In vitro Antioxidant and Cytotoxicity Analysis of Leaves of Ficus racemosa

Abstract

Objectives: The present study assessed the phytochemical components, In vitro antioxidant ability and cytotoxicity of leaf extract of Ficus racemosa. Methods: Preliminary phytochemical analysis in aqueous and ethanol was carried out for the presence of phytochemical components. Of the two extracts used ethanolic extract possessed the highest phytochemical constituents compared to aqueous extract. Hence the antioxidant activity of ethanolic extract of F. racemosa was performed by several antioxidant assays including 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay, nitric oxide (NO) scavenging assay, reducing power and superoxide radical (SO) scavenging assay. Results: From the results, F. racemosa has been found to have the significant antioxidant activity in a dose-dependent manner and IC 50 value was 150 μg/ml for DPPH and 100 μg/ml for both NO and SO scavenging assays. Further, the cytotoxicity analysis was determined against Dalton Lymphoma Ascites (DLA) cell line and the IC 50 value was found to be 175 μg/ml for ethanolic leaf extract of F. racemosa. Conclusion: Hence, the current study attests that F. racemosa is enriched in phytochemicals and a fine source of natural antioxidants with anticancer agents and can be used in pharmaceutical preparations for the treatment of diseases induced by oxidative stress.

Determination of in vivo biological activities of Dodonaea viscosa flowers against CCL4 toxicity in albino mice with bioactive compound detection

“…The quenching of DPPH measurement relies on the discoloration of the purple-colored 2,2-diphenyl-2-picryl-hydroxyl compound by antioxidant. Donor antioxidant decolorized DPPH radical by electron acceptance and measured quantitatively from variations in absorbance 74 . Furthermore, D. viscosa expressed significant radical scavenging activity against ABTS assay with a low value of IC 50.…”

Protective effect ofMonodora myristicaextracts on crude petroleum oil-contaminated catfish (Clarias gariepinus) diet in rats

“…Exposure of human and animals to these chemicals depends on different stage of CPO usage and environmental level [4] . Crude petroleum oil has been reported as a mediator in oxidative stress [5] , which may lead to various dreadful diseases like cancer [6] , dementia, atherosclerosis, multiple sclerosis, cardiac dysfunction, blood disorders, hepatic morphological abnormalities [6] , nephrotoxic effects [7] , etc.…”

Determination of in Vivo Biological Activities of Dodonaea Viscosa Flowers Against CCL4 Toxicity in Albino Mice With Bioactive Compound Detection

“…DPPH comprises odd electron gives absorbance at 515nm. Donor antioxidant decolorizes DPPH radical by electron acceptance, can be measured quantitatively from variations in absorbance [83]. Furthermore, D. Viscosa expressed significant radical scavenging activity against ABTS assay with low value of IC50.…”