In vitro Antioxidant, Antihyperglycaemic and Antihyperlipidaemic Activities of Ethanol Extract of Lawsonia inermis Leaves

Ojewunmi, Oyesola

doi:10.9734/bjpr/2014/6359

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…Moreover, other recent studies, using as Ojewunmi et al [34], using phenolic compounds extracts from Lawsonia inermis have reported a concentration of inhibition at 50% (of DPPH) with 49.22 μg/mL via an ethanolic extract of leaves. Similar work carried by Guha et al [35] and Enneb et al [36] reported concentrations of 32.87 μg/mL and 25.73 μg/mL, respectively, of methanolic extracts of this same species.…”

Section: Discussionmentioning

confidence: 99%

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Elaguel¹,

Kallel²,

Gargouri

et al. 2019

Lipids Health Dis

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BackgroundThe present study was focused on the optimization of yield of the essential oil extraction from leaves of Lawsonia inermis, and the determination of chemical composition, antioxidant activities, and lipid peroxydation and antiproliferative effects.MethodsHenna essential oil (HeEO) were extracted by hydrodistillation; the identification of the chemical composition were done by GC/MS method. HeEO was analyzed for antioxidant power in: (1) chemical system by the DPPH test, the ABTS test and the total antioxidant activity test; and (2) in biological system by lipid peroxydation tests (MDA and DC) in cells culture. The cytotoxicity effects of HeEO were assessed using MTT assay against Raji and HeLa cell lines.ResultsThe optimal extraction yield was 6.8 g/100 g d.b. HeEO showed a remarkable anti-oxidant activities including DDPH (42%), ABTS (87%) and the power of ammonium phosphomolybdate (2992 ± 230 mg of HeEO by equivalent to 1 mg of vitamin C in terms of total antioxidant power).ConclusionBeyond notable antioxidant activities of the HeEo, our results showed a significant decrease in the production of ERO in the Raji cell line. The anti-tumor power of the Henna essential oil shows an interesting cytotoxicity effect (IC50 at 0.26 μg/mL for Raji and at 1.43 μg/mL for HeLa) with a total mortality percentage reaching 60%, for both.

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Section: Discussionmentioning

confidence: 99%

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Elaguel¹,

Kallel²,

Gargouri

et al. 2019

Lipids Health Dis

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show abstract

“…It is also possible that any of these phytochemicals could be associated with the antihyperlidipemic effect of the plant. The antihyperlipidaemic effect might have resulted from decreased fatty acid concentration in the circulation and reduced cholesterol synthesis possibly due to the flavonoid constituents of the plant extract (Ojewunmi et al, 2014). The actual mechanism of action and the phytoconstituent responsible for the observed effects of the plant, however, needs to be investigated further.…”

Section: Resultsmentioning

confidence: 99%

Antidiabetic activity and phytochemical screening of Acalypha wilkesiana (Euphorbiaceae) Mull Arg. roots in alloxan-induced diabetic rats

Odoh¹,

Ndubuokwu²,

.³

et al. 2014

Sci. Res. Essays

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The aim of this study was to investigate the anti-diabetic effects and biochemical parameters of methanol root extract of Acalypha wilkesiana Mull Arg. (MEAW) in alloxan-induced diabetic rats. The effect of the extract (200 and 400 mg/kg, p.o.) on fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) level and liver glycogen content were investigated in alloxan-induced diabetic rats after 14 days. An oral glucose tolerance test (OGTT) was also performed on the diabetic rats. Dose selection was made on the basis of acute oral toxicity study. Phytochemical analysis of the root extract was carried out following standard procedures. The most significant (p<0.05) reduction of FBG level of 74.06% was observed for 400 mg/kg in alloxan induced diabetic rats. A significant reduction (p<0.05) in serum TC and TG level of 50.43 and 58.05% respectively was also observed for the high dose of the extract. The SGOT and SGPT levels were significantly (p<0.05) reduced. The MEAW also showed improvement of body weight in diabetic rats. The animals showed no mortality at a dose of 5000 mg/kg while results of phytochemical analysis revealed the presence of mainly alkaloids, terpenoids, flavonoids, saponins, steroids and tannins. These results show that the root of Acalypha wilkesiana possesses antidiabetic, antihyperlipidemic and hepatoprotective effects.

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“…The total flavonoid content was estimated by using the slightly modified colorimetric method originally described by Woisky and Salatin 49 and applicable for glycosylated or non-glycosylated flavonoids determination 50 . An aliquot (1.5 mL) of the extract was mixed with 3 mL of methanol, 0.2 mL of 10% aluminum chloride, 0.2 mL of 1 M potassium acetate, and 5.6 mL of distilled water.…”

Section: Methodsmentioning

confidence: 99%

Multivariate analysis of polyphenolic content and in vitro antioxidant capacity of wild and cultivated berries from Bosnia and Herzegovina

Marjanović

Djedjibegović

Lugusić

et al. 2021

Sci Rep

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The aim of this study was to determine the antioxidant activity, total phenolics, total flavonoid, proanthocyanidins, and anthocyanins content of eight berry species, namely serviceberry, gooseberry, blackberry, black chokeberry, bilberry, red currant, black currant, and cornelian cherry harvested in the regions of Sarajevo and Tuzla, Bosnia and Herzegovina. The antioxidant activity was determined by a battery of in vitro tests including DPPH radical assay, FRAP assay, ABTS assay, and phosphomolybdate test for total antioxidant capacity. Total phenolics, total flavonoids, and proanthocyanidins ranged from 0.834 to 6.921 mg TAE (tannic acid equivalents), 0.081–0.673 mg of quercetin, and 0.162–3.247 mg of catechin per gram of fresh fruit, respectively. The water extract of fruits had considerable levels of tested constituents and antioxidant activity, with the highest results obtained for black chokeberry. The multivariate clustering analysis showed that water extracts of analyzed species of berries belong to four distinct types in terms of their antioxidants levels and antioxidant activity. Furthermore, these results support the opinion that employment of multiple antioxidant tests is indeed required for adequate in vitro assessment of antioxidant capacity. Results also emphasized the need for a more detailed evaluation of the fruit species with good antioxidant potential (relative to standards), such as cornelian cherry and gooseberry, which are abundant yet not frequently consumed in Bosnia and Herzegovina.

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In vitro Antioxidant, Antihyperglycaemic and Antihyperlipidaemic Activities of Ethanol Extract of Lawsonia inermis Leaves

Cited by 11 publications

References 27 publications

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Antidiabetic activity and phytochemical screening of Acalypha wilkesiana (Euphorbiaceae) Mull Arg. roots in alloxan-induced diabetic rats

Multivariate analysis of polyphenolic content and in vitro antioxidant capacity of wild and cultivated berries from Bosnia and Herzegovina

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