In vitro biocompatibility, antibacterial activity, and release behavior of halloysite nanotubes loaded with diclofenac sodium salt incorporated in electrospun soy protein isolate/hydroxyethyl cellulose nanofibers

Ullah, Azeem; Sarwar, Muhammad Nauman; Wang, Feifei; Kharaghani, Davood; Sun, Lei; Zhu, Chunhong; Yoshiko, Yuji; Mayakrishnan, Gopiraman; Lee, Jung-soon; Kim, Ick Soo

doi:10.1016/j.crbiot.2022.09.008

Cited by 14 publications

(5 citation statements)

References 51 publications

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“…To check for toxicity and cell viability, we conducted an LDH and WST-1 cytotoxicity analysis for the NIH3T3 cell line, while it was incubated with composite films. The LDH results ( Figure 13 a) revealed that the LDH released from the damaged cells was less than 15% in all cases and fell within acceptable levels of toxicity [ 11 ]. The one-way ANOVA showed that the addition of pumpkin powder significantly increased the LDH toxicity, with an R 2 of 0.9236 and a p -value of 0.002.…”

Section: Resultsmentioning

confidence: 99%

“…The addition of niaouli oil resulted in a slight decrease in cell viability, which can be seen from the one-way ANOVA values of an R 2 of 0.9309 and a p -value of 0.00). However, the overall viability still remained above 80%, which gave the prepared films a nontoxic status according to the ISO-10993-5 standard [ 11 , 74 , 75 , 76 ].…”

Section: Resultsmentioning

confidence: 99%

“…Despite PVA’s unique properties, some of these properties must be modified or enhanced to extend their potential usage [ 9 , 10 ]. Therefore, the preparation of a composite film by adding functional biomaterials and obtaining a blend film could be a potential strategy to overcome the drawback of PVA being a synthetic polymer [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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PVA/Inulin-Based Sustainable Films Reinforced with Pickering Emulsion of Niaouli Essential Oil for Potential Wound Healing Applications

et al. 2023

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In this study, sustainable water-based films were produced via the solvent-casting method. Petroleum-free-based polyvinyl alcohol (PVA) and carbohydrate-based inulin (INL) were used as matrices. Vegetable-waste pumpkin powder was used in the study because of its sustainability and antibacterial properties. Pickering emulsions were prepared using β-cyclodextrin. The influence of the different ratios of the β-cyclodextrin/niaouli essential oil (β-CD/NEO) inclusion complex (such as 1:1, 1:3, and 1:5) on the morphological (SEM), thermal (TGA), physical (FT-IR), wettability (contact angle), and mechanical (tensile test) characteristics of PVA/inulin films were investigated. Moreover, the antibacterial activities against the Gram (−) (Escherichia coli and Pseudomonas aeruginosa) and Gram (+) (Staphylococcus aureus) bacteria of the obtained films were studied. From the morphological analysis, good emulsion stability and porosity were obtained in the Pickering films with the highest oil content, while instability was observed in the Pickering films with the lowest concentration of oil content. Thermal and spectroscopic analysis indicated there was no significant difference between the Pickering emulsion films and neat films. With the addition of Pickering emulsions, the tensile stress values decreased from 7.3 ± 1.9 MPa to 3.3 ± 0.2. According to the antibacterial efficiency results, films containing pumpkin powder and Pickering emulsion films containing both pumpkin powder and a ratio of 1:1 (β-CD/NEO) did not have an antibacterial effect, while Pickering emulsion films with a ratio of (β-CD/NEO) 1:3 and 1:5 showed an antibacterial effect against Escherichia coli, with a zone diameter of 12 cm and 17 cm, respectively. Among the samples, the films with ratio of (β-CD/NEO) 1:5 had the highest antioxidant capacity, as assessed by DPPH radical scavenging at 12 h intervals. Further, none of the samples showed any cytotoxic effects the according to LDH and WST-1 cytotoxicity analysis for the NIH3T3 cell line. Ultimately, it is expected that these films are completely bio-based and may be potential candidates for use in wound healing applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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PVA/Inulin-Based Sustainable Films Reinforced with Pickering Emulsion of Niaouli Essential Oil for Potential Wound Healing Applications

et al. 2023

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“…The presence of mineral nanotubes ensured sustained drug release from the mats for over two weeks. The composite showed antibacterial properties as well as cell biocompatibility in vitro [ 73 ].…”

Section: Recent Trends In Biomedical Application Of Electrospun Plant...mentioning

confidence: 99%

Agro-Industrial Plant Proteins in Electrospun Materials for Biomedical Application

et al. 2023

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Plant proteins are receiving a lot of attention due to their abundance in nature, customizable properties, biodegradability, biocompatibility, and bioactivity. As a result of global sustainability concerns, the availability of novel plant protein sources is rapidly growing, while the extensively studied ones are derived from byproducts of major agro-industrial crops. Owing to their beneficial properties, a significant effort is being made to investigate plant proteins’ application in biomedicine, such as making fibrous materials for wound healing, controlled drug release, and tissue regeneration. Electrospinning technology is a versatile platform for creating nanofibrous materials fabricated from biopolymers that can be modified and functionalized for various purposes. This review focuses on recent advancements and promising directions for further research of an electrospun plant protein-based system. The article highlights examples of zein, soy, and wheat proteins to illustrate their electrospinning feasibility and biomedical potential. Similar assessments with proteins from less-represented plant sources, such as canola, pea, taro, and amaranth, are also described.

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“…These fibers typically range in diameter from tens of nanometers to a few micrometers [11]. In addition to being involved in drug delivery systems [12], nanofibers have been employed for the regeneration of various tissues, including wound dressing [13], bone [14], cardiovascular [15], and blood vessels [16]. For 3D scaffold design, both the material and fabrication method should be carefully considered, as the composition and structure of the ECM regulate the scaffold network's structure and biomechanical properties.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Polymeric Nanofibers Used for 3D-Printed Scaffolds on Cellular Activity in Tissue Engineering: A Review

Kharaghani

Kaffashsaei

Haider

et al. 2023

IJMS

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Promising scaffolds for developing advanced tissue engineering architectures have emerged in recent years through the use of nanofibers and 3D printing technologies. Despite this, structural integrity and cell proliferation are highlighted as fundamental challenges for design scaffolds and future prospects. As a biomimetic scaffold, the nanofiber-reinforced hydrogels demonstrated a better compressive modulus and cell growth. Our review focuses on recent promising advances in the development of 3D-printed hydrogels containing polymeric nanofibers that can improve cell-material interaction in biomedical applications. Moreover, an effort has been made to induce studies with diverse types of scaffolds for various cells. Additionally, we discuss the challenges and future prospects of 3D-bioprinted reinforced hydrogels with nanofibers in the medical field, as well as high-performance bioinks.

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In vitro biocompatibility, antibacterial activity, and release behavior of halloysite nanotubes loaded with diclofenac sodium salt incorporated in electrospun soy protein isolate/hydroxyethyl cellulose nanofibers

Cited by 14 publications

References 51 publications

PVA/Inulin-Based Sustainable Films Reinforced with Pickering Emulsion of Niaouli Essential Oil for Potential Wound Healing Applications

PVA/Inulin-Based Sustainable Films Reinforced with Pickering Emulsion of Niaouli Essential Oil for Potential Wound Healing Applications

Agro-Industrial Plant Proteins in Electrospun Materials for Biomedical Application

The Effect of Polymeric Nanofibers Used for 3D-Printed Scaffolds on Cellular Activity in Tissue Engineering: A Review

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