Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis

Abstract

Context: The roots of Lophira lanceolata Van Tiegh. Ex Keay (Ochnaceae) have numerous medicinal values in the Central African region. Even though the MeOH extract of the roots has shown antimycobacterial activities, the constituents responsible for this inhibitory activity remain unknown.Objective: Phytochemical investigation of the MeOH root extract of L. lanceolata and determination of the antimycobacterial activities of that extract and constituents against the growth of Mycobacterium tuberculosis.Materials and methods: Column chromatography was used to provide bioactive phytoconstituents. Those compounds were elucidated using MS and NMR spectroscopic data. Antimycobacterial screening of the extract (4.882–5000 µg/mL in DMSO during 24 h at 37 °C) and isolated compounds (0.244–250 µg/mL in DMSO during 24 h at 37 °C) was performed by microplate alamar blue assay (MABA) against two mycobacterial strains.Results: The investigation of L. lanceolata MeOH roots extract provided of mixture of unseparated biflavonoids with a newly described one, dihydrolophirone A (1a) associated to lophirone A (1b). The bioactive compounds that effectively inhibited the growth of M. tuberculosis AC45 were found to be compounds 1 and 2. They exhibited MIC values of 31.25 and 15.75 µg/mL, respectively, and their MIC was found to be 62.5 µg/mL against resistant strain AC83.Discussion and conclusions: It is clearly evident from the results obtained that the mycobacterial activity of L. lanceolata could be related mainly to its steroid and flavonoid contents. Therefore, this study suggests the potential of the above-mentioned classes of compounds as promising candidate agents for developing new anti-tuberculosis drugs.

Peer Review #1 of "Assessment of antimycobacterial activities of pure compounds extracted from Thai medicinal plants against clarithromycin-resistant Mycobacterium abscessus (v0.1)"

“…Researchers have isolated several such compounds and demonstrated their activities against mycobacteria, including M. tuberculosis (Baldin et al 2018;Chaipukdee et al 2016;Jimenez-Arellanes et al 2013;Jyoti et al 2016;Kanokmedhakul et al 2012;Molina-Salinas et al 2006;Naik et al 2014;Nkot et al 2018). However, antimycobacterial activities of secondary metabolites against M. abscessus have rarely been reported.…”

“…Plants are a source of bioactive compounds that can treat various diseases (Rios & Recio 2005). Several research teams have reported anti-Mycobacterium tuberculosis activity of extracts from Tetradenia riparia (Baldin et al 2018), Persea americana (Jimenez-Arellanes et al 2013), Lophira lanceolata (Nkot et al 2018) and Flourensia cernua (Molina-Salinas et al 2006).…”

Assessment of antimycobacterial activities of pure compounds extracted from Thai medicinal plants against clarithromycin-resistant Mycobacterium abscessus

“…Researchers have isolated several such compounds and demonstrated their activities against mycobacteria, including M. tuberculosis ( Baldin et al, 2018 ; Chaipukdee et al, 2016 ; Jimenez-Arellanes et al, 2013 ; Jyoti et al, 2016 ; Kanokmedhakul et al, 2012 ; Molina-Salinas et al, 2006 ; Naik et al, 2014 ; Nkot et al, 2018 ). However, antimycobacterial activities of secondary metabolites against M. abscessus have rarely been reported.…”

“…Plants are a source of bioactive compounds that can treat various diseases ( Rios & Recio, 2005 ). Several research teams have reported anti- Mycobacterium tuberculosis activity of extracts from Tetradenia riparia ( Baldin et al, 2018 ), Persea americana ( Jimenez-Arellanes et al, 2013 ), Lophira lanceolata ( Nkot et al, 2018 ) and Flourensia cernua ( Molina-Salinas et al, 2006 ). Northeastern Thailand has high plant diversity, which remains locally important as a source of traditional medicines ( Kaewpiboon et al, 2012 ).…”

Influence of Lophira lanceolata Leaf Extract on Reproductive Indices and Hematological Parameters in Male Wistar Rats

“…2 L. lanceolata is used for the treatment of toothache, liver infections, female sterility, cough, fever, heart pains, blood spitting, intercostals pain, stomach pain, dysmenorrhea, respiratory troubles, and to relieve the gripping of dysentery. 3 In Nigeria, it is used in the management of jaundice, measles and liver injury. 4,5 Traditional knowledge of indigenous communities about plant diversity makes them major contributors to primary health care for majority of the world population.…”