In vitro antioxidative and anti-inflammatory effects of the compound K-rich fraction BIOGF1K, prepared from Panax ginseng

Hossen, Muhammad Jahangir; Hong, Yong Deog; Baek, Ki Hyun; Yoo, Seunghyun; Hong, Yo Han; Kim, Ji Hye; Lee, Jeong-Oog; Kim, Dong-Hyun; Park, Junseong; Cho, Jae Youl

doi:10.1016/j.jgr.2015.12.009

Cited by 84 publications

(60 citation statements)

References 57 publications

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“…RAW264.7 cells were treated with escalating doses of Mc‐eWE (0–400 μg/ml) for 24 hr, and NO levels released in the cell culture media were measured using Griess reagent, as previously reported (Guevara et al, ; Hossen et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…2.7 | NO production assay RAW264.7 cells were treated with escalating doses of Mc-eWE (0-400 μg/ml) for 24 hr, and NO levels released in the cell culture media were measured using Griess reagent, as previously reported (Guevara et al, 1998;Hossen et al, 2017). 2.8 | Measuring mRNA expression levels by quantitative real-time PCR RAW264.7 cells and splenocytes were treated with escalating doses of Mc-eWE (0-400 μg/ml) for 24 and 12 hr, respectively. Total RNA was extracted using TRI reagent®, and cDNA was synthesized from 1 μg of total RNA using reverse transcriptase, according to the manufacturer's instructions.…”

Section: Cell Viability Assaymentioning

confidence: 99%

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Morinda citrifolia noni water extract enhances innate and adaptive immune responses in healthy mice, ex vivo, and in vitro

Hong

Han

et al. 2019

Phytotherapy Research

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Although Morinda citrifolia (noni) has long been used in traditional medicines for human diseases, its molecular and cellular mechanism of immunostimulatory ability to improve human health under normal healthy conditions is not fully elucidated. This study aimed to investigate the in vitro and in vivo immunostimulatory activity of M. citrifolia fruit water extract treated with enzymes (Mc‐eWE). In vitro studies revealed that Mc‐eWE stimulated the cells by inducing nitric oxide (NO) production and the expression of inflammatory cytokines, such as interleukin (IL)‐1β, IL‐6, IL‐12, tumor necrosis factor‐alpha (TNF‐α), and interferon‐gamma (IFN‐γ). The immunostimulatory activity was mediated by activation of NF‐κB and AP‐1. Ex vivo studies showed that Mc‐eWE stimulated splenocytes isolated from mice by inducing NO production and expression of immunostimulatory cytokines and by downregulating the expression of the immunosuppressive cytokine IL‐10 without cytotoxicity. In vivo demonstrated that Mc‐eWE induced immunostimulation by modulating populations of splenic immune cells, especially by increasing the population of IFN‐γ+ NK cells. Mc‐eWE enhanced the expression of inflammatory genes and immunostimulatory cytokines and inhibited the expression of IL‐10 in the mouse splenocytes and sera. Taken together, these results suggest that Mc‐eWE plays an immunostimulatory role by activating innate and adaptive immune responses.

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Section: Methodsmentioning

confidence: 99%

Section: Cell Viability Assaymentioning

confidence: 99%

Morinda citrifolia noni water extract enhances innate and adaptive immune responses in healthy mice, ex vivo, and in vitro

Hong

Han

et al. 2019

Phytotherapy Research

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“…The RAW264.7 cells or peritoneal macrophages were pre-treated with Rb-ME or L-NAME or Prednisolone (Pred) for 30 min., and then stimulated with LPS for 24 h. The supernatant (100 µL) obtained was mixed with 100 µL of Griess reagent, as reported previously [18,19]. The absorbance of this mixture was measured at 540 nm and the concentration of NO was calculated by comparison with a standard curve.…”

Section: Nitric Oxide (No) Assaymentioning

confidence: 99%

Ranunculus bulumei Methanol Extract Exerts Anti-Inflammatory Activity by Targeting Src/Syk in NF-κB Signaling

