In-Vitro Antioxidant and in-Vivo Hepato Protective Activity of Ethanol Extracts From the Bark of Shorea Robusta (Dipterocarpaceae) Against Carbon Tetra Chloride Induced Liver Toxicity in Rats

Biswas, Diptanu

doi:10.22159/ajpcr.2016.v9i6.14271

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…This elevation was attributed to the leakage of enzymes from cells into the circulatory system due to altered permeability and loss of functional integrity of liver membrane as a result of severe hepatic injury [42,43]. The results obtained in this work are similar to findings of Biswas et al [44]. CCl 4 undergoes reductive metabolism by the hepatic cytochrome P450 into a highly reactive + CCl 3 , which is converted into peroxyl radical ( + OOCCl 3 ) in the presence of oxygen [45].…”

Section: Discussionsupporting

confidence: 86%

Attenuation of Carbon Tetrachloride and Ethanol-Induced Hepatic Fibrosis in Rats by Calligonum Comosum Shoot Extract

Mousa¹,

Hassan²,

El-Sammad³

2017

Asian J Pharm Clin Res

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Objective: This study was designed to evaluate the hepatoprotective and antioxidant effects of methanolic extract of Calligonum comosum (C. comosum) shoots on the hepatic fibrosis induced by carbon tetrachloride and ethanol (CCl 4 /ethanol) in rats.Methods: A liver fibrosis was induced in Sprague Dawley rats by oral administration of CCl 4 (1 ml/kg body weight, twice weekly for 10 weeks) along with ethanol (10% in drinking water 1 week before CCl 4 administration and throughout the experiment). Rats were pretreated with C. comosum extract (daily, orally at a dose of 50 mg/kg body weight 1 week before CCl 4 administration). At the end of the experiment, serum, and liver samples were subjected to biochemical investigations. In addition, liver and kidney tissues were evaluated for histopathological changes.Results: C. comosum extract pretreatment significantly reduced CCl 4 -induced elevation in serum levels of aspartate aminotransaminase, alanine aminotransaminase, alkaline phosphatase, total bilirubin, urea, creatinine, and significantly elevated serum contents of total protein and albumin, as well as an improvement in hepatic protein content, albumin/globulin ratio, body weight and relative liver and kidney weights. C. comosum extract also significantly increased the hepatic levels of glucose-6-phosphatase, glutathione peroxidase, glutathione-S-transferase, catalase, superoxide dismutase, and glutathione with significant decrease in the contents of malondialdehyde and protein carbonyl. In addition, downregulation in expression of the fibrotic marker matrix metalloproteinase-2 was observed. C. comosum extract also ameliorated histopathological changes of CCl 4 /ethanol group which further evidenced the biochemical findings. Conclusion:Our results confirmed that the methanolic extract of C. comosum shoots effectively protect against CCl 4 /ethanol-induced liver fibrosis, through its antioxidant property.

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Section: Discussionsupporting

confidence: 86%

Attenuation of Carbon Tetrachloride and Ethanol-Induced Hepatic Fibrosis in Rats by Calligonum Comosum Shoot Extract

Mousa¹,

Hassan²,

El-Sammad³

2017

Asian J Pharm Clin Res

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“…Briefly, 3 mL of extract with different concentration (50 mg/mL, 100 mg/mL, 150 mg/mL, 250 mg/mL, and 500 mg/mL) was mixed with 1 mL of DPPH (0.1 mM) solution in ethanol. The mixture was shaken vigorously and left to stand for 30 min in the dark at room temperature, and the absorbance was then measured with a quartz glass cuvette (Hellma; Mullheim, Germany) at 517 nm against a blank using an ultraviolet (UV)-visible spectrophotometer (Pharma Spec UV-1700; Shimadzu; Kyoto, Japan) [25]. A low absorbance of the reaction mixture indicated a high free radical scavenging activity.…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Evaluation of “Leucas Lanata” and Assessment of Its Hepatoprotective Effects on Antitubercular Drug-Induced Hepatic Damage

