In vitro atmosphere of cultured C3 and CAM plants in relation to day-lengths.

Doi, Motoaki; Oda, Hisashi; Asahira, Tadashi

doi:10.2525/ecb1963.27.9

Cited by 14 publications

(2 citation statements)

References 5 publications

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“…Multiplying shoots of A. zapota grown on sucrose free medium under ambient CO 2 concentration of growth room showed significant reduction in leaf area, fresh and dry mass. This can be accounted to quick consumption of CO 2 present in the vessel and such cultures starved for CO 2 during large part of photoperiod (Fujiwara et al 1987, Doi et al 1989. Increasing the concentration of CO 2 to 10.0 and 40.0 g m -3 showed improved rate of shoot multiplication.…”

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In vitro Growth and Shoot Multiplication of Achras zapota in a Controlled Carbon Dioxide Environment

Dave¹,

Purohit²

2004

Biol. plant.

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The culture vessels with multiplying shoots of Achras zapota L. on Schenk and Hildebrandt (SH) medium containing 8.88 µM 6-benzylaminopurine (BAP) with or without sucrose were kept under varied CO 2 concentrations ranging from 0.6 to 40.0 g m -3 using different concentrations of sodium bicarbonate (NaHCO 3 ), sodium carbonate (Na 2 CO 3 ), potassium bicarbonate (KHCO 3 ), and potassium carbonate (K 2 CO 3 ) in small acrylic chambers. Complete absence of carbon source caused death of shoots within 20 d. Under elevated concentrations of CO 2 (10.0 and 40.0 g m -3 ) the shoots grew photoautotrophically on sucrose-free medium. The growth of cultures was better at 40.0 g (CO 2 ) m -3 than on 3.0 % sucrose under ambient air of growth room. However, the best response was obtained at 10.0 g (CO 2 ) m -3 and 3.0 % sucrose where maximum number of shoots, shoot length, fresh and dry mass, total number of leaves and leaf area was observed.

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In vitro Growth and Shoot Multiplication of Achras zapota in a Controlled Carbon Dioxide Environment

Dave¹,

Purohit²

2004

Biol. plant.

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“…Lack of both the carbon sources (CO 2 and sucrose) caused gradual deterioration of cultures and subsequent death in SFSM and SFLM due to starvation. 41,42 Improvement in in vitro growth and multiplication was obtained when sucrose-free cultures were grown under a controlled and CO 2 -enriched environment. Low sucrose contents promoted the photosynthetic rate of Quince shoots.…”

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Photosynthetic efficiency and in vitro growth of Celastrus paniculatus Willd. under varied concentrations of CO2

Habibi¹,

Purohit²

2019

Int J Phytocos Nat Ingred

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Introduction: Celastrus paniculatus (family: Celastraceae) is a woody climbing shrub valued for its immense medicinal properties contained in its various plant parts. Over-exploitation and poor natural regeneration either by seed or other method/s have resulted into depleting population of C. paniculatus in natural habitats in India. Novel approaches such as liquid culture media and photoautotrophic multiplication of shoots on sugar-free media has proved useful to obtain photosynthetically active micropropagated plantlets. Methods: The shoot cultures of C. paniculatus were multiplied on sucrose containing and sucrose-free semi-solid and liquid media. The cultures were further incubated under various concentrations of carbon dioxide (0.0 to 40.0 gm-3). The assessment of chlorophyll fluorescence parameters (F o , F m , F' m , F t , F v /F m and ΦPS2) under similar conditions was undertaken. Results: It was observed that CO 2 enrichment favored shoot multiplication and elongation and biomass production in sucrose supplemented medium. CO 2 (10.0 gm-3) along with sucrose (3.0%), recorded maximum growth on semi-solid and liquid media. The valuation of F o , F m , F' m , F t , F v /F m and ΦPS2 revealed that an increase in the concentration of CO 2 resulted in a decline in all the parameters especially the F v / F m and ΦPS2. On the contrary, withdrawal of sucrose from the medium under CO 2 enriched conditions resulted in a moderate growth rate and biomass production. However, F v /F m and ΦPS2 were considerably improved in shoot cultures incubated under elevated concentrations of CO 2 (10.0 gm-3) without sucrose in the medium indicating their photoautotrophic growth. Conclusion: Liquid medium proved to be superior for overall growth and biomass production over its semi-solid counterparts. The observations of photochemical efficiency in shoot cultures grown on liquid medium were at par with their semi-solid counterparts indicating no adverse effects such as hyperhydricity.

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