In Vitro Antioxidant Activities of Aqueous Extracts Derived from Leaves of Juvenile and Mature Shoots of Ficus Pumila L. (Moraceae)

Tkachenko, Halyna; Buyun, Lyudmyla; Kurhaluk, Natalia; Honcharenko, Vitaliy; Prokopiv, Andriy; Osadowski, Zbigniew

doi:10.15414/agrobiodiversity.2019.2585-8246.001-013

Cited by 4 publications

(9 citation statements)

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“…The treatment by F. pumila leaf extract (from juvenile and mature shoots) caused the increase of TAC in erythrocyte suspension as compared to untreated erythrocytes. Thus, extracts derived from both juvenile and mature shoots increased the total antioxidant capacity of equine erythrocytes (Tkachenko et al, 2019).…”

Section: Introductionmentioning

confidence: 89%

“…In our previous study (Tkachenko et al, 2018(Tkachenko et al, , 2019, we highlight the antioxidant potential of an aqueous extract derived from leaves of other Ficus species using an equine erythrocyte suspension. In the study (Tkachenko et al, 2018), we have focused on the antioxidant effect of an extract derived from leaves of F. religiosa L. on oxidative stress biomarkers (2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives of protein oxidative modification (OMP), total antioxidant capacity (TAC)) using the model of equine erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

“…Later, we investigated the in vitro antioxidant activity of aqueous extracts derived from the leaves developed on the shoots of various developmental stages (juvenile and mature/generative) of F. pumila L. using the oxidative stress biomarkers (TBARS, carbonyl derivatives of protein oxidative modification, total antioxidant capacity) on the model of equine erythrocyte suspension (Tkachenko et al, 2019). The treatment with the extract derived from leaves of mature shoots reduced the erythrocyte's TBARS level by 22% (p = 0.029), while the TBARS level was increased by 15.5% (p >0.05) when incubated with an extract derived from leaves of juvenile shoots as compared to untreated erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Antioxidant Response of the Equine Blood Treated by Leaf Extract of Ficus drupacea Thunb.

Tkachenko

2022

Agrobiodivers Improv Nutr health life Qual

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The present study aimed to evaluate the antioxidant potential of the aqueous extract derived from the leaves of Ficus drupacea Thunb. using oxidative stress biomarkers (2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity) and antioxidant defences (activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase GPx), ceruloplasmin (CP)) on the model of equine erythrocytes and plasma after incubation in vitro. Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1 M phosphate buffer (pH 7.4) (in the proportion of 1:19, w/w). The equine erythrocytes and plasma were used in the current study. A volume of 0.1 ml of the F. drupacea extract was added to 1.9 ml of equine erythrocytes or plasma. For positive control (blank), 0.1 ml of phosphate buffer was used. The treatment of equine plasma and erythrocytes by extract derived from leaves of F. drupacea resulted in reduced lipid peroxidation and oxidatively modified protein. Treatment by extract resulted in a reduced erythrocyte TBARS level of 21.9% (p = 0.017) compared to the untreated samples. The levels of aldehydic and ketonic derivatives of oxidatively modified proteins were non-significantly decreased. The incubation of equine plasma with an extract derived from leaves of F. drupacea increased antioxidant defences. The activity of SOD and GPx were increased by 41.6% (p = 0.000) and 61.5% (p = 0.000) in the equine plasma after in vitro incubation with an extract derived from leaves of F. drupacea compared to the untreated samples. The level of total antioxidant capacity was non-significantly increased. However, further detailed investigation, especially in vivo and in vitro antioxidant studies is needed to justify the use of extract derived from leaves of F. drupacea as a natural source of antioxidants.

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Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro Antioxidant Response of the Equine Blood Treated by Leaf Extract of Ficus drupacea Thunb.

Tkachenko

2022

Agrobiodivers Improv Nutr health life Qual

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“…In our previous studies, we assessed in vitro antioxidant activities of aqueous extracts derived from leaves of juvenile and mature shoots of Ficus pumila L. [32] and leaf extract of Ficus drupacea Thunb. using equine blood as a biological model for cytotoxic studies [31].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

