In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch

Jafri, Laila; Saleem, Shemaila; ul-Haq, Ihsan; Ullah, Najeeb; Mirza, Bushra

doi:10.1016/j.arabjc.2014.05.002

Cited by 99 publications

(74 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The finding is in agreement with previous works [22,23] which showed that ethylacetate fractions contain high amount of phenolics/flavoniods, thus initiating their uses as flavonoid-rich sources. Flavonoids have aroused considerable interest recently because of their potential beneficial effects on human health.…”

Section: Discussionsupporting

confidence: 95%

Ethylacetate extract of red onion (Allium cepa L.) tunic affects hemodynamic parameters in rats

Olayeriju

Olaleye

Crown

et al. 2015

Food Science and Human Wellness

View full text Add to dashboard Cite

The effect of ethylacetate extract of red onion (Allium cepa) tunic (ACTE) on some hemodynamic and biochemical parameters were evaluated in normotensive albino rats.Blood pressure parameters were determined in anaesthetized rats orally administered ACTE (10-, 20-, or 40 mg/kg) or ramipril (1 mg/kg) once daily for 14 days. Respectively, 10-, 20-, or 40 mg/kg ACTE produced significant (P<0.01), dose-dependent fall in systolic blood pressure, SBP (21%, 27%, 33%), diastolic blood pressure, DBP (6%, 10%, 16%), pulse pressure, PP (42%, 49%, 56%), mean arterial blood pressure, MAPB (13%, 18%, 23%) and heart rate, HR (4%, 5%, 7%). The highest effective dose (40 mg/kg) compared well with ramipril (1 mg/kg) with regards to SBP (41%), DBP (19%), PP (70%), MABP (29%) and HR (10%). Similar trends (decreases) were recorded for 40 mg/kg ACTE and ramipril respectively as regards the activities of serum enzymes: creatine kinase (60% and 65%), ALT (18% and 14%) and ALP (28% and 16%). HPLC fingerprints of the flavonoid-rich ACTE revealed that flavonols: quercetin, quercitrin, isoquercitrin, rutin and kaempferol are the active flavonoids. The results demonstrate the hypotensive effect of Allium cepa tunic flavonoids initiating further investigation of their individual or synergistic contribution(s) to the observed effects.

show abstract

Section: Discussionsupporting

confidence: 95%

Ethylacetate extract of red onion (Allium cepa L.) tunic affects hemodynamic parameters in rats

Olayeriju

Olaleye

Crown

et al. 2015

Food Science and Human Wellness

View full text Add to dashboard Cite

show abstract

“…For total flavonoid content determination, aluminum chloride colorimetric method was employed with slight modifications according to system suitability [28]. An aliquot of 20 µL from 4 mg/mL dH2O stock solution of each test sample was transferred in respective well of 96 plates to which 10 µL of 10% aluminum chloride, 10 µL of 1.0 M potassium acetate and 160 µL of distilled water was added.…”

Section: -Determination Of Total Flavonoid Contentmentioning

confidence: 99%

“…Total phenolic content was estimated according to previously described procedure [28] after slight modification. An aliquot of 20 µL from 4 mg/mL dH2O stock solution of each test sample was transferred in respective well of 96 well plate followed by an addition of 90 µL of Folin-Ciocalteu reagent.…”

Section: -Determination Of Total Phenolic Contentmentioning

confidence: 99%

Effect of capping agents: Structural, optical and biological properties of ZnO nanoparticles

Javed

Usman

Tabassum

et al. 2016

Applied Surface Science

137

View full text Add to dashboard Cite

“…We selected H. nepalensis K. Koch, a widely available plant in Pakistan, for study of its anticancer potential on the basis of information gathered from local healers (or hakeems), ethnobotanical survey, and our previous work (Jafri et al ., ; Inayatullah et al ., ). Hamayun et al () has also reported on the folk medicinal use of H. nepalensis to treat cancer.…”

Section: Introductionmentioning

confidence: 99%

Hedera nepalensis K. Koch: A Novel Source of Natural Cancer Chemopreventive and Anticancerous Compounds

Jafri

Saleem

Kondrytuk

et al. 2015

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

Traditional medicinal plants are often used for both the prevention and the treatment of local diseases. Taking into consideration the medicinal importance of Hedera nepalensis within local Pakistani traditions, the present study was undertaken to analyze the in vitro cancer chemopreventive and cytotoxic properties of the plant. The in vitro cancer chemopreventive testing was performed using nitrite assay, NFκB assay, aromatase assay, and quinone reductase 1 (QR1) assay. The cytotoxic potential was evaluated on three cancer-cell lines: MCF-7, MDA-MB-231, and HeLa using sulforhodamine B (SRB) assay. The results of cancer chemopreventive assays show that n-hexane and ethyl acetate fractions of tested plant have promising cancer chemopreventive potential. Lupeol isolated from n-hexane as well as ethyl acetate fraction showed lowest IC50 (0.20 ± 1.9 μM) in NFκB assay. Crude extract and its fractions inhibited the growth of three cancer cell lines by more than 60%, IC50 value of lupeol varied from 2.32 to 10.2 μM. HPLC-DAD-based quantification of lupeol in different plant tissues demonstrated that leaves of H. nepalensis are a rich source of lupeol (0.196 mg/100 mg dry weight). Our data have shown that H. nepalensis harbors cancer chemopreventive and cytotoxic agents.

show abstract

In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch

Cited by 99 publications

References 35 publications

Ethylacetate extract of red onion (Allium cepa L.) tunic affects hemodynamic parameters in rats

Ethylacetate extract of red onion (Allium cepa L.) tunic affects hemodynamic parameters in rats

Effect of capping agents: Structural, optical and biological properties of ZnO nanoparticles

Hedera nepalensis K. Koch: A Novel Source of Natural Cancer Chemopreventive and Anticancerous Compounds

Contact Info

Product

Resources

About