areas (283%). A new local strain of P. aeruginosa, identified as ON678251 in the World GenBank, was found capable of producing TiO2NPs. Our findings demonstrate the potential of biosynthesized TiO2NPs to manage antibiotic resistance and regulate the formation of biofilms. This presents a promising direction for the creation of novel antimicrobial agents or substitutes for use in clinical settings, particularly in the management of isolates capable of resisting multiple drugs.

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In vitro biofilm formation by multidrug resistant clinical isolates of Pseudomonas aeruginosa

Cited by 2 publications

References 32 publications

Antibiotic Resistance Profiling and Molecular Phylogeny of Biofilm Forming Bacteria From Clinical and Non-clinical Environment in Southern Part of Bangladesh

Antibiotic Resistance Profiling and Molecular Phylogeny of Biofilm Forming Bacteria From Clinical and Non-clinical Environment in Southern Part of Bangladesh

The enhanced antibacterial and antibiofilm properties of titanium dioxide nanoparticles biosynthesized by multidrug-resistant Pseudomonas aeruginosa

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