In vitro antitubercular activity of extract and constituents from the stem bark of Disthemonanthus benthamianus

Evina, Jean Noel; Bikobo, Dominique Serge Ngono; Zintchem, Auguste Abouem A; Nyemeck, Norbert Mbabi; Ndedi, Esther Del Florence Moni; Diboué, Patrick Hervé Betote; Nyegue, Maximilienne Ascension; Atchadé, Alex de Théodore; Pegnyemb, Dieudonné Emmanuel; Koert, Ulrich; Bochet, Christian G.

doi:10.1016/j.bjp.2017.09.006

Cited by 10 publications

(4 citation statements)

References 15 publications

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“…No antimycobacterial activity was reported earlier for the test compounds except for compound 4 which showed similar results against the resistant strain to isoniazid AC 45 (Evina et al. 2017 ; Tiam et al. 2017 ).…”

Section: Resultsmentioning

confidence: 66%

Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis

Nkot

Bikobo

Zintchem

et al. 2018

Pharmaceutical Biology

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Context: The roots of Lophira lanceolata Van Tiegh. Ex Keay (Ochnaceae) have numerous medicinal values in the Central African region. Even though the MeOH extract of the roots has shown antimycobacterial activities, the constituents responsible for this inhibitory activity remain unknown.Objective: Phytochemical investigation of the MeOH root extract of L. lanceolata and determination of the antimycobacterial activities of that extract and constituents against the growth of Mycobacterium tuberculosis.Materials and methods: Column chromatography was used to provide bioactive phytoconstituents. Those compounds were elucidated using MS and NMR spectroscopic data. Antimycobacterial screening of the extract (4.882–5000 µg/mL in DMSO during 24 h at 37 °C) and isolated compounds (0.244–250 µg/mL in DMSO during 24 h at 37 °C) was performed by microplate alamar blue assay (MABA) against two mycobacterial strains.Results: The investigation of L. lanceolata MeOH roots extract provided of mixture of unseparated biflavonoids with a newly described one, dihydrolophirone A (1a) associated to lophirone A (1b). The bioactive compounds that effectively inhibited the growth of M. tuberculosis AC45 were found to be compounds 1 and 2. They exhibited MIC values of 31.25 and 15.75 µg/mL, respectively, and their MIC was found to be 62.5 µg/mL against resistant strain AC83.Discussion and conclusions: It is clearly evident from the results obtained that the mycobacterial activity of L. lanceolata could be related mainly to its steroid and flavonoid contents. Therefore, this study suggests the potential of the above-mentioned classes of compounds as promising candidate agents for developing new anti-tuberculosis drugs.

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Section: Resultsmentioning

confidence: 66%

Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis

Nkot

Bikobo

Zintchem

et al. 2018

Pharmaceutical Biology

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“…Several other studies have shown terpenes to be responsible for antimycobacterial activity (Seidel & Taylor 2004;Aguiar et al 2005;Higuchi et al 2008;Subramaniam et al 2014;Evina et al 2017;Suja et al 2017). Their high lipophilicity is probably the main factor that allows their penetration through the mycobacterial cell wall (Higuchi et al 2008).…”

Section: Discussionmentioning

confidence: 94%

Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis

et al. 2020

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Duroia saccifera (Rubiaceae) occurs in the Amazon rainforest and their extracts showed antibacterial properties. To obtain greater quantities of active substances, leaf segments from in vitro D. saccifera seedlings were used as explants for calli induction; calli were multiplied via multiple subcultures, dried and extracted with hexane followed by ethyl acetate (EtOAc) and methanol (MeOH). As D. macrophylla had been reported to produce antimycobacterial substances, we assayed calli extracts against Mycobacterium tuberculosis (H37Rv strain). Calli EtOAc extract was active, with a minimal inhibitory concentration (MIC) of ≤ 25 mg mL-1, IC90of 19.5 mg mL-1 and minimal bactericidal concentration (MBC) of 200 mg mL-1. EtOAc extract was analyzed by Thin Layer Chromatography (TLC) and Nuclear Magnetic Resonance (NMR) to determine its chemical profile, and was found to be rich in terpenes. Chromatographic fractionation of the EtOAc extract yielded a mixture of two sterols, β-sitosterol and stigmasterol (in proportion of 2:1), which were identified by 1H and 13C NMR analysis. As far as we know, this is the first report of Duroia saccifera in vitro cell culture, antituberculosis activity of calli extract and β-sitosterol and stigmasterol isolation from in vitro plant cell culture.

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“…Gallic acid and its derivatives had been isolated from M. benthamianum (Tchinda et al, 2016). Gallic acid derivative isolated from another benthamianum species, Disthemonanthus benthamianum, had demonstrated antitubercular activity (Evina et al, 2017). Synthesized derivatives of gallic acid showed anti-tuberculosis activity (Ilango and Arunkumar, 2010).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of anti-Mycobacterium tuberculosis activity of fractions from selected medicinal plants used traditionally for treating cough and respiratory disorders in South West of Nigeria

Ademoye¹,

Lajide²,

Owolabi³

et al. 2018

J. Med. Plants Res.

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Tuberculosis is a contagious airborne infection that mostly affects the lungs. The causative agent of tuberculosis in human is Mycobacterium tuberculosis. The emergence and dissemination of M. tuberculosis isolates that are resistant to multiple antimicrobial drugs represent a growing public health threat. Fractions from Alafia barteri, Chasmanthera dependence, Chrysophyllum albidum, Emilia coccinea, Mezoneuron benthamianum, Phyllanthus muellerianus, Secamoni afzeli, Senna alata, Xylopia aethiopica and Acalypha fimbriata were screened for activity against drug susceptible M. tuberculosis H 37 Rv and the local isolates using proportion and nitrate reduction methods. The organisms used were M. tuberculosis H37Rv strain and the local isolates from TB patients. The standard antitubercular drugs used were isoniazid and rifampicin. No fractions from A. barterii, C. dependens, E. coccinea, S. afzeli, S. alata and X. aethiopica showed sensitivity against the M. tuberculosis strains. The hexane fraction of C. albidum, butanol fraction of M. benthamianum, ethyl acetate fraction of P. muellerianus and ethyl acetate fraction of A. fimbriata showed sensitivity with minimum inhibition concentration of 0.5 mg/ml. The ethylacetate and hexane fractions of M. benthamianum together with hexane fraction of P. muellerianus showed sensitivity with MIC value of 1.25 mg/ml. The highest MIC value of 2.5 mg/ml was obtained from hexane fraction of A. fimbriata. Thus, C. albidum, M. benthamianum, P. muellerianus and A. fimbriata possessed antimycobacterium tuberculosis activity and further research work would be required to assess possible antitubercular agents present in the four medicinal plants.

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In vitro antitubercular activity of extract and constituents from the stem bark of Disthemonanthus benthamianus

Cited by 10 publications

References 15 publications

Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis

Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis

Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis

Evaluation of anti-Mycobacterium tuberculosis activity of fractions from selected medicinal plants used traditionally for treating cough and respiratory disorders in South West of Nigeria

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