In vitro antimicrobial photodynamic inactivation of multidrug-resistant Acinetobacter baumannii biofilm using Protoporphyrin IX and Methylene blue

Anane, Yaw Adjei; Apalata, Teke; Vasaikar, Sandeep; Okuthe, Grace Emily; Songca, Sandile P.

doi:10.1016/j.pdpdt.2020.101752

Cited by 14 publications

(15 citation statements)

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“…Multidrug-resistant was detected in more than 80% of all the A. baumannii isolates. The high MDR rates are in accordance with other South African studies (Lowings et al, 2015;Lowe et al, 2018;Law et al, 2019;Von Knorring et al, 2019; 10.3389/fmicb.2022.863129 Anane et al, 2020;Nogbou et al, 2022). The detection of nine colistin resistance isolates (9.4%) is worrisome as this is one of the last-resort antibiotics.…”

Section: Resistancenodulation-division Adeabc and Adefghsupporting

confidence: 91%

“…Acinetobacter baumannii was the most prevalent in infant patients (<1 year). Anane et al (2020) also reported that A. baumannii infections were the most prevalent in paediatric patients (0-9 years) in the Eastern Cape, South Africa. However, another South African study conducted in a tertiarylevel hospital in Gauteng from 2016 to 2017 showed that A. baumannii was most frequently isolated in adult patients (85.2%), followed by paediatric patients (62.5%) (Majavie et al, 2021).…”

Section: Resistancenodulation-division Adeabc and Adefghmentioning

confidence: 91%

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Molecular characterisation of Acinetobacter baumannii isolates from bloodstream infections in a tertiary-level hospital in South Africa

et al. 2022

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Acinetobacter baumannii is an opportunistic pathogen and causes various infections in patients. This study aimed to describe the clinical, epidemiological and molecular characteristics of A. baumannii isolated from BCs in patients at a tertiary-level hospital in South Africa. Ninety-six isolates from bloodstream infections were collected. Clinical characteristics of patients were recorded from patient files. Organism identification and AST was performed using automated systems. PCR screening for the mcr-1 to mcr-5 genes was done. To infer genetic relatedness, a dendrogram was constructed using MALDI-TOF MS. All colistin-resistant isolates (n = 9) were selected for WGS. The patients were divided into three groups, infants (<1 year; n = 54), paediatrics (1–18 years; n = 6) and adults (≥19 years; n = 36) with a median age of 13 days, 1 and 41 years respectively. Of the 96 A. baumannii bacteraemia cases, 96.9% (93/96) were healthcare-associated. The crude mortality rate at 30 days was 52.2% (48/92). The majority of the isolates were multidrug-resistant (MDR). All isolates were PCR-negative for the mcr-1 to mcr-5 genes. The majority of the isolates belonged to cluster 1 (62/96) according to the MALDI-TOF MS dendrogram. Colistin resistance was confirmed in nine A. baumannii isolates (9.4%). The colistin-resistant isolates belonged to sequence type (ST) 1 (5/6) and ST2 (1/6). The majority of ST1 isolates showed low SNP diversity (≤4 SNPs). All the colistin-resistant isolates were resistant to carbapenems, exhibited an XDR phenotype and harboured the blaOXA–23 gene. The blaNDM gene was only detected in ST1 colistin-resistant isolates (n = 5). The lpsB gene was detected in all colistin-resistant isolates as well as various efflux pump genes belonging to the RND, the MFS and the SMR families. The lipooligosaccharide OCL1 was detected in all colistin-resistant ST1 and ST2 isolates and the capsular polysaccharide KL3 and KL17 were detected in ST2 and ST1 respectively. This study demonstrated a 9.4% prevalence of colistin-resistant ST1 and ST2 A. baumannii in BC isolates. The detection of the lpsB gene indicates a potential threat and requires close prospective monitoring.

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Section: Resistancenodulation-division Adeabc and Adefghsupporting

confidence: 91%

Section: Resistancenodulation-division Adeabc and Adefghmentioning

confidence: 91%

Molecular characterisation of Acinetobacter baumannii isolates from bloodstream infections in a tertiary-level hospital in South Africa

et al. 2022

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“…Besides antimicrobials, natural compounds such as chitosan have been studied in combination with PDT to effectively eradicate biofilm-associated A. baumannii cells ( Zhang et al., 2019 ; Fekrirad et al., 2021 ). Another study showed the efficacy of methylene blue and protoporphyrin IX as the antibacterial photodynamic therapy photosensitizer against A. baumannii biofilms and methylene blue showed relatively significant reduction in colony forming units ( Anane et al., 2020 ). Recently Ran et al.…”

Section: Emerging Therapeutic Strategiesmentioning

confidence: 99%

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Upmanyu

Haq

Singh

2022

Current Research in Microbial Sciences

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“…Additionally, aPDT completely eradicated Gram-negative Moraxella catarrhalis and Gram-positive Streptococcus pneumoniae in both their planktonic and biofilm forms using the photosensitizer Chlorin-e6 and 670 nm light for excitation of the photosensitizer [ 63 ]. Biofilm-forming Acinetobacter baumannii planktonic cells and biofilms were eradicated using methylene blue and protoporphyrin IX photosensitizers, illuminated at 652 nm for excitation of the photosensitizer [ 64 ]. These studies show that aPDT is effective in eradicating biofilm-forming bacterial strains in both their planktonic and biofilm forms.…”

Section: Antimicrobial Photodynamic Therapymentioning

confidence: 99%

Applications of Antimicrobial Photodynamic Therapy against Bacterial Biofilms

Songca

Adjei

2022

IJMS

Self Cite

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Antimicrobial photodynamic therapy and allied photodynamic antimicrobial chemotherapy have shown remarkable activity against bacterial pathogens in both planktonic and biofilm forms. There has been little or no resistance development against antimicrobial photodynamic therapy. Furthermore, recent developments in therapies that involve antimicrobial photodynamic therapy in combination with photothermal hyperthermia therapy, magnetic hyperthermia therapy, antibiotic chemotherapy and cold atmospheric pressure plasma therapy have shown additive and synergistic enhancement of its efficacy. This paper reviews applications of antimicrobial photodynamic therapy and non-invasive combination therapies often used with it, including sonodynamic therapy and nanozyme enhanced photodynamic therapy. The antimicrobial and antibiofilm mechanisms are discussed. This review proposes that these technologies have a great potential to overcome the bacterial resistance associated with bacterial biofilm formation.

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In vitro antimicrobial photodynamic inactivation of multidrug-resistant Acinetobacter baumannii biofilm using Protoporphyrin IX and Methylene blue

Cited by 14 publications

References 58 publications

Molecular characterisation of Acinetobacter baumannii isolates from bloodstream infections in a tertiary-level hospital in South Africa

Molecular characterisation of Acinetobacter baumannii isolates from bloodstream infections in a tertiary-level hospital in South Africa

Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics

Applications of Antimicrobial Photodynamic Therapy against Bacterial Biofilms

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