In Vitro Bioavailability Study of an Antiviral Compound Enisamium Iodide

Haltner-Ukomadu, Eleonore; Gureyeva, S. N.; Burmaka, Oleksii; Goy, A. M.; Mueller, Lutz; Kostyuk, Grygorii; Margitich, Victor

doi:10.3390/scipharm86010003

Cited by 6 publications

(6 citation statements)

References 23 publications

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“…The culture medium was replaced every 2-3 days. After 21 days of culture, the cells were exposed for 24 h to sodium dodecyl sulfate (SDS) solution 0.5 mM, a well-known substance impacting the integrity of Caco-2 cells, used as positive control for cytotoxicity evaluation [32,33]. After SDS exposure the media was collected and used for analysis on the multiparametric (bio)sensing platform.…”

Section: Cell Culturementioning

confidence: 99%

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Chmayssem

Verplanck

Tanase

et al. 2021

Talanta

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There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the developed platform allows a multiparametric analysis instead of single-analyte analysis. Here we provide an overview of the sensors-based analysis of four main factors that can indicate a possible cell deterioration problem during cell-culture: pH, hydrogen peroxide, nitric oxide/nitrite and lactate. Herein, we are proposing a sensors platform based on thick-film coupled to microfluidic technology that can be integrated into any microfluidic system using Luer-lock connectors. This platform allows obtaining an accurate analysis of the secreting stress metabolites during cell/tissues culture.

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Section: Cell Culturementioning

confidence: 99%

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Chmayssem

Verplanck

Tanase

et al. 2021

Talanta

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“…При этом в клетках MDCK, традиционно используемой как пермиссивная линия для роста вирусов гриппа и поиска этиотропных противогриппозных препаратов, энисамия йодид активности не имеет [12]. Это обусловлено различной проницаемостью клеток разных линий для молекул препарата [20] и проявляется в различной его противовирусной активности. Известно, что применение энисамия йодида ограничивает вирусную репродукцию на ранних стадиях вирусного цикла, однако выяснение мишени и механизма действия препарата требует дальнейших исследований.…”

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Anti-viral activity of enisamium iodide against viruses of influenza and ARVI’s on different cell lines

Zarubaev

Slita

Синегубова

et al. 2020

Terapevticheskii arkhiv

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Influenza and ARVI represent the most numerous and dangerous group of causative agents of respiratory infections human. Aim. Characterization of the antiviral properties of enisamium iodide against human respiratory viruses in in vitro experiments. Materials and methods. In the course of experiments, the cytotoxic properties of enisamium iodide were studied against the cell lines Vero, MA-104, A549, L-41 and HEp-2. The antiviral activity of enisamium iodide was studied using virus yield reduction assay against influenza viruses, parainfluenza virus, respiratory syncytial virus, Coxsackie B3 and Coxsackie B4 viruses, as well as adenoviruses types 5 and 6. Results. The most sensitive to the action of enisamium iodide was the human parainfluenza virus, whose activity decreased by 2.3 orders of magnitude under the action of the drug in A549 cells. Of the cell cultures used, enisamium iodide exhibited the maximum antiviral effect in human lung carcinoma cells A549, where, in its presence, the level of reproduction of adenoviruses of types 5 and 6, Coxsackie viruses B3 and B4, and human parainfluenza virus decreased by an order of magnitude or more. The antiviral activity of enisamium iodide was least manifested in Vero cells. Conclusion. According to the results of in vitro experiments, enisamium iodide can be considered as an antiviral drug with a wide spectrum of activity against human respiratory viruses.

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“…The lysosomotropic mechanism may play an important role, since amixin blocks the virus entry into cells. Cationic amphiphilic agents have recently been proposed as a useful starting point for broad-spectrum antivirals [7]. Amixin was previously identified in vitro as a candidate drug against SARS-CoV-2 [8].…”

Section: Fig 2 Structural Formula Of Amixinementioning

confidence: 99%

Development of a method for the detection of amixin and amizon by HPLC on SunFire C18 column

Belikova¹,

Материенко²,

Sidorenko³

et al. 2022

chemija

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A method for the determination of amizone and amixin in the same sample and in their mixture with antibiotics (ceftriaxone, tetracycline, ampicillin and levofloxacin) was developed using reversed-phase highperformance liquid chromatography equipped with a photodiode array detector. A SunFire C18 column, a mobile phase consisting of sodium perchlorate buffer (pH = 2.5) and acetonitrile (75:25), at a flow rate of 0.8 ml/min, was used. The analytes were identified at 205 and 265 nm. The specificity, linearity, precision parameters, LOD and LOQ were evaluated during the validation of the methodology, and the correlation coefficients of amizone and amixin were 0.9992 and 0.9998, respectively. This method can also be used for the determination of amizone and amixin and their presence in a mixture with antibiotics in the environment.

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In Vitro Bioavailability Study of an Antiviral Compound Enisamium Iodide

Cited by 6 publications

References 23 publications

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites

Anti-viral activity of enisamium iodide against viruses of influenza and ARVI’s on different cell lines

Development of a method for the detection of amixin and amizon by HPLC on SunFire C18 column

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