In vitro binding of matrix metalloproteinase‐2 (MMP‐2), MMP‐9, and bacterial collagenase on collagenous wound dressings

Abstract

Chronic wounds are characterized by failure in wound-healing response and a delay in healing or nonclosure of the wounds. This results in a high effort in clinical treatment and/or home care. A major difference between acute wounds and chronic wounds is the imbalance of proteinase inhibitors and proteinase activity that regulates the degradation and regeneration of the extracellular matrix proteins. Collagen and collagen/oxidized regenerated cellulose dressings act as a competitive substrate for matrix metalloproteinase-2, matrix metalloproteinase-9, and bacterial collagenase and influence this imbalance positively. Both wound dressings, approved for chronic wound treatment, the bovine collagen type I sponge and the oxidized regenerated cellulose collagen sponge, did not differ significantly in their sorption profiles for all enzymes. In general, binding was enhanced with a longer incubation time. The density of the device and the accessible surface, which can be controlled by the manufacturing process, are the crucial factors for the efficiency of the wound dressing.

Acute and Impaired Wound Healing

“…Recently, it has been shown that bacterial collagenase (as well as human MMPs) can be bound to collagen or oxidized regenerated cellulose/collagen sponge. 87 This, together with the notion that components of the matrix degraded by bacterial collagenase have stimulatory effects on cells within the wound bed—epithelial, endothelial, inflammatory cells, and fibroblasts—suggest that matrix-based wound dressings may serve to deliver the enzyme into a wound bed.…”

Extracellular Bacterial Proteases in Chronic Wounds: A Potential Therapeutic Target?

“…38 Likewise, the incorporation of collagen-I substrates into wound dressings has been shown to effectively sequester not only the mammalian gelatinases MMP-2 and MMP-9 but also Clostridium histolyticum bacterial collagenase. 39 Photodynamic therapy. Photodynamic therapy (PDT) is the application of a photoactive dye followed by irradiation, which leads to cell death in the presence of oxygen.…”

Role of Single-Dose Clostridiopeptidase A Collagenase in Peritoneal Adhesions

“…The enzyme bound to the collagen fibers. However, because of the dense matrix structure, the reduced surface area, and limited binding sites, the binding of enzyme onto minirods was delayed and thus less pronounced in comparison with porous wound dressings [28]. …”