In vitro assessment of Ag and TiO2 nanoparticles cytotoxicity

Ganapathi, Apoorva Priyanka; Devaki, Ramakrishna; Reddy, Thuniki Naveen; Manna, Joydeb; Tirumuru, Bhaskar; Gopu, Chinnapu Reddy; Deepthi, S.; Trivedi, Rajiv; Rana, Rohit; Hasan, Annie

doi:10.5455/2320-6012.ijrms20141122

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…Although nanoparticles have antimicrobial activity, however, silver and titanium dioxide nanoparticles are also known to induce oxidative stress in vitro and in vivo (53). In our study, the plant extract of E. prostrata and synthesized Ag NPs were found to possess maximum antioxidant activity.…”

Section: Discussionmentioning

confidence: 69%

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G ₀ /G ₁ Arrest followed by Necrotic Cell Death in Leishmania donovani

Zahir¹,

Chauhan

Bagavan³

et al. 2015

Antimicrob Agents Chemother

154

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The aim of the present study was to synthesize silver (Ag) and titanium dioxide (TiO 2 ) nanoparticles (NPs) using green synthesis from aqueous leaf extract of Euphorbia prostrata as antileishmanial agents and to explore the underlying molecular mechanism of induced cell death. In vitro antileishmanial activity of synthesized NPs was tested against promastigotes of Leishmania donovani by alamarBlue and propidium iodide uptake assays. Antileishmanial activity of synthesized NPs on intracellular amastigotes was assessed by Giemsa staining. The leishmanicidal effect of synthesized Ag NPs was further confirmed by DNA fragmentation assay and by cell cycle progression and transmission electron microscopy (TEM) of the treated parasites. TEM analysis of the synthesized Ag NPs showed a spherical shape with an average size of 12.82 ؎ 2.50 nm, and in comparison to synthesized TiO 2 NPs, synthesized Ag NPs were found to be most active against Leishmania parasites after 24 h exposure, with 50% inhibitory concentrations (IC 50 ) of 14.94 g/ml and 3.89 g/ml in promastigotes and intracellular amastigotes, respectively. A significant increase in G 0 /G 1 phase of the cell cycle with a subsequent decrease in S (synthesis) and G 2 /M phases compared to controls was observed. The growth-inhibitory effect of synthesized Ag NPs was attributed to increased length of S phase. A decreased reactive oxygen species level was also observed, which could be responsible for the caspase-independent shift from apoptosis (G 0 /G 1 arrest) to massive necrosis. High-molecular-weight DNA fragmentation as a positive consequence of necrotic cell death was also visualized. We also report that the unique trypanothione/trypanothione reductase (TR) system of Leishmania cells was significantly inhibited by synthesized Ag NPs. The green-synthesized Ag NPs may provide promising leads for the development of costeffective and safer alternative treatment against visceral leishmaniasis.

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Section: Discussionmentioning

confidence: 69%

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G ₀ /G ₁ Arrest followed by Necrotic Cell Death in Leishmania donovani

Zahir¹,

Chauhan

Bagavan³

et al. 2015

Antimicrob Agents Chemother

154

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“…Other studies aimed at comparison of pure TiO 2 nanoparticles to separately prepared nanoparticles of different composition. Ganapathi analyzed amounts of Glutathione (GSH), Nitric Oxide (NO), and superoxide dismutase (SOD) which were estimated by ELISA method as a result of TiO 2 and Ag exposure to human plasma. Authors observed that silver and titanium dioxide nanoparticles exhibited nanoparticles‐mediated cytotoxicity by induction of Reactive Oxygen Species (ROS).…”

Section: Introductionmentioning

confidence: 99%

“…This is mainly due to the fact that the TiO 2 ‐based nanocomposite particles analyzed for toxicity were prepared using different methods and techniques . In other studies, the nanocomposite systems were not synthesized and the TiO 2 nanoparticles were compared to separately prepared other types of nanoparticles . It is, however, well known that different synthesis methods result in different properties of the obtained materials .…”

Section: Introductionmentioning

confidence: 99%

The toxicity in vitro of titanium dioxide nanoparticles modified with noble metals on mammalian cells

