In vitro bioactivity of bioresorbable porous polymeric scaffolds incorporating hydroxyapatite microspheres

Li, L. H.; Kommareddy, Krishna P.; Pilz, Christine; Zhou, Changren; Fratzl, Peter; Manjubala, I.

doi:10.1016/j.actbio.2009.03.028

Cited by 73 publications

(52 citation statements)

References 21 publications

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“…Their investigation indicated that the composite microsphere-sintered scaffold is a promising scaffold for bone repair. It is reported that PCL mixed with other biodegradable inorganic materials such as hydroxyapatite (HA) [6][7][8][9][10] and tricalcium phosphate (TCP) [11][12][13] could improve some properties so that the composites may offer attractive potential for bone tissue replacement and tissue regeneration. Besides HA and TCP, CS as a biodegradable inorganic material is widely used in bone surgery as well.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of polycaprolactone/calcium sulfate whisker composites

Liu¹,

Reni²,

Wei³

et al. 2011

Express Polym. Lett.

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Abstract. Polycaprolactone (PCL)/calcium sulfate (CS) whisker composites have been fabricated by melt blending and coprecipitation methods respectively. Scanning electron microscope (SEM) was used to observe the microstructure of the composites. The crystallization and thermal properties were characterized by polarized optical microscope (POM), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). For composites prepared by melt blending method, experiment results show that average length of the whiskers is shortened. The crystallization perfection of PCL in composites is improved by adding whiskers. The flexural strength increases whereas the impact strength decreases. For composites prepared by coprecipitation method, whisker addition worsens the crystallization perfection of PCL. An improvement of 21% in flexural strength and 22% in impact strength has been achieved for the composite with 15 wt% of whiskers.

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Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of polycaprolactone/calcium sulfate whisker composites

Liu¹,

Reni²,

Wei³

et al. 2011

Express Polym. Lett.

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“…Because of the importance of nano-sized structures of the extracellular matrix (ECM) in the regulation of many cell functions, scaffolds with nanoscale morphology have been vigorously studied [9,10] . In this regard, developing nanofibrous scaffolds that mimic the architecture of tissue at the nanoscale can potentially meet this challenge [12] . Irrespective of the method of synthesis, nanofibers have been used as scaffolds for musculoskeletal tissue engineering (including bone, cartilage, ligament, and skeletal Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering www.chinaphar.com Bakhshandeh B et al Acta Pharmacologica Sinica npg muscle), vascular tissue engineering, and skin tissue engineering, as well as carriers for proteins and DNA [2,4] .…”

Section: Introductionmentioning

confidence: 99%

Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

et al. 2011

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Aim: Bioartificial bone tissue engineering is an increasingly popular technique to solve bone defect challenges. This study aimed to investigate the interactions between matrix composition and appropriate cell type, focusing on hydroxyapatite (HA), to achieve a more effective combination for bone regeneration. Methods: Human unrestricted somatic stem cells (USSCs) were isolated from placental cord blood. The cellular and molecular events during the osteo-induction of USSCs were evaluated for 21 d under the following conditions: (1) in basal culture, (2) supplemented with hydroxyapatite nanoparticle (nHA) suspension, and (3) seeded on electrospun aligned nanofibrous poly-ε-caprolactone/poly-L-lactic acid/nHA (PCL/PLLA/nHA) scaffolds. The scaffolds were characterized using scanning electron microscope (SEM), fourier transform infrared spectroscopy (FTIR) and tensile test. Results: Maintenance of USSCs for 21 d in basal or osteogenic culture resulted in significant increase in osteoblast differentiation. With nHA suspension, even soluble osteo-inductive additives were ineffective, probably due to induced apoptosis of the cells. In contrast to the hindrance of proliferation by nHA suspension, the scaffolds improved cell growth. The scaffolds mimic the nanostructure of natural bone matrix with the combination of PLLA/PCL (organic phase) and HA (inorganic phase) offering a favorable surface topography, which was demonstrated to possess suitable properties for supporting USSCs. Quantitative measurement of osteogenic markers, enzymatic activity and mineralization indicated that the scaffolds did not disturb, but enhanced the osteogenic potential of USSCs. Moreover, the alignment of the fibers led to cell orientation during cell growth. Conclusion:The results demonstrated the synergism of PCL/PLLA/nHA nanofibrous scaffolds and USSCs in the augmentation of osteogenic differentiation. Thus, nHA grafted into PCL/PLLA scaffolds can be a suitable choice for bone tissue regeneration.

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“…Chitosan is partially deacetylated chitin, which is found in nature as a major organic component in several biocomposites, and has a crucial role in the hierarchical control of the biomineralization process. Furthermore, chitosan is one of the most important candidates for bone tissue engineering scaffolds, and possesses better mechanical properties than other natural polymers [6,7]. Therefore, in this study polysaccharide chitosan scaffolds were selected as 545 Rapid biomimetic mineralization of chitosan scaffolds with a precursor sacrificed method in ethanol/water mixed solution the porous matrix for mineralization, which control the nucleation, deposition and growth of the nanometer scaled HAP.…”

Section: Introductionmentioning

confidence: 99%

Rapid biomimetic mineralization of chitosan scaffolds with a precursor sacrificed method in ethanol/water mixed solution

Li¹,

Zhao²,

Ding³

et al. 2011

Express Polym. Lett.

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Abstract. Biomimetic mineralization was performed on a large scale by a rapid and efficient approach. Chitosan scaffolds were placed in a mixed solution of urea, ethanol and distilled water, followed by the introduction of dibasic sodium phosphate (0.1M) and calcium chloride (0.1M) with the molar ratio of 1.67. These mixed solvents was then adjusted to weakly alkaline by adding sodium hydroxide solution. Finally the reaction mixture was sealed and kept at 80ºC for predetermined time. The composition and morphology of the apatite and the hybrid scaffolds were investigated by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier Transform Infrared spectroscopy (FTIR) and environmental scanning electron microscopy (ESEM). The mechanism of nucleation and growth of crystals was discussed as well. The results revealed that chitosan scaffolds improved the crystallinity of hydroxyapatite (HAP) crystals. With the extension of mineralization time, the mineral layers on the outer surface and inner section of chitosan scaffolds increased as well. Furthermore, the compressive strength and modulus of the HAP-chitosan scaffolds biocomposites increased to 0.55±0.003 and 29.29±1.25 MPa respectively. Such one-pot approach may be extended to the mineralization of other materials and will have a broad application in the future.

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In vitro bioactivity of bioresorbable porous polymeric scaffolds incorporating hydroxyapatite microspheres

Cited by 73 publications

References 21 publications

Fabrication and characterization of polycaprolactone/calcium sulfate whisker composites

Fabrication and characterization of polycaprolactone/calcium sulfate whisker composites

Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

Rapid biomimetic mineralization of chitosan scaffolds with a precursor sacrificed method in ethanol/water mixed solution

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