In Vitro Assessment of Antioxidant Effect of Begonia Rex Putz. Leaf Extract on Oxidative Stress Biomarkers In The Equine Erythrocytes Model

Buyun, Lyudmyla; Tkachenko, Halyna; Osadowski, Zbigniew

doi:10.15414/agrobiodiversity.2018.2585-8246.094-110

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…The level of total antioxidant capacity was not-significantly changed after treatment both in equine plasma and erythrocytes (Buyun et al, 2022). Assessment of oxidative stress biomarkers in the equine blood and the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) after in vitro incubation with leaf extract obtained from D. parishii was conducted in our previous study (Buyun et al, 2019(Buyun et al, , 2020.…”

Section: Resultsmentioning

confidence: 99%

Biomarkers of Oxidative Stress in the Equine Erythrocytes after In Vitro Treatment with Extracts Derived from Different Pseudobulbs of Dendrobium parishii RCHB.F. (Orchidaceae) Plants

Buyun

2023

Agrobiodivers Improv Nutr health life Qual

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The current study was conducted to investigate the antioxidant properties of extracts derived from different pseudobulbs of Dendrobium parishii Rchb.f. using biomarkers of oxidative stress (2-thiobarbituric acid reactive substances as biomarkers of lipid peroxidation, aldehydic and ketonic derivatives of oxidative modification of proteins (OMP), total antioxidant capacity (TAC)) in the equine erythrocytes after in vitro treatment with the extracts. The current study is a continuation of our cooperation with M.M. Gryshko National Botanic Garden, National Academy of Science of Ukraine (Kyiv, Ukraine) concerning investigations of antibacterial and antioxidant properties of extracts derived from leaves and pseudobulbs of some species belonging to the Orchidaceae family. The antioxidant properties of extracts derived from different pseudobulbs of D. parishii using biomarkers of oxidative stress in the equine erythrocytes after in vitro treatment with the extracts revealed that extracts derived from different pseudobulbs of D. parishii exhibited varying activity. Extracts derived from the first, second, and third pseudobulbs of D. parishii increased lipid peroxidation after in vitro treatment of equine erythrocytes. Extracts derived from the first, sixth, and seventh pseudobulbs of D. parishii caused to decrease in the levels of aldehydic derivatives of OMP. On the other hand, ketonic derivatives of OMP in the erythrocyte samples after incubation with extracts derived from all parts of pseudobulbs of D. parishii (beginning from the base of the growing tip of the rhizome) were decreased. Moreover, extracts derived from the first and sixth parts of pseudobulbs of D. parishii after incubation with erythrocyte samples caused to increase in the TAC levels. The study of extracts derived from D. parishii supports its favorable biological activities and lays a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilization of extracts of D. parishii.

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Section: Resultsmentioning

confidence: 99%

Biomarkers of Oxidative Stress in the Equine Erythrocytes after In Vitro Treatment with Extracts Derived from Different Pseudobulbs of Dendrobium parishii RCHB.F. (Orchidaceae) Plants

Buyun

2023

Agrobiodivers Improv Nutr health life Qual

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“…In our previous studies, in vitro protective effects of extracts obtained from various tropical and subtropical plants against hemolysis, lipid peroxidation, and oxidative damage of proteins in equine erythrocytes were evaluated Buyun et al, 2018). In case of Ficus genus, we have focused on the evaluation of antioxidant effect of leaf extract obtained from F. religiosa L. based on oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives content of protein oxidative modification (OMP), total antioxidant capacity (TAC)] using the equine erythrocytes model.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antioxidant Activities of Aqueous Extracts Derived from Leaves of Juvenile and Mature Shoots of Ficus Pumila L. (Moraceae)

Tkachenko

Buyun²,

Kurhaluk³

et al. 2019

Agrobiodiversity for Improving of Nutrition, Health and Life Quality

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The aim of this study was to investigate the in vitro antioxidant activity of aqueous extracts from the leaves developed on the shoots of various developmental stages (juvenile and mature/generative) of Ficus pumila L. using the oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives content of protein oxidative modification, total antioxidant capacity] on the equine erythrocytes' suspension model. Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in proportion 1 : 19, w/w). The equine erythrocyte aliquots were used in the study. A volume of 0.1 ml of the F. pumila extract was added to 1.9 ml of clean equine erythrocytes. For positive control (blank), phosphate buffer was used. The treatment with the extract obtained from leaves of mature shoots reduced the erythrocytes TBARS level by 22% (p = 0.029), while TBARS level was increased by 15.5% (p >0.05) when incubated with an extract derived from leaves of juvenile shoots as compared to untreated erythrocytes. When equine erythrocytes were incubated with extract obtained from leaves of mature shoots, the ketonic derivatives level was significantly decreased by 6.9% (p = 0.040), while non-significantly decrease both aldehydic and ketonic derivatives of OMP was observed after incubation with extract derived from juvenile shoots (by 8.18 and 12.5%, p >0.05). The treatment by F. pumila leaf extract (from juvenile and mature shoots) caused the increase of TAC in erythrocyte suspension as compared to untreated erythrocytes. Thus, extracts derived from both juvenile and mature shoots resulted in an increase of total antioxidant capacity of equine erythrocytes' suspension.

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In Vitro Assessment of Antioxidant Effect of Begonia Rex Putz. Leaf Extract on Oxidative Stress Biomarkers In The Equine Erythrocytes Model

Abstract: AGROBIODIVERSITY f o r i m p r o v i n g n u t r i t i o n , h e a l t h a n d l i f e q u a l i t y 2018

Cited by 2 publications

References 24 publications

Biomarkers of Oxidative Stress in the Equine Erythrocytes after In Vitro Treatment with Extracts Derived from Different Pseudobulbs of Dendrobium parishii RCHB.F. (Orchidaceae) Plants

Biomarkers of Oxidative Stress in the Equine Erythrocytes after In Vitro Treatment with Extracts Derived from Different Pseudobulbs of Dendrobium parishii RCHB.F. (Orchidaceae) Plants

In Vitro Antioxidant Activities of Aqueous Extracts Derived from Leaves of Juvenile and Mature Shoots of Ficus Pumila L. (Moraceae)

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