The rapid increase in the incidence of multidrug-resistant infections today has led to enormous interest in antimicrobial peptides (AMPs) as suitable compounds for developing unusual antibiotics. In this study, clavanin A, an antimicrobial peptide previously isolated from the marine tunicate Styela clava, was selected as a purposeful molecule that could be used in controlling infection and further synthesized. Clavanin A was in vitro evaluated against Staphylococcus aureus and Escherichia coli as well as toward L929 mouse fibroblasts and skin primary cells (SPCs). Moreover, this peptide was challenged here in an in vivo wound and sepsis model, and the immune response was also analyzed. Despite displaying clear in vitro antimicrobial activity toward Gram-positive and -negative bacteria, clavanin A showed no cytotoxic activities against mammalian cells, and in acute toxicity tests, no adverse reaction was observed at any of the concentrations. Moreover, clavanin A significantly reduced the S. aureus CFU in an experimental wound model. This peptide also reduced the mortality of mice infected with E. coli and S. aureus by 80% compared with that of control animals (treated with phosphate-buffered saline [PBS]): these data suggest that clavanin A prevents the start of sepsis and thereby reduces mortality. These data suggest that clavanin A is an AMP that could improve the development of novel peptide-based strategies for the treatment of wound and sepsis infections.
The main bacterium associated with skin infection is Staphylococcus aureus, occurring especially in infections acquired via surgical wounds, commonly leading to lethal hospital-acquired infections, emphasizing the importance of identifying new antimicrobial compounds. Among them, cyclotides have gained interest due to their high stability and multifunctional properties. Here, cycloviolacin 2 (CyO2) and kalata B2 (KB2) were evaluated to determinate their anti-staphylococcal activities using a subcutaneous infection model. Anti-staphylococcal activities of 50mM for KB2 and 25mM for CyO2 were detected with no cytotoxic activities against RAW 264.7 monocytes. In the in vivo assays, both cyclotides reduced bacterial load and CyO2 demonstrated an increase in the phagocytosis index, suggesting that the CyO2 in vivo anti-staphylococcal activity may be associated with phagocytic activity, additionally to direct anti-pathogenic activity.
If in the 19th century scientific knowledge moved from a generalist perspective to a growing specialization, in recent decades, problems that transcend disciplinary and political boundaries have required solutions based on interdisciplinary research and global actions, which led to the establishment of the Sustainable Development Goals (SDGs). Viewing from the latter perspective, the study of ecosystem services has converged on a fast-growing, transdisciplinary area of knowledge, at the same time that the advances in the nuclear field have enabled applications in industry, health, agriculture and the environment. Considering the development of these two areas of knowledge, the objective of this study is to evaluate the correlation between Ecosystem Services (ES) and Nuclear Science and Technology (NST), by means of category building and content analysis applied to articles compiled from Web of Science. From 1980 to June 2020, 27,301 records (articles and reviews) were listed for the term “Ecosystem Service*”. When refining the result with the application of descriptors related to the nuclear field, correspondences were found for “Uranium”=14; “Nuclear Power”=6; “Nuclear Energy”=3; “Nuclear Technology*”=1; “Nuclear Fuel*”=1; “Nuclear Material*”=1; “Radiation”=7; “Isotope*”=188, totalizing 221 correspondences. On the other hand, 9,949 records were obtained for the same time interval, when using the descriptors for the nuclear field, plus the terms “Nature” or “Ecosystem*” or “Environment”. Despite attesting that NST truly converges on ES, this correlation needs to be made more explicit in ES studies, in order to expand the perspectives for the conservation, preservation and recovery of the ecosystem services and their contribution to human well-being.
Nuclear science and technology (NST) offers a multitude of applications and tools and has a high level of regulation. However, its contribution to the achievement of global development goals is still incipient. Although its application is directly related to many fields, especially sustainability, the current literature does not relate it to socio-environmental issues, hindering the adoption of public policies based on isotopic and nuclear solutions. In large and megacities, the promotion of human well-being and the conservation of ecosystems are urgent global challenges, especially as a function of the growing expansion of land use modification, water scarcity, and climate change. The relationship between society and NST is addressed in this study, which aims to show how and in what ways the emerging and innovative nuclear and isotopic solutions contribute to the urban dimension of the United Nations 2030 Agenda, expressed by SDG 11 (Sustainable Cities and Communities), and its connection to land, water, and climate change in cities. This gap in knowledge compromises the targeting of resources to improve NST as a development strategy. Demystifying NST and increasing collaboration between ecosystem services and other issues related to sustainability in cities are key to implementing global development policies, especially at the local governance level.
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