Rosłon

Jastrzębska

Sitarz

et al. 2018

Int J Applied Ceramic Tech

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The aim of the presented work was to evaluate the effect of modification of titanium dioxide nanoparticles with noble metals and their oxides on selected mammalian cells. The in vitro cytotoxicity studies of titanium dioxide (TiO 2 ) nanoparticles modified with Au, Ag, Pd, Ag 2 O, and PdO with reference to the unmodified TiO 2 nanoparticles were presented. The evaluation of cytotoxic activity of the tested nanocomposite particles was carried out using three cell lines: Caco-2 colorectal epithelial adenocarcinoma cells, BJ normal human skin fibroblasts, and L929 mouse fibroblasts. The in vitro studies included determination of cell viability after 24 and 48 hours of exposure to the nanocomposite particles, using the MTT assay, as well as flow cytometry with Annexin V-FITC staining.Our results indicate that irrespectively of the kind of cell line and assay used, nanoparticles of unmodified titanium dioxide as well as those with addition of gold and palladium have a slight impact on cell viability at the investigated concentration range (10-200 μg/mL). Nanoparticles with addition of silver (Ag and Ag 2 O), were found to have significantly higher toxic effect, the level of which varied depending on the cell line and assay used.

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“…Both have common manufacturing applications such as in cosmetics, water filters, air conditioning filters and food industry. Medically they are both used as antifungal, antimicrobial and in treatment of cancer [19] .…”

Section: Discussionmentioning

confidence: 99%

“…According to previous authors [19] silver (Ag) and titanium dioxide (TiO2) nanoparticles are well-known nanoproducts. Both have common manufacturing applications such as in cosmetics, water filters, air conditioning filters and food industry.…”

Section: Introductionmentioning

confidence: 99%

CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study

Gawish

Shaban

Aal

et al. 2019

Journal of Medical Histology

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Background: Silver nanoparticles (Ag-Nps) and Titanium dioxide nanoparticles (TiO2-Nps) are well-known nanoproducts. Both of them have many industrial applications. Lung is one of the major target organs for prolonged nanoparticles exposure. Objective: This study was designed to investigate and compare the possible histopathological toxic effect of Ag-Nps & TiO2-Nps on lung and which of them is safer for future using. Materials & methods: 54 adult male albino rats were divided into 3 equal groups; GroupI (control group), Group II that subdivided into two subgroups; Subgroup IIa: Ag-Nps group with (100 mg/kg/day) daily for 4 successive weeks and Subgroup IIb recovery group left for 4 weeks without injection, Group III that subdivided into two subgroups; Subgroup IIIa: TiO2-Nps group with (150 mg/kg/day) for 4 successive weeks and Subgroup IIIb recovery group left for 4 weeks without injection. Lung specimens were processed for light and electron microscope and immunohistochemical expression of caspase-3 and CD-68. Morphometric and statistical analysis were performed. Results: Group IIa showed collapsed alveoli, others showed ballooned distension and marked thickening of inter-alveolar septa. Extensive cellular infiltration was detected. Group IIIa showed focal areas of collapsed alveoli and thick inter-alveolar septa. Mild cellular infiltrations were observed. Areas of extravasation were detected in the interstitium. Group IIIb showed signs of improvement which is more than group IIb. Conclusion: exposure to Ag-Nps showed marked alterations on histological structure of lung, which is more than the alteration caused by TiO2-Nps; in addition, recovery period was proved to ameliorate these changes better in TiO2-Nps.

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In vitro assessment of Ag and TiO2 nanoparticles cytotoxicity

Cited by 8 publications

References 26 publications

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G ₀ /G ₁ Arrest followed by Necrotic Cell Death in Leishmania donovani

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G ₀ /G ₁ Arrest followed by Necrotic Cell Death in Leishmania donovani

The toxicity in vitro of titanium dioxide nanoparticles modified with noble metals on mammalian cells

CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study

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In vitro assessment of Ag and TiO2 nanoparticles cytotoxicity

Cited by 8 publications

References 26 publications

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G 0 /G 1 Arrest followed by Necrotic Cell Death in Leishmania donovani

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G 0 /G 1 Arrest followed by Necrotic Cell Death in Leishmania donovani

The toxicity in vitro of titanium dioxide nanoparticles modified with noble metals on mammalian cells

CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study

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Product

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Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G ₀ /G ₁ Arrest followed by Necrotic Cell Death in Leishmania donovani

Green Synthesis of Silver and Titanium Dioxide Nanoparticles Using Euphorbia prostrata Extract Shows Shift from Apoptosis to G ₀ /G ₁ Arrest followed by Necrotic Cell Death in Leishmania donovani