In vitro assessment of acetic-acid-soluble proteins (glutenin) toxicity in celiac disease

Vincenzi, Massimo De; Luchetti, R.; Peruffo, A. Dal Belin; Curioni, Andrea; Pogna, N. E.; Gasbarrini, Giovanni

doi:10.1002/(sici)1522-7146(1996)11:4<205::aid-jbt7>3.0.co;2-o

Cited by 17 publications

(9 citation statements)

References 17 publications

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“…In contrast, the results obtained here strongly suggest that spelt wheat exert toxic effects on K562(S) cells, its toxicity being similar to that of bread wheat. As mentioned above, proteins or peptides from bread wheat, rye and barley, which are harmful to celiac patients, were found to possess the exclusive property of causing agglutination of K562(S) cells 6,8,27 …”

Section: Discussionmentioning

confidence: 98%

Environmental factors of celiac disease: Cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta

Vincentini

Maialetti

Gazza

et al. 2007

J of Gastro and Hepatol

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Section: Discussionmentioning

confidence: 98%

Environmental factors of celiac disease: Cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta

Vincentini

Maialetti

Gazza

et al. 2007

J of Gastro and Hepatol

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“…The prolamines of wheat contain a high content of glutamine ( > 30%) and proline ( > 15%), and the nontoxic prolamines of rice and maize have a low content. The prolamines of oats have an intermediate amino acid composition (Cornell et al, 1992;Wieser, 1995;De Vincenzi et al, 1996). Large quantities of oats are therefore suggested to be harmful (Thompson, 1997;Schmitz, 1997).…”

Section: Discussionmentioning

confidence: 99%

Adult coeliac patients do tolerate large amounts of oats

et al. 2003

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Objective: The aim of the present study was to investigate whether adult patients with coeliac disease in remission could include large amounts of oats in their daily gluten-free diet for an extended period of time without adverse effects. Design, subjects and methods: Twenty adult coeliac patients in remission included large amounts of uncontaminated rolled oats in their daily diet for a prolonged period. The examinations, performed four times during the study period, included small bowel endoscopy with biopsies, blood samples (nutritional status, serological analysis), height and body weight, gastrointestinal symptoms and dietary records. Gastrointestinal symptoms and diet were also investigated through unannounced telephone interviews once a month during the study period. Results: No adverse effects of a large intake of oats were seen in small bowel histology, serology nor in nutritional status in the 15 subjects who completed the whole study period. Two of the subjects dropped out because of gastrointestinal symptoms and three for non-medical reasons. The median intake of oats was 93 g=day and the compliance to the oat diet was found to be good. Examinations of the patients after drop-out did not show any deterioration in small bowel histology or nutritional status nor raised levels of antibodies. Conclusion: Results from this study indicate that adult patients with coeliac disease in remission can include large amounts of controlled wheat-free rolled oats for an extended period of time without adverse effects.

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“…Moreover, a/b-gliadins, g-gliadins and glutenin subunits were shown to agglutinate human myelogenous leukemia K562(S) cells, an exclusive property of grain proteins harmful for celiac patients (De Vincenzi et al 1995, 1996a, 1996b, and stimulate T cell clones derived from jejunal mucosa or peripheral blood of celiac individuals (van de Wal et al 1999;Shan et al 2002;Vader et al 2002;Gianfrani et al 2003). In addition, a few peptides were shown to elicit a strong and rapid T cells response in nearly all celiac patients, these immunodominant sequences occurring in a-or g-gliadins (Sjostrom et al 1998;van de Wal et al 1998;Shan et al 2002).…”

Section: Introductionmentioning

confidence: 89%

“…PT-digested prolamins from bread wheat, rye and barley were found to possess the exclusive property of driving agglutination of the K562(S) cells (De Vincenzi et al 1995, 1996a, 1996b. Here, the minimal agglutinating concentration (MAC) of PT-digested prolamins from both spelt and common wheat required to agglutinate 100% of K562(S) cells was 73 mg/l ( Table 2).…”

Section: Agglutination Of K562(s) Cellsmentioning

confidence: 99%

Variation in noxiousness of different wheat species for celiac patients

Pogna

Gazza

Vincentini

et al. 2008

Journal of Plant Interactions

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Peptic-tryptic (PT) digested prolamins from spelt wheat Triticum aestivum ssp. spelta and landraces ERSA 6 and ERSA 8 of farro wheat T. turgidum ssp. dicoccum were found to agglutinate K562(S) cells, and exert strong toxic effects on Caco-2/TC7 cells. Cytotoxicity of spelt prolamins against Caco-2/TC7 cells was greatly reduced by 10-mer peptide QQPQDAVQPF. By contrast, the PT digests from monoccum wheat (Triticum monococcum) and farro landraces Prometeo, L5563, L5540 and L5558 did not exhibit any negative effects on K562(S) and Caco-2/ TC7 cells. Toxic genotypes ERSA 6 and ERSA 8 were found to share the same gliadin pattern, which was absent in inactive landraces. Monococcum, farro and spelt wheats differed from each other in their responses to antibodies specific for 13-mer cytotoxic sequence FPGQQQPFPPQQP and 10-mer peptide QQPQDAVQPF. This latter sequence was found to occur in high amounts in common wheat line FG, Phaseoulus vulgaris, Ph. coccineus and Lens culinaria.

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In vitro assessment of acetic-acid-soluble proteins (glutenin) toxicity in celiac disease

Cited by 17 publications

References 17 publications

Environmental factors of celiac disease: Cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta

Environmental factors of celiac disease: Cytotoxicity of hulled wheat species Triticum monococcum, T. turgidum ssp. dicoccum and T. aestivum ssp. spelta

Adult coeliac patients do tolerate large amounts of oats

Variation in noxiousness of different wheat species for celiac patients

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