2020

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1) Background: Ranunculus bulumei is a flowering plant that belongs to the Ranunculus species. Several Ranunculus species, such as R. aquatilis and R. muricatus, have traditionally been used to treat fever and rheumatism throughout Asia, suggesting that plants belonging to the Ranunculus species may have anti-inflammatory effects. To our knowledge, the pharmacological activity of R. bulumei has not been reported. Therefore, in this study, we aim to assess the anti-inflammatory activity of a methanol extract that was derived from R. bulumei (Rb-ME) in macrophage-mediated inflammatory responses and to identify the molecular mechanism that underlies any anti-inflammatory action.(2) Methods: The anti-inflammatory efficacy of Rb-ME was evaluated while using in vitro and in vivo experiments. The RAW264.7 cells and peritoneal macrophages were stimulated by lipopolysaccharide (LPS). In addition, LPS-induced peritonitis and HCl/EtOH-triggered gastritis models were produced. A nitric oxide (NO) assay, real-time PCR, luciferase reporter gene assay, western blot analysis, plasmid overexpression strategy, and in vitro kinase assay were used to determine the molecular mechanisms and target molecules of Rb-ME. The phytochemical active ingredients of Rb-ME were also identified by high performance liquid chromatograph (HPLC). (3) Results: Rb-ME reduced the production of NO and mRNA expression of iNOS, COX-2, IL-1β, and IL-6 without cytotoxicity. The protein secretion of TNF-α and IL-6 was also decreased by Rb-ME. HPLC analysis indicates that quercetin, luteolin, and kaempferol are the main active ingredients in the anti-inflammatory efficacy of Rb-ME. Rb-ME also blocked MyD88-induced NF-κB promoter activity and nuclear translocation of NF-κB subunits (p65 and p50). Moreover, Rb-ME reduced the phosphorylation of IκBα, Akt, p85, Src, and Syk, which are NF-κB upstream signaling molecules in LPS-activated RAW264.7 cells. According to the in vitro kinase assay, Rb-ME directly inhibits Syk kinase activity. The oral administration of Rb-ME alleviated inflammatory responses and the levels of p-IκBα in mice with LPS-induced peritonitis and HCl/EtOH-induced gastritis. (4) Conclusions Rb-ME has anti-inflammatory capacity by suppressing NF-κB signaling and it has been found to target Src and Syk in the NF-κB pathway. Based on this efficacy, Rb-ME could be developed as an anti-inflammatory herbal medicine.

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“…In another study, the in vitro antioxidative and anti‐inflammatory effects of BIOGF1K, a compound K‐rich fraction isolated from the root of Panax g inseng, were accessed, being observed a significant suppression of IKKβ and TBK1, involved respectively, in the transcriptional regulation of NF‐κB and IRF3 . Moreover, the microbial transformation of ginsenoside Rb1, Re and Rg1 increased their anti‐inflammatory properties via lipopolysaccharide‐stimulated murine RAW 264.7 macrophages and even xylene‐induced acute inflammatory model of mouse ear oedema .…”

Section: Major Medicinal Plants Used In Medication System As Anti‐agementioning

confidence: 99%

Brief Challenges on Medicinal Plants: An Eye‐Opening Look at Ageing‐Related Disorders

Bjørklund

Dadar

Martins

et al. 2018

Basic Clin Pharma Tox

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Several studies have reported that nature-derived antioxidants may prevent free radicals over-production and therefore control the onset and prevent the exacerbation of different kinds of diseases caused by oxidative stress and redox-derived stressors, including ageing, fundamentally by suppressing the oxidative by-products-mediated degradation. Naturally derived antioxidants exert their anti-ageing action via a panoply of signalling systems, many of which engaging reactive oxygen and nitrogen species scavenging, with the Nrf2/Keap1-ARE system and improving the many survival genes and functions (such as the pathway mTOR/Foxo/SIRT1) able to slow cellular senescence. Most of the research in this field has evaluated the regulative effects and even pathways of herbal extracts with antioxidant property in the ageing process, and various age-related disorders such as cardiovascular disease, ischaemia-reperfusion injury, coronary and myocardial circulatory perfusion, peripheral vascular resistance, and even neurodegenerative disorders are prevented plant phytochemicals often via their antioxidant potential. A much more complex ability to interact with survival functions makes these compounds successfully active in preventing ageing-related disorders. This report aimed to discuss in more detail some selected medicinal plants including Allium sativum, Aloe vera, Crataegus spp., Cynara scolymus, Eleutherococcus senticosus, Ginkgo biloba, Hippophae rhamnoides, Panax ginseng, Rosmarinus officinalis, Schizandra chinensis, Vitis vinifera and seaweeds in the prevention of ageing-related pathologies. A systematic overview of the relevant information in the antioxidant function of the many herbal products reviewed here for the control of the ageing process is proposed, to provide a new horizon on the design of anti-ageing herbal medicines.

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In vitro antioxidative and anti-inflammatory effects of the compound K-rich fraction BIOGF1K, prepared from Panax ginseng

Cited by 84 publications

References 57 publications

Morinda citrifolia noni water extract enhances innate and adaptive immune responses in healthy mice, ex vivo, and in vitro

Morinda citrifolia noni water extract enhances innate and adaptive immune responses in healthy mice, ex vivo, and in vitro

Ranunculus bulumei Methanol Extract Exerts Anti-Inflammatory Activity by Targeting Src/Syk in NF-κB Signaling

Brief Challenges on Medicinal Plants: An Eye‐Opening Look at Ageing‐Related Disorders

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