In vitro antiviral activity of antimicrobial peptides against herpes simplex virus 1, adenovirus, and rotavirus

Carriel-Gomes, Márcia Cristina; Kratz, Jadel M.; Barracco, Margherita Anna; Bachère, Evelyne; Barardi, Célia Regina Monte; Simões, Cláudia Maria Oliveira

doi:10.1590/s0074-02762007005000028

Cited by 78 publications

(42 citation statements)

References 25 publications

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“…Furthermore, clavanin A treatment showed lower cytotoxicity on in vitro assays for L929 cells and SPCs, being significantly less toxic than LL-37, and in acute toxicity tests, no adverse reactions were observed at any of the concentrations tested here (data not shown). These data corroborate the previous studies where clavanin A showed low cytotoxicity in human cell erythrocytes and HEp-2 as well as monkey cells of the Vero and MA104 lines (30). Normally, cathelicidins are highly toxic to eukaryotic cells (29).…”

Section: Discussionsupporting

confidence: 91%

Clavanin A Improves Outcome of Complications from Different Bacterial Infections

Silva

Fensterseifer

Rodrigues

et al. 2015

Antimicrob Agents Chemother

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The rapid increase in the incidence of multidrug-resistant infections today has led to enormous interest in antimicrobial peptides (AMPs) as suitable compounds for developing unusual antibiotics. In this study, clavanin A, an antimicrobial peptide previously isolated from the marine tunicate Styela clava, was selected as a purposeful molecule that could be used in controlling infection and further synthesized. Clavanin A was in vitro evaluated against Staphylococcus aureus and Escherichia coli as well as toward L929 mouse fibroblasts and skin primary cells (SPCs). Moreover, this peptide was challenged here in an in vivo wound and sepsis model, and the immune response was also analyzed. Despite displaying clear in vitro antimicrobial activity toward Gram-positive and -negative bacteria, clavanin A showed no cytotoxic activities against mammalian cells, and in acute toxicity tests, no adverse reaction was observed at any of the concentrations. Moreover, clavanin A significantly reduced the S. aureus CFU in an experimental wound model. This peptide also reduced the mortality of mice infected with E. coli and S. aureus by 80% compared with that of control animals (treated with phosphate-buffered saline [PBS]): these data suggest that clavanin A prevents the start of sepsis and thereby reduces mortality. These data suggest that clavanin A is an AMP that could improve the development of novel peptide-based strategies for the treatment of wound and sepsis infections.

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Section: Discussionsupporting

confidence: 91%

Clavanin A Improves Outcome of Complications from Different Bacterial Infections

Silva

Fensterseifer

Rodrigues

et al. 2015

Antimicrob Agents Chemother

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“…3 (continued) Penhrp also proved to be more effective in the prevention of TMV infection of tobacco. In addition to the antimicrobial ability, Penaeidin exhibited a promising antiviral effect against virus (Carriel-Gomes et al 2007;Lofgren et al 2004). It has been previously reported that Penaeidin have a linear proline-rich N-terminal fragment and a cyclic C-terminal fragment with three disulfide bonds.…”

Section: Discussionmentioning

confidence: 99%

The fusion protein Penhrp containing Penaeidin-2 and HarpinZ induced a stronger hypersensitive response and systemic acquired resistance in tobacco comparing to the original HarpinZ

Han

Meng

et al. 2011

Australasian Plant Pathol.

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In the present study, the gene (pen2) encoding a mature Penaeidin-2 from Penaeus vannamei. shrimp and gene (rHarpinZ) encoding a HarpinZ from Pseudomonas syringae pv. tomato were respectively cloned. The pen2 was fused to rHarpinZ with a linker (GGSGSGGSSRGGSGS) and then cloned into pET-32a (+) formed the expression plasmid pET32a (+)/pen-hrp. Penhrp was expressed in the prokaryotic (E. coli Origami (DE3) pLysS) expression system. Functional analysis showed that the fusion protein Penhrp could elicit stronger hypersensitive response (HR) including higher Peroxidase (POD) and Superoxide dismutase (SOD) activities and the transcription of PR-5dB gene than the original HarpinZ. In addition, a stronger systemic acquired resistance (SAR) to TMV in Xanthi nc. tobacco was also induced. These may offer a potential effective strategy for plant disease control.

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“…Molluscs have been found to have broad antiviral activity against viruses from different families. For example, oyster hemolymph has been shown to contain compounds active against T3 coliphage (family Podoviridae) (49), herpes simplex virus type 1 (Herpesviridae), infectious pancreatic necrosis virus (Birnaviridae) (35), and human adenovirus type 5 (Adenoviridae) (50). The means by which these species have come to have antiviral mechanisms that are sufficiently universal to interfere with human viral systems remain open for speculation.…”

Section: Structures and Mechanisms Of Action Of Molluscan Antiviral Cmentioning

confidence: 99%

Marine Snails and Slugs: a Great Place To Look for Antiviral Drugs

Vinh

Benkendorff

Green

et al. 2015

J Virol

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e Molluscs, comprising one of the most successful phyla, lack clear evidence of adaptive immunity and yet thrive in the oceans, which are rich in viruses. There are thought to be nearly 120,000 species of Mollusca, most living in marine habitats. Despite the extraordinary abundance of viruses in oceans, molluscs often have very long life spans (10 to 100 years). Thus, their innate immunity must be highly effective at countering viral infections. Antiviral compounds are a crucial component of molluscan defenses against viruses and have diverse mechanisms of action against a wide variety of viruses, including many that are human pathogens. Antiviral compounds found in abalone, oyster, mussels, and other cultured molluscs are available in large supply, providing good opportunities for future research and development. However, most members of the phylum Mollusca have not been examined for the presence of antiviral compounds. The enormous diversity and adaptations of molluscs imply a potential source of novel antiviral compounds for future drug discovery.

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In vitro antiviral activity of antimicrobial peptides against herpes simplex virus 1, adenovirus, and rotavirus

Cited by 78 publications

References 25 publications

Clavanin A Improves Outcome of Complications from Different Bacterial Infections

Clavanin A Improves Outcome of Complications from Different Bacterial Infections

The fusion protein Penhrp containing Penaeidin-2 and HarpinZ induced a stronger hypersensitive response and systemic acquired resistance in tobacco comparing to the original HarpinZ

Marine Snails and Slugs: a Great Place To Look for Antiviral Drugs

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