In vitro biocompatibility evaluation of silk-fibroin/polyurethane membrane with cultivation of HUVECs

Zhou, Mingyang; Wang, Wei-ci; Liao, Yonggui; Liu, Wenqi; Yu, Miao; Ouyang, Chenxi

doi:10.1007/s11706-014-0230-3

Cited by 11 publications

(4 citation statements)

References 7 publications

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“…A CCK-8 kit was used to evaluate the proliferation and toxicity of cells, as described previously. 40 In brief, the various microfiber scaffolds (20 mm × 30 mm) were first sterilized and fixed on a 24-well plate. One milliliter of DMEM solution with 10% FBS and 1% PS (100 U/ml penicillin and 100 μg/ml streptomycin) was added to each well, and the HUVEC density was 2.50 × 10 4 cells/ml.…”

Section: In Vitro Biocompatibility Evaluationmentioning

confidence: 99%

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Liu

Chen

et al. 2020

Journal of Bioactive and Compatible Polymers

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Long-distance (⩾10 mm) arterial vascular defect injury was a massive challenge affecting human health. Compared with autologous transplantation, tissue-engineered scaffolds such as biocompatible silk fibroin (SF) scaffolds have been developed because they exhibit equivalent functional repair effects without adverse reactions. However, its mechanical strength and structural stability needed to be further improved to match the longer repair cycle of blood vessels while maintaining the original biological safety. Hence, we designed and prepared SF and hydrophobic polycaprolactone (PCL) composite microfibers by an improving electrospinning method. It was found that when the weight ratio of PCL to SF was 1: 1, a microfiber scaffold with high strength (6.16 N) and minimum degradability can be obtained. More importantly, compared with natural silk fibroin, the novel composite microfiber scaffolds can slightly inhibit cell infiltration and inflammation through co-culture with HUVECs in vitro and rabbit back transplantation in vivo. Furthermore, the fabricated scaffolds also demonstrated excellent structural stability in vivo because of the well-organized PCL doping in the structure. All these results indicated that the novel PCL/SF composite microfiber scaffolds were promising candidates for vascular tissue engineering applications.

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Section: In Vitro Biocompatibility Evaluationmentioning

confidence: 99%

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Liu

Chen

et al. 2020

Journal of Bioactive and Compatible Polymers

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“…This device, intended for by-pass grafting or as vascular access, has been implanted as total cavopulmonary connection in paediatric patients in a pioneering clinical trial [19,20]. Another approach consists in blending different materials, natural and/or synthetic, in order to maximize scaffold's properties and characteristics [21][22][23][24][25][26], and, in particular, the combination of silk fibroin (SF), a biodegradable and highly biocompatible protein, and polyurethanes (PUs), was proved to be promising hybrid solution in TE [27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Hybrid fibroin/polyurethane small-diameter vascular grafts: from fabrication to in vivo preliminary assessment

et al. 2022

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To address the need of alternatives to autologous vessels for small-calibre vascular applications (e.g. cardiac surgery), a hybrid semi-degradable material composed of silk fibroin and polyurethane (Silkothane®) was herein used to fabricate very small-calibre grafts (innner diameter = 1.5 mm) via electrospinning. Hybrid grafts were in vitro characterized in terms of morphology and mechanical behaviour, and compared to similar grafts of pure silk fibroin. Similarly, two native vessels from a rodent model (abdominal aorta and vena cava) were harvested and characterized. Preliminary implants were performed on Lewis rats to confirm the suitability of Silkothane® grafts for small-calibre applications, specifically as aortic insertion and femoral shunt. The manufacturing process generated pliable grafts consisting of a randomized fibrous mesh and exhibiting similar geometrical features to rat aortas. Both Silkothane® and pure silk fibroin grafts showed radial compliances in the range from 1.37 ± 0.86 to 1.88 ± 1.01 % 10-2 mmHg-1, lower than that of native vessels. The Silkothane® small-calibre devices were also implanted in rats demonstrating to be adequate for vascular applications; all the treated rats survived the surgery for 3 months after implantation, and 16 rats out of 17 (94%) still showed blood flow inside the graft at sacrifice. The obtained results lay the basis for a deeper investigation of the interaction between the Silktohane® graft and the implant site, which may deal with further analysis on the potentialities in terms of degradability and tissue formation, on longer time-points.

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“…Human umbilical vein endothelial cells (HUVECs) grown on the surface of NSFP-modified BPU membranes showed good spreading and adhesion. 16 However, the in vitro and in vivo biocompatibilities of BPU and NSFP composites and their relationship to the physical properties of these hybrid membranes are unknown.…”

Section: Introductionmentioning

confidence: 99%

The effect of native silk fibroin powder on the physical properties and biocompatibility of biomedical polyurethane membrane

Zhuang

Zhang

Feng

et al. 2017

Proc Inst Mech Eng H

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Naturally derived fibers such as silk fibroin can potentially enhance the biocompatibility of currently used biomaterials. This study investigated the physical properties of native silk fibroin powder and its effect on the biocompatibility of biomedical polyurethane. Native silk fibroin powder with an average diameter of 3 µm was prepared on a purpose-built machine. A simple method of phase inversion was used to produce biomedical polyurethane/native silk fibroin powder hybrid membranes at different blend ratios by immersing a biomedical polyurethane/native silk fibroin powder solution in deionized water at room temperature. The physical properties of the membranes including morphology, hydrophilicity, roughness, porosity, and compressive modulus were characterized, and in vitro biocompatibility was evaluated by seeding the human umbilical vein endothelial cells on the top surface. Native silk fibroin powder had a concentration-dependent effect on the number and morphology of human umbilical vein endothelial cells growing on the membranes; cell number increased as native silk fibroin powder content in the biomedical polyurethane/native silk fibroin powder hybrid membrane was increased from 0% to 50%, and cell morphology changed from spindle-shaped to cobblestone-like as the native silk fibroin powder content was increased from 0% to 70%. The latter change was related to the physical characteristics of the membrane, including hydrophilicity, roughness, and mechanical properties. The in vivo biocompatibility of the native silk fibroin powder-modified biomedical polyurethane membrane was evaluated in a rat model; the histological analysis revealed no systemic toxicity. These results indicate that the biomedical polyurethane/native silk fibroin powder hybrid membrane has superior in vitro and in vivo biocompatibility relative to 100% biomedical polyurethane membranes and thus has potential applications in the fabrication of small-diameter vascular grafts and in tissue engineering.

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In vitro biocompatibility evaluation of silk-fibroin/polyurethane membrane with cultivation of HUVECs

Cited by 11 publications

References 7 publications

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Development of an electrospun polycaprolactone/silk scaffold for potential vascular tissue engineering applications

Hybrid fibroin/polyurethane small-diameter vascular grafts: from fabrication to in vivo preliminary assessment

The effect of native silk fibroin powder on the physical properties and biocompatibility of biomedical polyurethane membrane

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