Verma

Srivastava

Rao

2018

Asian J Pharm Clin Res

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Objective: The objective of this study was to investigate the hepatoprotective activity of ethanolic whole plant extract of Leucas lanata (family - Lamiaceae) against rifampicin+isoniazid (RIF+INH) induced hepatic damage in rats.Methods: Wistar rats of either sex were divided into five groups of six animals each and given orally the following treatment for 28 days. The normal control was given 1% CMC 1 ml/kg body weight (b.w.), RIF+INH antitubercular drug (ATT) at a dose of 50 mg/kg b.w., p.o. was given a toxic dose for inducing hepatotoxicity. Silymarin (100 mg/kg, p.o) was given as reference standard. Two different doses of Lucas lanata extract of 200 and 400 mg/kg, p.o were tested for hepatoprotective activity. At the end of the treatment, blood was collected from direct cardiac puncture and analyzed for numerous bodily fluid parameter such as alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphate (ALP), total protein (TP), and total bilirubin (TB) and oxidative stress parameter lipid peroxidation (LPO), superoxide dismutase (SOD), catalase, glutathione (GSH), malondialdehyde and also evaluated the hydroxyproline content, tumor necrosis factor alpha, and interleukin-1 beta in all groups. Livers were isolated for its essential estimation followed by histopathological studies.Results: Hepatic serum markers (ALT, AST, ALP, albumins, TP, and TB) were significant (p<0.01–p<0.001) protective effect was obtained against ATT-induced liver damage, and ATT treatment significantly decreased LPO, SOD, and GSH (p<0.001) levels compared with control Group I and given a Leucas lanata extract (LLE) (p<0.01–p<0.001), the oxidative stress markers were markedly reversed. Histopathology of liver tissue showed that LLE attenuated the hepatocellular necrosis, regeneration, and repair of cells toward normal. LLE which was standardized using high-performance thin-layer chromatography revealed the presence of some critical phenolic (gallic acid and quercetin) compound.Conclusion: The results of this study powerfully indicate the protecting result of LLE against liver injury which can be attributed to its hepatoprotective activity, and thereby scientifically support its traditional use.

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Depression symptoms and associated factors among thalassemia patients in the Palestinian Territories: a cross-sectional study

Sarhan

Modallal

Mahamid

et al. 2022

Middle East Curr Psychiatry

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Background Thalassemia is a condition that causes the human body to destroy red blood cells faster than they can be made. It causes physical symptomology as well as psychological distress. The current study aimed to identify the prevalence of depression symptoms among individuals with moderate to severe thalassemia. A quantitative descriptive cross-sectional design was applied utilizing the Beck Depression Inventory to assess the level of depression in the sample. Results Some significant relationships were demonstrated in the sample: males reporting severe depression symptoms than females by 6.0 times (95% CI 0.07–0.62, p 0.014), and reporting moderate depression symptoms (95% CI 0.00–0.47, p 0.05). Additionally, income was found to be a predictor of level of depression symptoms with low income reported higher incidence of severe depression symptoms than those whose monthly income was > 400$ by 18.4 times (95% CI 0.38–1.03, p ≤ 0.001), but not reporting a significant association between moderate depressive symptoms and monthly income (95% CI − 012–1.47, P 0.225). A significant association was found between the educational attainment and level of depression symptoms with lower levels of education predicting higher levels of severe depression symptoms (95% CI 0.069–0.89, p 0.022), but no significant association was reported between moderate depression symptoms and educational attainment (95% CI − 0.49–0.01, p 0.81). Related to disease characteristics a relationship was confirmed between severe depressive symptoms and medication level such as the individual needing tablets or pumps (95% CI 0.189–1.05, p 0.005 and 95% CI 0.52–1.44, p 0.001) respectively. Other disease related variables showed no significant correlation with depressive symptom levels. Additional significant relationships were found in environment whereas increased moderate depressive symptoms were experienced by individuals residing in rural areas as opposed to those living in IDP camps or cities by 4 times (95% CI − 0.30–0.01, p 0.04), but no significant association was found between severe depression and the place of residence. The other independent variables had no significant correlation with severe or moderate depressive symptoms. Conclusion In light of these findings addressing symptoms of depression directly and supporting patients with thalassemia with basic life needs unrelated to their disease maybe mitigating depressive symptoms which may negatively affect recovery.

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In-Vitro Antioxidant and in-Vivo Hepato Protective Activity of Ethanol Extracts From the Bark of Shorea Robusta (Dipterocarpaceae) Against Carbon Tetra Chloride Induced Liver Toxicity in Rats

Cited by 3 publications

References 13 publications

Attenuation of Carbon Tetrachloride and Ethanol-Induced Hepatic Fibrosis in Rats by Calligonum Comosum Shoot Extract

Attenuation of Carbon Tetrachloride and Ethanol-Induced Hepatic Fibrosis in Rats by Calligonum Comosum Shoot Extract

Evaluation of “Leucas Lanata” and Assessment of Its Hepatoprotective Effects on Antitubercular Drug-Induced Hepatic Damage

Depression symptoms and associated factors among thalassemia patients in the Palestinian Territories: a cross-sectional study

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