The present paper describes an in vitro assessment of the antibacterial properties and cytotoxic activity on mammalian cells of silver nanoparticles incorporated into an aluminium nano-oxide substrate produced by the thermal decomposition-reduction method. The Al 2 O 3 -Ag nanopowders show good bactericidal and fungicidal properties. An Al 2 O 3 -Ag (12?55 wt-%) specimen at the concentration of 50 mg mL 21 reduced considerably the populations of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis species, whereas the same nanopowder in a concentration of 90 mg mL 21 inhibited significantly the growth of P. aeruginosa, E. coli, S. aureus and Candida albicans and also significantly limited the number of B. subtilis. The Al 2 O 3 -Ag nanoparticles also showed no cytotoxic effects on the three selected cell lines, i.e. L929, BJ and HeLa, and the cells remained .75% viability, as measured by MTT assay, and .102% viability, obtained by EZ4U assay, relative to control at concentration as high as 200 mg mL 21 .

The aim of the presented work was to evaluate the effect of modification of titanium dioxide nanoparticles with noble metals and their oxides on selected mammalian cells. The in vitro cytotoxicity studies of titanium dioxide (TiO 2 ) nanoparticles modified with Au, Ag, Pd, Ag 2 O, and PdO with reference to the unmodified TiO 2 nanoparticles were presented. The evaluation of cytotoxic activity of the tested nanocomposite particles was carried out using three cell lines: Caco-2 colorectal epithelial adenocarcinoma cells, BJ normal human skin fibroblasts, and L929 mouse fibroblasts. The in vitro studies included determination of cell viability after 24 and 48 hours of exposure to the nanocomposite particles, using the MTT assay, as well as flow cytometry with Annexin V-FITC staining.Our results indicate that irrespectively of the kind of cell line and assay used, nanoparticles of unmodified titanium dioxide as well as those with addition of gold and palladium have a slight impact on cell viability at the investigated concentration range (10-200 μg/mL). Nanoparticles with addition of silver (Ag and Ag 2 O), were found to have significantly higher toxic effect, the level of which varied depending on the cell line and assay used.

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Background. Oxidative stress accompanies neurodegeneration and also causes abnormalities in thiamine-dependent processes. These processes have been reported to be diminished in the brains of patients with several neurodegenerative diseases.

The method of using high-performance liquid chromatography with a charged aerosol detector method (HPLC-CAD) was developed for the separation and determination of phospholipids isolated from cell membranes. The established cell lines—normal and neoplastic prostate cells and normal skin fibroblasts and melanoma cells—were selected for the study. Chromatographic separation was performed in the diol stationary phase using a gradient elution based on a mixture of n-hexane, isopropanol and water with the addition of triethylamine and acetic acid as buffer additives. Taking the elements of the Folch and Bligh–Dyer methods, an improved procedure for lipid isolation from biological material was devised. Ultrasound-assisted extraction included three extraction steps and changed the composition of the extraction solvent, which led to higher recovery of the tested phospholipids. This method was validated by assessing the analytical range, precision, intermediate precision and accuracy. The analytical range was adjusted to the expected concentrations in cell extracts of various origins (from 40 µg/mL for PS up to 10 mg/mL for PC). Both precision and intermediate precision were at a similar level and ranged from 3.5% to 9.0%. The recovery for all determined phospholipids was found to be between 95% and 110%. The robustness of the method in terms of the use of equivalent columns was also confirmed. Due to the curvilinear response of CAD, the quantification was based on an internal standard method combined with a power function transformation of the normalized peak areas, allowing the linearization of the signal with an R2 greater than 0.996. The developed method was applied for the isolation and determination of glycerophospholipids from cell membranes, showing that the profile of the tested substances was characteristic of various types of cells. This method can be used to assess changes in metabolism between normal cells and neoplastic cells or cells with certain pathologies or genetic changes.

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