Tkachenko¹,

Kurhaluk²,

Buyun³

et al. 2023

BHT

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Purpose: to investigate the antioxidant properties of the aqueous extracts derived from leaves of Ficus deltoidea using the model of human blood. For this purpose, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), content of aldehydic and ketonic derivatives of oxidatively modified proteins, total antioxidant capacity (TAC)] in the human erythrocytes after in vitro incubation with aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) were used. Resistance of human erythrocytes after in vitro treatment by aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) was evaluated by HCl-induced hemolysis using a percentage of hemolyzed erythrocytes per each 30 sec. and a total time of hemolysis. Methodology. The leaves of F. deltoidea were collected in M.M. Gryshko National Botanic Garden (Kyiv, Ukraine). Blood (10-20 mL) was obtained from normal volunteers via venipuncture (4 males and 5 females aged 28-53 years old). An erythrocyte suspension at 1% hematocrit was incubated with 4 mM phosphate buffer (pH 7.4) (control) and pre-incubated with the extract of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL, respectively) at 37 °C for 60 min. The level of lipid peroxidation was determined by quantifying the concentration of 2-thiobarbituric acid reacting substances (TBARS). The rate of protein oxidative destruction was estimated from the reaction of the resultant carbonyl derivatives of amino acid reaction with 2,4-dinitrophenylhydrazine (DNFH). The total antioxidant capacity (TAC) in the samples was estimated by measuring the TBARS level after Tween 80 oxidation. The HCl-induced resistance of erythrocytes was measured spectrophotometrically with 0.1M HCl. The significance of differences (significance level, p < 0.05) was examined using the Mann-Whitney U test. Scientific novelty. The treatment of human erythrocytes by extract derived from leaves of F. deltoidea at the final concentration of 5 mg/mL resulted in an increase of TBARS as biomarkers of lipid peroxidation and aldehydic derivatives of oxidatively modified proteins with a simultaneous decrease in the total antioxidant capacity compared to the untreated samples. On the other hand, in vitro treatment of human erythrocytes with an extract derived from leaves of F. deltoidea at the final concentration of 0.5 mg/mL resulted in the same values of TBARS level, aldehydic and ketonic derivatives of OMP, and total antioxidant capacity as the untreated samples. Extract of F. deltoidea at the final concentrations of 5 mg/mL and 0.5 mg/mL possess hemolytic properties to human erythrocyte suspension after 1-h incubation in vitro. Conclusions. The changes in the oxidative stress biomarkers using the in vitro model of human erythrocytes to evaluate the antioxidant activities of the aqueous extract derived from the leaves of F. deltoidea at the two final concentrations (5 mg/mL and 0.5 mg/mL) revealed that high concentration of extract (5 mg/mL) resulted in the increase of lipid peroxidation and protein oxidation with a simultaneous decline in the total antioxidant capacity. HCl-induced hemolysis was activated after the treatment by extract derived from the leaves of F. deltoidea at the two final concentrations. More studies are warranted in the future, to illustrate the potential and mechanisms of F. deltoidea in preventing oxidative stress using different cell models in vitro and different final concentrations of the extract. Also, further studies are warranted to identify the bioactive components that contribute to this protective effect.

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“…In our previous study [33,34], we highlight the antioxidant potential of an aqueous extract derived from leaves of other Ficus species using an equine erythrocyte suspension. In the study [34], we have focused on the antioxidant effect of extract derived from leaves of F. religiosa L. on oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), carbonyl derivatives of protein oxidative modification (OMP), total antioxidant capacity (TAC)] using the model of equine erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antioxidant Response of the Equine Blood Treated by Extract Derived From Leaves of Ficus Deltoidea Jack (Moraceae)

H.,

N.,

et al. 2022

Prirodničij alʹmanah. Serìâ: Bìologìčnì nauki

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Ficus deltoidea Jack, which has strong antioxidant properties, is widely consumed in traditional medicine as a treatment for various diseases. The current study aimed to investigate the oxidative stress biomarkers, such as 2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity, as well as antioxidant defenses (activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), ceruloplasmin (CP)) in the equine erythrocytes and plasma to evaluate the antioxidant activities of the aqueous extract derived from leaves of Ficus deltoidea. The leaves of F. deltoidea were collected at M.M. Gryshko National Botanic Garden (Kyiv, Ukraine) and the Botanic Garden of Ivan Franko National University in Lviv (Lviv, Ukraine). Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in the proportion of 1:19, w/w) at room temperature. The extracts were then filtered and used for analysis. A volume of 0.1 mL of the plant extract was added to 1.9 mL of clean equine erythrocytes or plasma (the final concentration of the extract was 5 mg/mL). For positive control, 0.1 mL of phosphate buffer (pH 7.4) was used. The treatment of equine plasma and erythrocytes by extract derived from leaves of F. deltoidea resulted in reduced carbonyl derivatives of the oxidatively modified protein. The levels of lipid peroxidation were non-significantly changed. The incubation of equine plasma with an extract derived from leaves of F. deltoidea resulted in an increase in the activity of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase with a simultaneous decrease of ceruloplasmin level. The level of total antioxidant capacity was non-significantly increased. The results obtained suggested the antioxidant activity of the extract derived from the leaves of F. deltoidea plants exhibiting a decrease in the biomarkers of oxidative stress and enhancement of antioxidant defenses in the equine erythrocytes and plasma after in vitro treatment with the extract. However, further detailed investigation, especially in vivo and in vitro antioxidant studies is needed to justify the use of extract derived from leaves of F. deltoidea as a natural source of antioxidants.

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In Vitro Antioxidant Activities of Aqueous Extracts Derived from Leaves of Juvenile and Mature Shoots of Ficus Pumila L. (Moraceae)

Cited by 4 publications

References 19 publications

In vitro Antioxidant Response of the Equine Blood Treated by Leaf Extract of Ficus drupacea Thunb.

In vitro Antioxidant Response of the Equine Blood Treated by Leaf Extract of Ficus drupacea Thunb.

In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

In Vitro Antioxidant Response of the Equine Blood Treated by Extract Derived From Leaves of Ficus Deltoidea Jack (Moraceae)

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