In vitro Assay of Alpha-Glucosidase Inhibitor Activities of Three Seagrasses from Banten Bay, Indonesia

Abstract

Introduction: Diabetes mellitus (DM) is an endocrine disorder characterized by high blood glucose levels. One of the approaches for treating DM is by regulating the postprandial blood glucose via inhibition of α-glucosidase enzymes. Seagrass is known as a plant containing bioactive compounds, especially for therapy antidiabetic.This research aims to evaluate the α-glucosidase inhibition activity of three species seagrasses (Enhalus acoroides, Thalassia hemprichii and Cymodocea rotundata) from the Banten Bay, Indonesia. Methods: The extracts of all parts (root, leaf, and stem) from three seagrasses were prepared with maceration method using 80% ethanol solvents. The α-glucosidase inhibitory activity was conducted by microplate reader at 400 nm using acarbose as a positive control. Furthermore, the kinetics of α-glucosidase inhibition, phytochemical screening, and total phenolics content were evaluated against extract which has the most potential α-glucosidase inhibition. Results: The result showed that the 80% ethanol extracts of Enhalus acoroides (IC 50 values 168.15 ± 2.71 µg/mL) had the most potential α-glucosidase inhibitors activity compared with the positive control acarbose, Thalassia hemprichii and Cymodocea rotundata (IC 50 values 197.27 ± 3.07 µg/mL, 425.86 ± 5.15 µg/mL and 429.28 ± 8.89 µg/mL). The kinetic type of inhibition against α-glucosidase was noncompetitive inhibition. The phytochemical compounds were phenols, flavonoid, terpenes, and tannin with the total phenolic content was 28.76 ± 2.46 mgGAE/g. Conclusion:Enhalus acoroides has the strongest inhibitor of α-glucosidase and can be further developed for DM therapy agents.

Molecular Networking Leveraging the Secondary Metabolomes Space of Halophila stipulaceae (Forsk.) Aschers. and Thalassia hemprichii (Ehrenb. ex Solms) Asch. in Tandem with Their Chemosystematics and Antidiabetic Potentials

“…Both seagrasses showed comparable enzymatic inhibitions, highlighting their potential as antidiabetic agents to surveil the calorie intake ( Supplementary Table S3 ). These findings were consistent with the previous dose-dependent inhibition results attained with H. beccarii [ 46 ] and Th [ 47 ].…”

A mini review on biological activities of genus Thalassia: A marine seagrass

“…The α-glucosidase inhibitory activity was conducted using microplate reader at 400 nm and acarbose was used as a positive control. The research indicated that ethanolic extract of T. hemprichii has a moderate inhibitory action against α-glucosidase and with an IC50 of 425.86±5.15 μg/mL [28] .…”

Evaluation of phytochemical and superoxide dismutase activities of Enhalus acoroides (L.f.) Royle from coastal waters of North Sulawesi, Indonesia

“…In traditional medicine, Enhalus acoroides (L.f.) Royle has been used for various repair purposes, for example, for the treatment of fever and skin diseases [8], muscle aches [9], wounds [10], stomach problems [11], drugs for stings from various types of rays [12], and as sedatives for babies [13]. E. acoroides (L.f.) Royle produces antimicrobial compounds that can act to reduce or control microbial growth and many studies describe antibacterial [14], antifungal [15], antiviral [16], anti-inflammatory [17], antidiabetic [18], and antioxidant [19] activities. Seagrasses, including E. acoroides Available at www.veterinaryworld.org/Vol.13/April-2020/10.pdf (L.f.) Royle, are easily found in Indonesia; the species can be easily identified because it has distinct morphologies.…”